Restriction map for pF1KB3432
##################################
# NO CUT ENZYMES (  184 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BanI        BbeI        BbrPI       
BbsI        BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrGI       BsrI        BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstF5I      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FokI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HpaI        KasI        KpnI        Ksp632I     
KspI        MaeI        MamI        MfeI        MflI        
MluI        MroI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        Tsp509I     
Tth111I     TthHB8I     XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AlwNI         209  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI          16  GAGCTC               G[AT]GC[AT]^C
BalI          152  TGGCCA               TGG^CCA
BanII          16  GAGCTC               G[GA]GC[CT]^C
BbvCI         265  GCTGAGG              CCTCAGC(-5/-2)
BglI          120  GCCTTTTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI           16  GAGCTC               G[AGT]GC[ACT]^C
BsiHKAI        16  GAGCTC               G[AT]GC[AT]^C
BsmAI          10  GTCTC                GTCTC(1/5)
Bsp1286I       16  GAGCTC               G[AGT]GC[ACT]^C
BspMI         201  ACCTGC               ACCTGC(4/8)
BsrDI         182  GCAATG               GCAATG(2/0)
BstAPI        211  GCAGCTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DdeI          266  CTGAG                C^T[ACGT]AG
DpnII          81  GATC                 ^GATC
EaeI          152  TGGCCA               [CT]^GGCC[GA]
HincII        160  GTCAAC               GT[CT]^[GA]AC
HindII        160  GTCAAC               GT[CT]^[GA]AC
HphI           60  GGTGA                GGTGA(8/7)
MaeII         158  ACGT                 A^CGT
MboI           81  GATC                 ^GATC
MluNI         152  TGGCCA               TGG^CCA
MscI          152  TGGCCA               TGG^CCA
MseI           95  TTAA                 T^TAA
MslI           49  CACGACGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII          81  GATC                 ^GATC
NlaIII        321  CATG                 CATG^
PflMI         208  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          307  GAGTC                GAGTC(4/5)
PpuMI         234  AGGTCCT              [GA]G^G[AT]CC[CT]
SacI           16  GAGCTC               GAGCT^C
Sau3AI         81  GATC                 ^GATC
SfaNI          26  GCATC                GCATC(5/9)
TfiI          298  GAATC                G^A[AT]TC
Tru9I          95  TTAA                 T^TAA
TspRI         249  CACTG                CA[GC]TG[ACGT][ACGT]^
Van91I        208  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AvaII         199  GGACC                G^G[AT]CC
              235  GGTCC               
BpmI          206  CTCCAG               CTGGAG(16/14)
              260  CTCCAG              
BsaJI         149  CCCTGG               C^C[ACGT][ACGT]GG
              302  CCGAGG              
BseRI         268  GAGGAG               GAGGAG(10/8)
              304  GAGGAG              
BsgI           46  CTGCAC               GTGCAG(16/14)
              221  CTGCAC              
BsiYI         208  CCAGCAGCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              226  CCAGCAGGAGG         
BslI          208  CCAGCAGCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              226  CCAGCAGGAGG         
BsrFI         103  GCCGGT               [GA]^CCGG[CT]
              193  GCCGGT              
BssKI         130  CCTGG                ^CC[ACGT]GG
              150  CCTGG               
BstNI         130  CCTGG                CC^[AT]GG
              150  CCTGG               
Cac8I          18  GCTCGC               GC[ACGT]^[ACGT]GC
               22  GCCTGC              
Cfr10I        103  GCCGGT               [GA]^CCGG[CT]
              193  GCCGGT              
DraII         146  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              234  AGGTCCT             
EcoO109I      146  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              234  AGGTCCT             
EcoRII        130  CCTGG                ^CC[AT]GG
              150  CCTGG               
HapII         104  CCGG                 C^CGG
              194  CCGG                
HinfI         298  GAATC                G^A[ACGT]TC
              307  GAGTC               
HpaII         104  CCGG                 C^CGG
              194  CCGG                
MaeIII         61  GTGAC                ^GT[ACGT]AC
               67  GTCAC               
MboII         273  GAAGA                GAAGA(8/7)
              295  GAAGA               
MspA1I        212  CAGCTG               C[AC]G^C[GT]G
              263  CAGCTG              
MspI          104  CCGG                 C^CGG
              194  CCGG                
MvaI          130  CCTGG                CC^[AT]GG
              150  CCTGG               
NlaIV         170  GGAGCC               GG[ACGT]^[ACGT]CC
              190  GGGGCC              
PvuII         212  CAGCTG               CAG^CTG
              263  CAGCTG              
ScrFI         130  CCTGG                CC^[ACGT]GG
              150  CCTGG               
Tsp45I         61  GTGAC                ^GT[GC]AC
               67  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   10 enzymes) #
#########################################

AluI        BbvI        Cfr13I      Fnu4HI      HaeIII      
ItaI        MnlI        MwoI        Sau96I      TseI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com