Restriction map for pF1KB3443
##################################
# NO CUT ENZYMES (  178 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AciI        
AclI        AcyI        AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BbeI        
BbrPI       BciVI       BclI        BcnI        BfaI        
BglI        BlnI        BlpI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       BspDI       BspEI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrI        BssHII      
BssKI       Bst1107I    BstEII      BstNI       BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraII       
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FseI        
FspI        HapII       HgaI        Hin1I       HincII      
HindII      HpaI        HpaII       HphI        KasI        
KspI        MaeI        MaeII       MaeIII      MamI        
MfeI        MluI        MluNI       MroI        MscI        
MspA1I      MspI        MunI        MvaI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
PacI        PaeR7I      PflFI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        ScrFI       SexAI       SfiI        SfoI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        StyI        SwaI        
Tsp45I      TspRI       Tth111I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

Acc65I        428  GGTACC               G^GTACC
AflII          52  CTTAAG               C^TTAAG
Asp718I       428  GGTACC               G^GTACC
AvaII         711  GGACC                G^G[AT]CC
BanI          428  GGTACC               G^G[CT][GA]CC
BanII         303  GAGCCC               G[GA]GC[CT]^C
BbsI          639  GTCTTC               GAAGAC(2/6)
BbvCI         396  GCTGAGG              CCTCAGC(-5/-2)
BbvI          327  GCAGC                GCAGC(8/12)
BfrI           52  CTTAAG               C^TTAAG
BglII         578  AGATCT               A^GATCT
BmyI          303  GAGCCC               G[AGT]GC[ACT]^C
BpuAI         639  GTCTTC               GAAGAC(2/6)
BsiYI         646  CCACGAGGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          646  CCACGAGGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I      303  GAGCCC               G[AGT]GC[ACT]^C
Bsp1407I      191  TGTACA               T^GTACA
BspHI         573  TCATGA               T^CATGA
BspMI         249  ACCTGC               ACCTGC(4/8)
BsrGI         191  TGTACA               T^GTACA
BssSI         647  CACGAG               CACGAG(-5/-1)
BstAPI        548  GCATCATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        218  GGATG                GGATG(2/0)
BstYI         578  AGATCT               [GA]^GATC[CT]
CfoI           93  GCGC                 GCG^C
DraIII        647  CACGAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI           13  GAAGAG               CTCTTC(1/4)
Fnu4HI        327  GCAGC                GC^[ACGT]GC
FokI          218  GGATG                GGATG(9/13)
HaeII          92  AGCGCC               [GA]GCGC^[CT]
HaeIII        626  GGCC                 GG^CC
HhaI           93  GCGC                 GCG^C
HinP1I         93  GCGC                 G^CGC
HindIII       142  AAGCTT               A^AGCTT
ItaI          327  GCAGC                GC^[ACGT]GC
KpnI          428  GGTACC               GGTAC^C
Ksp632I        13  GAAGAG               CTCTTC(1/4)
MflI          578  AGATCT               [GA]^GATC[CT]
MslI          552  CATCTGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI          392  ACATGC               [GA]CATG^[CT]
PflMI         646  CCACGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          251  CTGCAG               CTGCA^G
RcaI          573  TCATGA               T^CATGA
SfcI          251  CTGCAG               C^T[GA][CT]AG
SmlI           52  CTTAAG               C^T[CT][GA]AG
SspBI         191  TGTACA               T^GTACA
TseI          327  GCAGC                G^C[AT]GC
Van91I        646  CCACGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          412  CCATATATTGCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         578  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AcsI           69  GAATTT               [GA]^AATT[CT]
              203  AAATTC              
ApoI           69  GAATTT               [GA]^AATT[CT]
              203  AAATTC              
BcgI          386  CGATTAACATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              327  GCAGCTTCTTCG        
Cac8I         232  GCTGGC               GC[ACGT]^[ACGT]GC
              697  GCTGGC              
Cfr13I        626  GGCCC                G^G[ACGT]CC
              711  GGACC               
DdeI           97  CTGAG                C^T[ACGT]AG
              397  CTGAG               
HinfI         281  GAATC                G^A[ACGT]TC
              570  GATTC               
MseI           53  TTAA                 T^TAA
              389  TTAA                
Sau96I        626  GGCCC                G^G[ACGT]CC
              711  GGACC               
TfiI          281  GAATC                G^A[AT]TC
              570  GATTC               
Tru9I          53  TTAA                 T^TAA
              389  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   19 enzymes) #
#########################################

AfaI        AluI        BstBI       DpnII       MboI        
MboII       MnlI        MwoI        NdeII       NlaIII      
NlaIV       NspV        RsaI        Sau3AI      SfaNI       
SfuI        TaqI        Tsp509I     TthHB8I     
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