Restriction map for pF1KB3450
 (Circular) (Six-base) MAPSORT of: pF1KB3450.seq  Check: 4486  from: 1  to: 3992



pF1KB3450  3992 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AccI GT'mk_AC

Cuts at:   1030    1030 
   Size:       3992
 
Acc65I G'GTAC_C

Cuts at:   1008    1008 
   Size:       3992
 
AclI AA'CG_TT

Cuts at:   3832    3832 
   Size:       3992
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1817    2249    2996    3264    1817 
   Size:        432     747     268    2545

  Fragments arranged by size: 

                2545     747     432     268
 
AflIII A'CryG_T

Cuts at:   1344    2448    3425    1344 
   Size:       1104     977    1911

  Fragments arranged by size: 

                1911    1104     977
 
AgeI A'CCGG_T

Cuts at:   2380    2380 
   Size:       3992
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    473     473 
   Size:       3992
 
AlwNI CAG_nnn'CTG

Cuts at:   1297    2864    1297 
   Size:       1567    2425
 
ApaI G_GGCC'C

Cuts at:    140     148     140 
   Size:          8    3984
 
ApaLI G'TGCA_C

Cuts at:   2762    2762 
   Size:       3992
 
ApoI r'AATT_y

Cuts at:    996    3345    3601     996 
   Size:       2349     256    1387

  Fragments arranged by size: 

                2349    1387     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3992
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3992
 
AvaI C'yCGr_G

Cuts at:    139     785    1012     139 
   Size:        646     227    3119

  Fragments arranged by size: 

                3119     646     227
 
BamHI G'GATC_C

Cuts at:   1017    1017 
   Size:       3992
 
BanI G'GyrC_C

Cuts at:   1008    1422    1670    1705    1008 
   Size:        414     248      35    3295

  Fragments arranged by size: 

                3295     414     248      35
 
BanII G_rGCy'C

Cuts at:    140     148     258    1006    2036     140 
   Size:          8     110     748    1030    2096

  Fragments arranged by size: 

                2096    1030     748     110       8
 
BbeI G_GCGC'C

Cuts at:   1426    1674    1426 
   Size:        248    3744
 
BbvCI CC'TCA_GC

Cuts at:    780     780 
   Size:       3992
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    699     733     699 
   Size:         34    3958
 
BciVI GTATCCnnnnn_n'

Cuts at:   1890    2651    3992    1890 
   Size:        761    1341    1890

  Fragments arranged by size: 

                1890    1341     761
 
BclI T'GATC_A

Cuts at:   1512    1512 
   Size:       3992
 
BglI GCCn_nnn'nGGC

Cuts at:    152     671     152 
   Size:        519    3473
 
BglII A'GATC_T

Cuts at:    347    1507    3544     347 
   Size:       1160    2037     795

  Fragments arranged by size: 

                2037    1160     795
 
BlpI GC'TnA_GC

Cuts at:   1104    1104 
   Size:       3992
 
Bme1580I G_kGCm'C

Cuts at:    140     148     712    1617    1710    2766     140 
   Size:          8     564     905      93    1056    1366

  Fragments arranged by size: 

                1366    1056     905     564      93       8
 
BmgBI CAC'GTC

Cuts at:    516     516 
   Size:       3992
 
BmrI ACTGGGnnnn_n'

Cuts at:    624    1204    1285    1620    3764     624 
   Size:        580      81     335    2144     852

  Fragments arranged by size: 

                2144     852     580     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2359    2359 
   Size:       3992
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    361     953     361 
   Size:        592    3400
 
Bpu10I CC'TnA_GC

Cuts at:    780     780 
   Size:       3992
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    397    1168    2047    2539    2837     397 
   Size:        771     879     492     298    1552

  Fragments arranged by size: 

                1552     879     771     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3696    3696 
   Size:       3992
 
BsaAI yAC'GTr

Cuts at:   1345    1345 
   Size:       3992
 
BsaBI GATnn'nnATC

Cuts at:      6    1506    3543       6 
   Size:       1500    2037     455

  Fragments arranged by size: 

                2037    1500     455
 
BsaHI Gr'CG_yC

Cuts at:    119    1423    1671    2373    3869     119 
   Size:       1304     248     702    1496     242

  Fragments arranged by size: 

                1496    1304     702     248     242
 
BsaWI w'CCGG_w

Cuts at:   1181    1702    2380    2654    2801    3212    1181 
   Size:        521     678     274     147     411    1961

  Fragments arranged by size: 

                1961     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    882     912     951     981     882 
   Size:         30      39      30    3893

  Fragments arranged by size: 

                3893      39      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    358     358 
   Size:       3992
 
BseYI C'CCAG_C

Cuts at:   1417    2175    2752    3248    1417 
   Size:        758     577     496    2161

  Fragments arranged by size: 

                2161     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    146     463     683     777     973     146 
   Size:        317     220      94     196    3165

  Fragments arranged by size: 

                3165     317     220     196      94
 
BsiEI CG_ry'CG

Cuts at:    104     225    1534    1580    2788     104 
   Size:        121    1309      46    1208    1308

