Restriction map for pF1KB3443
 (Circular) (Six-base) MAPSORT of: pF1KB3443.seq  Check: 2788  from: 1  to: 3857



pF1KB3443  3857 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AccI GT'mk_AC

Cuts at:    895     895 
   Size:       3857
 
Acc65I G'GTAC_C

Cuts at:    536     873     536 
   Size:        337    3520
 
AclI AA'CG_TT

Cuts at:    851    3697     851 
   Size:       2846    1011
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    211     568    1682    2114    2861    3129     211 
   Size:        357    1114     432     747     268     939

  Fragments arranged by size: 

                1114     939     747     432     357     268
 
AflII C'TTAA_G

Cuts at:    160     160 
   Size:       3857
 
AflIII A'CryG_T

Cuts at:   1209    2313    3290    1209 
   Size:       1104     977    1776

  Fragments arranged by size: 

                1776    1104     977
 
AgeI A'CCGG_T

Cuts at:   2245    2245 
   Size:       3857
 
AlwNI CAG_nnn'CTG

Cuts at:   1162    2729    1162 
   Size:       1567    2290
 
ApaLI G'TGCA_C

Cuts at:   2627    2627 
   Size:       3857
 
ApoI r'AATT_y

Cuts at:    177     311     861    3210    3466     177 
   Size:        134     550    2349     256     568

  Fragments arranged by size: 

                2349     568     550     256     134
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3857
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3857
 
AvaI C'yCGr_G

Cuts at:    877     877 
   Size:       3857
 
BamHI G'GATC_C

Cuts at:    882     882 
   Size:       3857
 
BanI G'GyrC_C

Cuts at:    536     873    1287    1535    1570     536 
   Size:        337     414     248      35    2823

  Fragments arranged by size: 

                2823     414     337     248      35
 
BanII G_rGCy'C

Cuts at:    415     871    1901     415 
   Size:        456    1030    2371

  Fragments arranged by size: 

                2371    1030     456
 
BbeI G_GCGC'C

Cuts at:   1291    1539    1291 
   Size:        248    3609
 
BbsI GAAGACnn'nnnn_

Cuts at:    740     740 
   Size:       3857
 
BbvCI CC'TCA_GC

Cuts at:    505     505 
   Size:       3857
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    424     458     483     517     424 
   Size:         34      25      34    3764

  Fragments arranged by size: 

                3764      34      34      25
 
BciVI GTATCCnnnnn_n'

Cuts at:   1755    2516    3857    1755 
   Size:        761    1341    1755

  Fragments arranged by size: 

                1755    1341     761
 
BclI T'GATC_A

Cuts at:   1377    1377 
   Size:       3857
 
BglII A'GATC_T

Cuts at:    686    1372    3409     686 
   Size:        686    2037    1134

  Fragments arranged by size: 

                2037    1134     686
 
BlpI GC'TnA_GC

Cuts at:    969     969 
   Size:       3857
 
Bme1580I G_kGCm'C

Cuts at:   1482    1575    2631    1482 
   Size:         93    1056    2708

  Fragments arranged by size: 

                2708    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1069    1150    1485    3629    1069 
   Size:         81     335    2144    1297

  Fragments arranged by size: 

                2144    1297     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2224    2224 
   Size:       3857
 
Bpu10I CC'TnA_GC

Cuts at:    505     505 
   Size:       3857
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1033    1912    2404    2702    1033 
   Size:        879     492     298    2188

  Fragments arranged by size: 

                2188     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3561    3561 
   Size:       3857
 
BsaAI yAC'GTr

Cuts at:   1210    1210 
   Size:       3857
 
BsaBI GATnn'nnATC

Cuts at:      6    1371    3408       6 
   Size:       1365    2037     455

  Fragments arranged by size: 

                2037    1365     455
 
BsaHI Gr'CG_yC

Cuts at:   1288    1536    2238    3734    1288 
   Size:        248     702    1496    1411

  Fragments arranged by size: 

                1496    1411     702     248
 
BsaWI w'CCGG_w

Cuts at:   1046    1567    2245    2519    2666    3077    1046 
   Size:        521     678     274     147     411    1826

  Fragments arranged by size: 

                1826     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:   1282    2040    2617    3113    1282 
   Size:        758     577     496    2026

  Fragments arranged by size: 

                2026     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1399    1445    2653     104 
   Size:       1295      46    1208    1308

  Fragments arranged by size: 

                1308    1295    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    871    1649    1839    2631     871 
   Size:        778     190     792    2097

  Fragments arranged by size: 

                2097     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    415     871    1482    1575    1649    1839    1901    2631     415 
   Size:        456     611      93      74     190      62     730    1641

  Fragments arranged by size: 

                1641     730     611     456     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   3077    3077 
   Size:       3857
 
BspHI T'CATG_A

Cuts at:    681    3033     681 
   Size:       2352    1505
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    366     907    1121    1423    1804     366 
   Size:        541     214     302     381    2419

  Fragments arranged by size: 

                2419     541     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2149    2203    3687    3852      50 
   Size:       2099      54    1484     165      55

  Fragments arranged by size: 

                2099    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1769    1769 
   Size:       3857
 
BsrFI r'CCGG_y

Cuts at:   1855    2036    2245    1855 
   Size:        181     209    3467

  Fragments arranged by size: 

                3467     209     181
 
BsrGI T'GTAC_A

Cuts at:    299     299 
   Size:       3857
 
BssSI C'ACGA_G

Cuts at:    755    2128    2486     755 
   Size:       1373     358    2126

  Fragments arranged by size: 

                2126    1373     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    662    1234     662 
   Size:        572    3285
 
