Restriction map for pF1KB3432
 (Circular) (Six-base) MAPSORT of: pF1KB3432.seq  Check: 526  from: 1  to: 3455



pF1KB3432  3455 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AccI GT'mk_AC

Cuts at:    493     493 
   Size:       3455
 
Acc65I G'GTAC_C

Cuts at:    471     471 
   Size:       3455
 
AclI AA'CG_TT

Cuts at:   3295    3295 
   Size:       3455
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1280    1712    2459    2727    1280 
   Size:        432     747     268    2008

  Fragments arranged by size: 

                2008     747     432     268
 
AflIII A'CryG_T

Cuts at:    807    1911    2888     807 
   Size:       1104     977    1374

  Fragments arranged by size: 

                1374    1104     977
 
AgeI A'CCGG_T

Cuts at:   1843    1843 
   Size:       3455
 
AlwNI CAG_nnn'CTG

Cuts at:    322     760    2327     322 
   Size:        438    1567    1450

  Fragments arranged by size: 

                1567    1450     438
 
ApaLI G'TGCA_C

Cuts at:   2225    2225 
   Size:       3455
 
ApoI r'AATT_y

Cuts at:    459    2808    3064     459 
   Size:       2349     256     850

  Fragments arranged by size: 

                2349     850     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3455
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3455
 
AvaI C'yCGr_G

Cuts at:    475     475 
   Size:       3455
 
BamHI G'GATC_C

Cuts at:    480     480 
   Size:       3455
 
BanI G'GyrC_C

Cuts at:    471     885    1133    1168     471 
   Size:        414     248      35    2758

  Fragments arranged by size: 

                2758     414     248      35
 
BanII G_rGCy'C

Cuts at:    128     469    1499     128 
   Size:        341    1030    2084

  Fragments arranged by size: 

                2084    1030     341
 
BbeI G_GCGC'C

Cuts at:    889    1137     889 
   Size:        248    3207
 
BbvCI CC'TCA_GC

Cuts at:    374     374 
   Size:       3455
 
BciVI GTATCCnnnnn_n'

Cuts at:   1353    2114    3455    1353 
   Size:        761    1341    1353

  Fragments arranged by size: 

                1353    1341     761
 
BclI T'GATC_A

Cuts at:    975     975 
   Size:       3455
 
BglI GCCn_nnn'nGGC

Cuts at:    234     234 
   Size:       3455
 
BglII A'GATC_T

Cuts at:    970    3007     970 
   Size:       2037    1418
 
BlpI GC'TnA_GC

Cuts at:    567     567 
   Size:       3455
 
Bme1580I G_kGCm'C

Cuts at:   1080    1173    2229    1080 
   Size:         93    1056    2306

  Fragments arranged by size: 

                2306    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    667     748    1083    3227     667 
   Size:         81     335    2144     895

  Fragments arranged by size: 

                2144     895     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1822    1822 
   Size:       3455
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    299     353     299 
   Size:         54    3401
 
Bpu10I CC'TnA_GC

Cuts at:    374     374 
   Size:       3455
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    631    1510    2002    2300     631 
   Size:        879     492     298    1786

  Fragments arranged by size: 

                1786     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3159    3159 
   Size:       3455
 
BsaAI yAC'GTr

Cuts at:    808     808 
   Size:       3455
 
BsaBI GATnn'nnATC

Cuts at:      6     969    3006       6 
   Size:        963    2037     455

  Fragments arranged by size: 

                2037     963     455
 
BsaHI Gr'CG_yC

Cuts at:    886    1134    1836    3332     886 
   Size:        248     702    1496    1009

  Fragments arranged by size: 

                1496    1009     702     248
 
BsaWI w'CCGG_w

Cuts at:    644    1165    1843    2117    2264    2675     644 
   Size:        521     678     274     147     411    1424

  Fragments arranged by size: 

                1424     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    391     427     391 
   Size:         36    3419
 
BseYI C'CCAG_C

Cuts at:    880    1638    2215    2711     880 
   Size:        758     577     496    1624