  Fragments arranged by size: 

                1309    1308    1208     121      46
 
BsiHKAI G_wGCw'C

Cuts at:    258     538    1006    1784    1974    2766     258 
   Size:        280     468     778     190     792    1484

  Fragments arranged by size: 

                1484     792     778     468     280     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    726     726 
   Size:       3992
 
Bsp1286I G_dGCh'C

Cuts at:    140     148     258     538     712    1006    1617    1710    1784 
   Size:          8     110     280     174     294     611      93      74

Cuts at:   1784    1974    2036    2766     140 
   Size:        190      62     730    1366

  Fragments arranged by size: 

                1366     730     611     294     280     190     174     110
                  93      74      62       8
 
BspEI T'CCGG_A

Cuts at:   3212    3212 
   Size:       3992
 
BspHI T'CATG_A

Cuts at:    749    3168     749 
   Size:       2419    1573
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1042    1256    1558    1939    1042 
   Size:        214     302     381    3095

  Fragments arranged by size: 

                3095     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2284    2338    3822    3987      50 
   Size:       2234      54    1484     165      55

  Fragments arranged by size: 

                2234    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1904    1904 
   Size:       3992
 
BsrFI r'CCGG_y

Cuts at:    121     460    1990    2171    2380     121 
   Size:        339    1530     181     209    1733

  Fragments arranged by size: 

                1733    1530     339     209     181
 
BssSI C'ACGA_G

Cuts at:   2263    2621    2263 
   Size:        358    3634
 
BstAPI GCAn_nnn'nTGC

Cuts at:    490    1369     490 
   Size:        879    3113
 
BstBI TT'CG_AA

Cuts at:   2353    2353 
   Size:       3992
 
BstXI CCAn_nnnn'nTGG

Cuts at:    546     576     546 
   Size:         30    3962
 
BstYI r'GATC_y

Cuts at:      1     347    1017    1484    1507    1842    2088    3100    3186 
   Size:        346     670     467      23     335     246    1012      86

Cuts at:   3186    3198    3544       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     670     467     449     346     346     335     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     171     183     225     249     561     107 
   Size:         64      12      42      24     312    3538

  Fragments arranged by size: 

                3538     312      64      42      24      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1942    2123    2286    1942 
   Size:        181     163    3648

  Fragments arranged by size: 

                3648     181     163
 
BtsI GCAGTG_nn'

Cuts at:    204     794    1235     204 
   Size:        590     441    2961

  Fragments arranged by size: 

                2961     590     441
 
ClaI AT'CG_AT

Cuts at:    764     764 
   Size:       3992
 
DraI TTT'AAA

Cuts at:    991     991 
   Size:       3992
 
DrdI GACnn_nn'nnGTC

Cuts at:    925    1698    2556     925 
   Size:        773     858    2361

  Fragments arranged by size: 

                2361     858     773
 
EaeI y'GGCC_r

Cuts at:    222     462     510     548     578    1577    1751    2142    2169 
   Size:        240      48      38      30     999     174     391      27

Cuts at:   2169    3514     222 
   Size:       1345     700

  Fragments arranged by size: 

                1345     999     700     391     240     174      48      38
                  30      27
 
EagI C'GGCC_G

Cuts at:    222    1577     222 
   Size:       1355    2637
 
EarI CTCTTCn'nnn_

Cuts at:    361    2015    2225     361 
   Size:       1654     210    2128

  Fragments arranged by size: 

                2128    1654     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    127    2200    2510    2656    3802     127 
   Size:       2073     310     146    1146     317

  Fragments arranged by size: 

                2073    1146     317     310     146
 
EcoICRI GAG'CTC

Cuts at:    256    1004     256 
   Size:        748    3244
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    361     953    1817    2249    2996    3264     361 
   Size:        592     864     432     747     268    1089

  Fragments arranged by size: 

                1089     864     747     592     432     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:    874     874 
   Size:       3992
 
EcoO109I rG'GnC_Cy

Cuts at:    136     144     145     404    1131     136 
   Size:          8       1     259     727    2997

  Fragments arranged by size: 

                2997     727     259       8       1
 
EcoRI G'AATT_C

Cuts at:    996     996 
   Size:       3992
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    843    2011     843 
   Size:       1168    2824
 
FspI TGC'GCA

Cuts at:    604    1773     604 
   Size:       1169    2823
 
HaeII r_GCGC'y

Cuts at:   1426    1674    2696    3675    1426 
   Size:        248    1022     979    1743

  Fragments arranged by size: 

                1743    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    951     983    1834    1864    1866    1896     951 
   Size:         32     851      30       2      30    3047

  Fragments arranged by size: 

                3047     851      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1031    1031 
   Size:       3992
 
KasI G'GCGC_C

Cuts at:   1422    1670    1422 
   Size:        248    3744
 
KpnI G_GTAC'C

Cuts at:   1012    1012 
   Size:       3992
 
MluI A'CGCG_T

Cuts at:   3425    3425 
   Size:       3992
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2663    2847    2663 
   Size:        184    3808
 
MscI TGG'CCA

Cuts at:    512     550     580    1753     512 
   Size:         38      30    1173    2751

