Restriction map for pF1KB3450
##################################
# NO CUT ENZYMES (  133 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaII       
AvrII       BamHI       BanI        BbeI        BbrPI       
BbsI        BciVI       BclI        BfaI        BfrI        
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BsiWI       
BsmI        Bsp1407I    BspEI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CelII       CpoI        DraI        DraIII      
Eam1105I    Eco47III    Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HaeII       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        MaeI        MamI        MfeI        MluI        
MroI        MslI        MunI        NarI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RsrII       SalI        SapI        SbfI        
ScaI        SfoI        SfuI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tsp509I     Tth111I     Van91I      XbaI        
XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AcyI           10  GACGCC               G[GA]^CG[CT]C
AfaI          577  GTAC                 GT^AC
AviII         494  TGCGCA               TGC^GCA
BbvCI         671  CCTCAGC              CCTCAGC(-5/-2)
BcgI          602  GCACTGGCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII         239  AGATCT               A^GATCT
BsaHI          10  GACGCC               G[GA]^CG[CT]C
BseRI         235  GAGGAG               GAGGAG(10/8)
BsiEI         114  CGGCCG               CG[GA][CT]^CG
BsmBI         612  CGTCTC               CGTCTC(1/5)
BspDI         655  ATCGAT               AT^CGAT
BspHI         641  TCATGA               T^CATGA
BstAPI        376  GCATTTGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         239  AGATCT               [GA]^GATC[CT]
ClaI          655  ATCGAT               AT^CGAT
DrdI          811  GACCACCTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          114  CGGCCG               C^GGCCG
EarI          247  CTCTTC               CTCTTC(1/4)
EclXI         114  CGGCCG               C^GGCCG
Eco52I        114  CGGCCG               C^GGCCG
EcoNI         762  CCTCTGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI           22  GCGGG                CCCGC(4/6)
FspI          494  TGCGCA               TGC^GCA
HgaI           10  GACGC                GACGC(5/10)
Hin1I          10  GACGCC               G[GA]^CG[CT]C
Ksp632I       247  CTCTTC               CTCTTC(1/4)
MaeII         407  ACGT                 A^CGT
MflI          239  AGATCT               [GA]^GATC[CT]
NaeI          352  GCCGGC               GCC^GGC
NgoMIV        352  GCCGGC               G^CCGGC
PvuII         863  CAGCTG               CAG^CTG
RcaI          641  TCATGA               T^CATGA
RsaI          577  GTAC                 GT^AC
SacI          146  GAGCTC               GAGCT^C
SexAI         364  ACCAGGT              A^CC[AT]GGT
SfcI          741  CTACAG               C^T[GA][CT]AG
SfiI           37  GGCCCTGGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI          12  CGCCGGTG             C[GA]^CCGG[CT]G
SmlI          304  CTCAAG               C^T[CT][GA]AG
TaqI          656  TCGA                 T^CGA
TfiI          268  GAATC                G^A[AT]TC
TthHB8I       656  TCGA                 T^CGA
XhoII         239  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

ApaI           28  GGGCCC               GGGCC^C
               36  GGGCCC              
AspHI         146  GAGCTC               G[AT]GC[AT]^C
              426  GTGCTC              
AvaI           31  CCCGGG               C^[CT]CG[GA]G
              677  CCCGGG              
BglI           38  GCCCTGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              557  GCCGCTGCGGC         
BpmI          860  CTCCAG               CTGGAG(16/14)
              232  CTGGAG              
BsiHKAI       146  GAGCTC               G[AT]GC[AT]^C
              426  GTGCTC              
BsoBI          31  CCCGGG               C^[CT]CG[GA]G
              677  CCCGGG              
BsrFI          13  GCCGGT               [GA]^CCGG[CT]
              352  GCCGGC              
BstXI         431  CCAAGGAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              461  CCATCATCCTGG        
CfoI          360  GCGC                 GCG^C
              495  GCGC                
Cfr10I         13  GCCGGT               [GA]^CCGG[CT]
              352  GCCGGC              
EcoT14I       431  CCAAGG               C^C[AT][AT]GG
              710  CCAAGG              
HhaI          360  GCGC                 GCG^C
              495  GCGC                
HinP1I        360  GCGC                 G^CGC
              495  GCGC                
MaeIII        187  GTCAC                ^GT[ACGT]AC
              550  GTGAC               
MseI          263  TTAA                 T^TAA
              422  TTAA                
NlaIII        216  CATG                 CATG^
              642  CATG                
PleI          852  GACTC                GAGTC(4/5)
              836  GAGTC               
SmaI           31  CCCGGG               CCC^GGG
              677  CCCGGG              
StyI          431  CCAAGG               C^C[AT][AT]GG
              710  CCAAGG              
Tru9I         263  TTAA                 T^TAA
              422  TTAA                
Tsp45I        187  GTCAC                ^GT[GC]AC
              550  GTGAC               
XmaCI          31  CCCGGG               C^CCGGG
              677  CCCGGG              
XmaI           31  CCCGGG               C^CCGGG
              677  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AccII       AciI        AluI        BalI        BanII       
BbvI        BcnI        BmyI        BsaJI       BsgI        
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EcoO109I    EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        KspI        MboI        MboII       
MluNI       MnlI        MscI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIV       SacII       Sau3AI      Sau96I      ScrFI       
SfaNI       TseI        TspRI       
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