  Fragments arranged by size: 

                1624     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    139     314     139 
   Size:        175    3280
 
BsiEI CG_ry'CG

Cuts at:    104     997    1043    2251     104 
   Size:        893      46    1208    1308

  Fragments arranged by size: 

                1308    1208     893      46
 
BsiHKAI G_wGCw'C

Cuts at:    128     469    1247    1437    2229     128 
   Size:        341     778     190     792    1354

  Fragments arranged by size: 

                1354     792     778     341     190
 
Bsp1286I G_dGCh'C

Cuts at:    128     469    1080    1173    1247    1437    1499    2229     128 
   Size:        341     611      93      74     190      62     730    1354

  Fragments arranged by size: 

                1354     730     611     341     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2675    2675 
   Size:       3455
 
BspHI T'CATG_A

Cuts at:   2631    2631 
   Size:       3455
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    318     505     719    1021    1402     318 
   Size:        187     214     302     381    2371

  Fragments arranged by size: 

                2371     381     302     214     187
 
BsrBI CCG'CTC

Cuts at:     50    1747    1801    3285    3450      50 
   Size:       1697      54    1484     165      55

  Fragments arranged by size: 

                1697    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    297    1367     297 
   Size:       1070    2385
 
BsrFI r'CCGG_y

Cuts at:    211     301    1453    1634    1843     211 
   Size:         90    1152     181     209    1823

  Fragments arranged by size: 

                1823    1152     209     181      90
 
BssSI C'ACGA_G

Cuts at:   1726    2084    1726 
   Size:        358    3097
 
BstAPI GCAn_nnn'nTGC

Cuts at:    325     832     325 
   Size:        507    2948
 
BstBI TT'CG_AA

Cuts at:   1816    1816 
   Size:       3455
 
BstYI r'GATC_y

Cuts at:      1     480     947     970    1305    1551    2563    2649    2661 
   Size:        479     467      23     335     246    1012      86      12

Cuts at:   2661    3007       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     479     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3455
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1405    1586    1749    1405 
   Size:        181     163    3111

  Fragments arranged by size: 

                3111     181     163
 
BtsI GCAGTG_nn'

Cuts at:    356     698     356 
   Size:        342    3113
 
DraI TTT'AAA

Cuts at:    454     454 
   Size:       3455
 
DrdI GACnn_nn'nnGTC

Cuts at:   1161    2019    1161 
   Size:        858    2597
 
EaeI y'GGCC_r

Cuts at:    260    1040    1214    1605    1632    2977     260 
   Size:        780     174     391      27    1345     738

  Fragments arranged by size: 

                1345     780     738     391     174      27
 
EagI C'GGCC_G

Cuts at:   1040    1040 
   Size:       3455
 
EarI CTCTTCn'nnn_

Cuts at:   1478    1688    1478 
   Size:        210    3245
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1663    1973    2119    3265    1663 
   Size:        310     146    1146    1853

  Fragments arranged by size: 

                1853    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    126     467     126 
   Size:        341    3114
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    299     353    1280    1712    2459    2727     299 
   Size:         54     927     432     747     268    1027

  Fragments arranged by size: 

                1027     927     747     432     268      54
 
EcoO109I rG'GnC_Cy

Cuts at:    255     343     594     255 
   Size:         88     251    3116

  Fragments arranged by size: 

                3116     251      88
 
EcoRI G'AATT_C

Cuts at:    459     459 
   Size:       3455
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1474    1474 
   Size:       3455
 
FspI TGC'GCA

Cuts at:   1236    1236 
   Size:       3455
 
HaeII r_GCGC'y

Cuts at:    889    1137    2159    3138     889 
   Size:        248    1022     979    1206

  Fragments arranged by size: 

                1206    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1297    1327    1329    1359    1297 
   Size:         30       2      30    3393

  Fragments arranged by size: 

