Restriction map for pF1KB3488
##################################
# NO CUT ENZYMES (  146 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AcyI        
AfaI        AflII       AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BciVI       BfrI        BglI        BglII       BlpI        
BmyI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsiEI       BsiHKAI     BsiWI       BsmBI       BsoBI       
Bsp1286I    Bsp1407I    BspEI       BspHI       BspLU11I    
BsrBI       BsrGI       BssHII      BssSI       Bst1107I    
BstEII      BstPI       BstZ17I     CelII       CpoI        
DraI        DraII       DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        FspI        HaeII       HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PvuI        RcaI        RsaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AclI          610  AACGTT               AA^CGTT
AcsI          342  GAATTT               [GA]^AATT[CT]
AlwNI         920  CAGTTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          185  CCTCAGG              CC^T[ACGT]AGG
ApoI          342  GAATTT               [GA]^AATT[CT]
AseI          478  ATTAAT               AT^TAAT
AsnI          478  ATTAAT               AT^TAAT
AvrII         573  CCTAGG               C^CTAGG
BbvCI         993  GCTGAGG              CCTCAGC(-5/-2)
BcgI          228  CGAGAATCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          574  CTAG                 C^TAG
BlnI          573  CCTAGG               C^CTAGG
BsgI          637  CTGCAC               GTGCAG(16/14)
BsmAI         298  GAGAC                GTCTC(1/5)
BsmFI         662  GTCCC                GGGAC(10/14)
BspDI         695  ATCGAT               AT^CGAT
BspMI         760  GCAGGT               ACCTGC(4/8)
BsrFI         519  ACCGGC               [GA]^CCGG[CT]
BstAPI        150  GCAGGAAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI          98  TTCGAA               TT^CGAA
BstF5I        456  GGATG                GGATG(2/0)
BstXI         941  CCATGACAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         428  GGATCT               [GA]^GATC[CT]
Bsu36I        185  CCTCAGG              CC^T[ACGT]AGG
Cac8I          48  GCCCGC               GC[ACGT]^[ACGT]GC
Cfr10I        519  ACCGGC               [GA]^CCGG[CT]
ClaI          695  ATCGAT               AT^CGAT
DsaI          529  CCATGG               C^C[GA][CT]GG
EaeI          199  CGGCCA               [CT]^GGCC[GA]
Eco81I        185  CCTCAGG              CC^T[ACGT]AGG
FauI           49  CCCGC                CCCGC(4/6)
FokI          456  GGATG                GGATG(9/13)
HphI          670  GGTGA                GGTGA(8/7)
MaeI          574  CTAG                 C^TAG
MflI          428  GGATCT               [GA]^GATC[CT]
NcoI          529  CCATGG               C^CATGG
NlaIV         937  GGCCCC               GG[ACGT]^[ACGT]CC
NspV           98  TTCGAA               TT^CGAA
PflMI         919  CCAGTTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          890  GAGTC                GAGTC(4/5)
PshBI         478  ATTAAT               AT^TAAT
Psp1406I      610  AACGTT               AA^CGTT
SapI         1003  GAAGAGC              GCTCTTC(1/4)
SfaNI         673  GATGC                GCATC(5/9)
SfuI           98  TTCGAA               TT^CGAA
Tsp45I        242  GTCAC                ^GT[GC]AC
Van91I        919  CCAGTTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         428  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccII          51  CGCG                 CG^CG
               90  CGCG                
BclI          776  TGATCA               T^GATCA
              791  TGATCA              
BcnI           35  CCCGG                CC^[GC]GG
              664  CCCGG               
BsmI          617  GAATGC               GAATGC(1/-1)
              563  GCATTC              
BstUI          51  CGCG                 CG^CG
               90  CGCG                
CfoI           52  GCGC                 GCG^C
              652  GCGC                
Cfr13I        135  GGCCC                G^G[ACGT]CC
              937  GGCCC               
EcoT14I       529  CCATGG               C^C[AT][AT]GG
              573  CCTAGG              
FbaI          776  TGATCA               T^GATCA
              791  TGATCA              
HhaI           52  GCGC                 GCG^C
              652  GCGC                
HinP1I         52  GCGC                 G^CGC
              652  GCGC                
MaeII         611  ACGT                 A^CGT
              660  ACGT                
MseI          346  TTAA                 T^TAA
              479  TTAA                
MspA1I         57  CAGCTG               C[AC]G^C[GT]G
              272  CAGCTG              
MvnI           51  CGCG                 CG^CG
               90  CGCG                
NciI           35  CCCGG                CC^[GC]GG
              664  CCCGG               
PvuII          57  CAGCTG               CAG^CTG
              272  CAGCTG              
Sau96I        135  GGCCC                G^G[ACGT]CC
              937  GGCCC               
StyI          529  CCATGG               C^C[AT][AT]GG
              573  CCTAGG              
TaqI           99  TCGA                 T^CGA
              696  TCGA                
TfiI          231  GAATC                G^A[AT]TC
              287  GAATC               
Tru9I         346  TTAA                 T^TAA
              479  TTAA                
TthHB8I        99  TCGA                 T^CGA
              696  TCGA                
XcmI          529  CCATGGGGTGTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              854  CCAAACCCAAGTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AciI        AluI        AlwI        BbvI        BpmI        
BsaJI       BsiYI       BslI        BsrDI       BsrI        
BssKI       BstNI       DdeI        DpnII       EarI        
EcoRII      Fnu4HI      HaeIII      HapII       HinfI       
HpaII       ItaI        Ksp632I     MaeIII      MboI        
MboII       MnlI        MslI        MspI        MvaI        
MwoI        NdeII       NlaIII      PstI        Sau3AI      
ScrFI       SfcI        TseI        Tsp509I     TspRI       
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