  Fragments arranged by size: 

                2751    1173      38      30
 
MslI CAynn'nnrTG

Cuts at:   1981    2108    1981 
   Size:        127    3865
 
MspA1I CmG'CkG

Cuts at:    130     173     185     251     668     957     973    1103    1417 
   Size:         43      12      66     417     289      16     130     314

Cuts at:   1417    1777    2790    3035     130 
   Size:        360    1013     245    1087

  Fragments arranged by size: 

                1087    1013     417     360     314     289     245     130
                  66      43      16      12
 
NaeI GCC'GGC

Cuts at:    462    2173     462 
   Size:       1711    2281
 
NarI GG'CG_CC

Cuts at:   1423    1671    1423 
   Size:        248    3744
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3992
 
NgoMIV G'CCGG_C

Cuts at:    460    2171     460 
   Size:       1711    2281
 
NspI r_CATG'y

Cuts at:   1045    1545    2452    1045 
   Size:        500     907    2585

  Fragments arranged by size: 

                2585     907     500
 
PasI CC'CwG_GG

Cuts at:    157     704     804     157 
   Size:        547     100    3345

  Fragments arranged by size: 

                3345     547     100
 
PciI A'CATG_T

Cuts at:   2448    2448 
   Size:       3992
 
PmeI GTTT'AAAC

Cuts at:    991     991 
   Size:       3992
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3131    3163    3131 
   Size:         32    3960
 
PspOMI G'GGCC_C

Cuts at:    136     144     136 
   Size:          8    3984
 
PstI C_TGCA'G

Cuts at:   1039    1724    1039 
   Size:        685    3307
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3992
 
PvuII CAG'CTG

Cuts at:    973    1417    1777     973 
   Size:        444     360    3188

  Fragments arranged by size: 

                3188     444     360
 
RsrII CG'GwC_CG

Cuts at:   2187    2187 
   Size:       3992
 
SacI G_AGCT'C

Cuts at:    258    1006     258 
   Size:        748    3244
 
SacII CC_GC'GG

Cuts at:    174     186     252     174 
   Size:         12      66    3914

  Fragments arranged by size: 

                3914      66      12
 
SalI G'TCGA_C

Cuts at:   1029    1029 
   Size:       3992
 
SapI GCTCTTCn'nnn_

Cuts at:   2015    2225    2015 
   Size:        210    3782
 
SbfI CC_TGCA'GG

Cuts at:   1039    1039 
   Size:       3992
 
ScaI AGT'ACT

Cuts at:   3509    3509 
   Size:       3992
 
SexAI A'CCwGG_T

Cuts at:    472     472 
   Size:       3992
 
SfcI C'TryA_G

Cuts at:     33     849    1035    1720    2713    2904      33 
   Size:        816     186     685     993     191    1121

  Fragments arranged by size: 

                1121     993     816     685     191     186
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    152     152 
   Size:       3992
 
SfoI GGC'GCC

Cuts at:   1424    1672    1424 
   Size:        248    3744
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3992
 
SgrAI Cr'CCGG_yG

Cuts at:    121     121 
   Size:       3992
 
SmaI CCC'GGG

Cuts at:    141     787    1014     141 
   Size:        646     227    3119

  Fragments arranged by size: 

                3119     646     227
 
SmlI C'TyrA_G

Cuts at:    412    1147    2062    2554    2816     412 
   Size:        735     915     492     262    1588

  Fragments arranged by size: 

                1588     915     735     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3992
 
SphI G_CATG'C

Cuts at:   1045    1545    1045 
   Size:        500    3492
 
StyI C'CwwG_G

Cuts at:    107     539     818    1126    1480     107 
   Size:        432     279     308     354    2619

  Fragments arranged by size: 

                2619     432     354     308     279
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    508     514     717    1575    1708    2167    2376     508 
   Size:          6     203     858     133     459     209    2124

  Fragments arranged by size: 

                2124     858     459     209     203     133       6
 
TatI w'GTAC_w

Cuts at:   3507    3507 
   Size:       3992
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    159     191     268     300     882     914    3509    3541     159 
   Size:         32      77      32     582      32    2595      32     610

  Fragments arranged by size: 

                2595     610     582      77      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   1789    1789 
   Size:       3992
 
XbaI T'CTAG_A

Cuts at:   1023    1023 
   Size:       3992
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1487    1487 
   Size:       3992
 
XmaI C'CCGG_G

Cuts at:    139     785    1012     139 
   Size:        646     227    3119

  Fragments arranged by size: 

                3119     646     227
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI
     BtgI    BtgZI     BtsI     ClaI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI     PciI     PmeI     PpiI   PspOMI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI     SpeI
     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AloI     AscI
    AvrII     BaeI     BbsI    BfrBI     BmtI    BsiWI     BsmI    BsrGI
   BssHII   BstEII  BstZ17I   Bsu36I    CspCI   DraIII    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI
     NsiI     PacI    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI
    PspXI     PsrI    SanDI    SnaBI     SrfI     SspI     StuI     SwaI
     XhoI     XmnI     ZraI
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