                3393      30      30       2
 
HincII GTy'rAC

Cuts at:    270     494     270 
   Size:        224    3231
 
KasI G'GCGC_C

Cuts at:    885    1133     885 
   Size:        248    3207
 
KpnI G_GTAC'C

Cuts at:    475     475 
   Size:       3455
 
MluI A'CGCG_T

Cuts at:   2888    2888 
   Size:       3455
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2126    2310    2126 
   Size:        184    3271
 
MscI TGG'CCA

Cuts at:    262    1216     262 
   Size:        954    2501
 
MslI CAynn'nnrTG

Cuts at:    161    1444    1571     161 
   Size:       1283     127    2045

  Fragments arranged by size: 

                2045    1283     127
 
MspA1I CmG'CkG

Cuts at:    322     373     566     880    1240    2253    2498     322 
   Size:         51     193     314     360    1013     245    1279

  Fragments arranged by size: 

                1279    1013     360     314     245     193      51
 
NaeI GCC'GGC

Cuts at:   1636    1636 
   Size:       3455
 
NarI GG'CG_CC

Cuts at:    886    1134     886 
   Size:        248    3207
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3455
 
NgoMIV G'CCGG_C

Cuts at:   1634    1634 
   Size:       3455
 
NspI r_CATG'y

Cuts at:    508    1008    1915     508 
   Size:        500     907    2048

  Fragments arranged by size: 

                2048     907     500
 
PciI A'CATG_T

Cuts at:   1911    1911 
   Size:       3455
 
PflMI CCAn_nnn'nTGG

Cuts at:    322     322 
   Size:       3455
 
PmeI GTTT'AAAC

Cuts at:    454     454 
   Size:       3455
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2594    2626    2594 
   Size:         32    3423
 
PpuMI rG'GwC_Cy

Cuts at:    343     343 
   Size:       3455
 
PstI C_TGCA'G

Cuts at:    502    1187     502 
   Size:        685    2770
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3455
 
PvuII CAG'CTG

Cuts at:    322     373     880    1240     322 
   Size:         51     507     360    2537

  Fragments arranged by size: 

                2537     507     360      51
 
RsrII CG'GwC_CG

Cuts at:   1650    1650 
   Size:       3455
 
SacI G_AGCT'C

Cuts at:    128     469     128 
   Size:        341    3114
 
SalI G'TCGA_C

Cuts at:    492     492 
   Size:       3455
 
SapI GCTCTTCn'nnn_

Cuts at:   1478    1688    1478 
   Size:        210    3245
 
SbfI CC_TGCA'GG

Cuts at:    502     502 
   Size:       3455
 
ScaI AGT'ACT

Cuts at:   2972    2972 
   Size:       3455
 
SfcI C'TryA_G

Cuts at:     33     498    1183    2176    2367      33 
   Size:        465     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     465     191
 
SfoI GGC'GCC

Cuts at:    887    1135     887 
   Size:        248    3207
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3455
 
SmaI CCC'GGG

Cuts at:    477     477 
   Size:       3455
 
SmlI C'TyrA_G

Cuts at:    610    1525    2017    2279     610 
   Size:        915     492     262    1786

  Fragments arranged by size: 

                1786     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3455
 
SphI G_CATG'C

Cuts at:    508    1008     508 
   Size:        500    2955
 
StyI C'CwwG_G

Cuts at:    107     589     943     107 
   Size:        482     354    2619

  Fragments arranged by size: 

                2619     482     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1038    1171    1630    1839    1038 
   Size:        133     459     209    2654

  Fragments arranged by size: 

                2654     459     209     133
 
TatI w'GTAC_w

Cuts at:   2970    2970 
   Size:       3455
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2972    3004    2972 
   Size:         32    3423
 
Tth111I GACn'n_nGTC

Cuts at:   1252    1252 
   Size:       3455
 
XbaI T'CTAG_A

Cuts at:    486     486 
   Size:       3455
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    950     950 
   Size:       3455
 
XmaI C'CCGG_G

Cuts at:    475     475 
   Size:       3455
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BbvCI    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI
    PflMI     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI     BcgI    BfrBI    BmgBI
     BmtI    BsaXI    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII
    BstXI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PfoI     PmlI    PshAI     PsiI
   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI
    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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