BstBI TT'CG_AA

Cuts at:    234     315     423    2218     234 
   Size:         81     108    1795    1873

  Fragments arranged by size: 

                1873    1795     108      81
 
BstYI r'GATC_y

Cuts at:      1     686     882    1349    1372    1707    1953    2965    3051 
   Size:        685     196     467      23     335     246    1012      86

Cuts at:   3051    3063    3409       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     685     467     449     346     335     246     196
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3857
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1807    1988    2151    1807 
   Size:        181     163    3513

  Fragments arranged by size: 

                3513     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1100    1100 
   Size:       3857
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    768     803     768 
   Size:         35    3822
 
DraI TTT'AAA

Cuts at:    856     856 
   Size:       3857
 
DraIII CAC_nnn'GTG

Cuts at:    760     760 
   Size:       3857
 
DrdI GACnn_nn'nnGTC

Cuts at:   1563    2421    1563 
   Size:        858    2999
 
EaeI y'GGCC_r

Cuts at:   1442    1616    2007    2034    3379    1442 
   Size:        174     391      27    1345    1920

  Fragments arranged by size: 

                1920    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1442    1442 
   Size:       3857
 
EarI CTCTTCn'nnn_

Cuts at:    116    1880    2090     116 
   Size:       1764     210    1883

  Fragments arranged by size: 

                1883    1764     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2065    2375    2521    3667    2065 
   Size:        310     146    1146    2255

  Fragments arranged by size: 

                2255    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    869     869 
   Size:       3857
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    211     568    1682    2114    2861    3129     211 
   Size:        357    1114     432     747     268     939

  Fragments arranged by size: 

                1114     939     747     432     357     268
 
EcoO109I rG'GnC_Cy

Cuts at:    996     996 
   Size:       3857
 
EcoRI G'AATT_C

Cuts at:    861     861 
   Size:       3857
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1876    1876 
   Size:       3857
 
FspI TGC'GCA

Cuts at:   1638    1638 
   Size:       3857
 
HaeII r_GCGC'y

Cuts at:    204    1291    1539    2561    3540     204 
   Size:       1087     248    1022     979     521

  Fragments arranged by size: 

                1087    1022     979     521     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1699    1729    1731    1761    1699 
   Size:         30       2      30    3795

  Fragments arranged by size: 

                3795      30      30       2
 
HincII GTy'rAC

Cuts at:    896     896 
   Size:       3857
 
HindIII A'AGCT_T

Cuts at:    250     250 
   Size:       3857
 
KasI G'GCGC_C

Cuts at:   1287    1535    1287 
   Size:        248    3609
 
KpnI G_GTAC'C

Cuts at:    540     877     540 
   Size:        337    3520
 
MluI A'CGCG_T

Cuts at:   3290    3290 
   Size:       3857
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    678    2528    2712     678 
   Size:       1850     184    1823

  Fragments arranged by size: 

                1850    1823     184
 
MscI TGG'CCA

Cuts at:   1618    1618 
   Size:       3857
 
MslI CAynn'nnrTG

Cuts at:    664    1846    1973     664 
   Size:       1182     127    2548

  Fragments arranged by size: 

                2548    1182     127
 
MspA1I CmG'CkG

Cuts at:    968    1282    1642    2655    2900     968 
   Size:        314     360    1013     245    1925

  Fragments arranged by size: 

                1925    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2038    2038 
   Size:       3857
 
NarI GG'CG_CC

Cuts at:   1288    1536    1288 
   Size:        248    3609
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3857
 
NgoMIV G'CCGG_C

Cuts at:   2036    2036 
   Size:       3857
 
NspI r_CATG'y

Cuts at:    504     910    1410    2317     504 
   Size:        406     500     907    2044

  Fragments arranged by size: 

                2044     907     500     406
 
PciI A'CATG_T

Cuts at:   2313    2313 
   Size:       3857
 
PflMI CCAn_nnn'nTGG

Cuts at:    760     760 
   Size:       3857
 
PmeI GTTT'AAAC

Cuts at:    856     856 
   Size:       3857
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2996    3028    2996 
   Size:         32    3825
 
PstI C_TGCA'G

Cuts at:    363     904    1589     363 
   Size:        541     685    2631

  Fragments arranged by size: 

                2631     685     541
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3857
 
PvuII CAG'CTG

Cuts at:   1282    1642    1282 
   Size:        360    3497
 
RsrII CG'GwC_CG

Cuts at:   2052    2052 
   Size:       3857
 
SacI G_AGCT'C

Cuts at:    871     871 
   Size:       3857
 
SalI G'TCGA_C

Cuts at:    894     894 
   Size:       3857
 
SapI GCTCTTCn'nnn_

Cuts at:   1880    2090    1880 
   Size:        210    3647
 
SbfI CC_TGCA'GG

Cuts at:    904     904 
   Size:       3857
 
ScaI AGT'ACT

Cuts at:   3374    3374 
   Size:       3857
 
SfcI C'TryA_G

Cuts at:     33     359     900    1585    2578    2769      33 
   Size:        326     541     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     541     326     191
 
SfoI GGC'GCC

Cuts at:   1289    1537    1289 
   Size:        248    3609
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3857
 
SmaI CCC'GGG

Cuts at:    879     879 
   Size:       3857
 
SmlI C'TyrA_G

Cuts at:    160    1012    1927    2419    2681     160 
   Size:        852     915     492     262    1336

  Fragments arranged by size: 

                1336     915     852     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3857
 
SphI G_CATG'C

Cuts at:    910    1410     910 
   Size:        500    3357
 
StyI C'CwwG_G

Cuts at:    107     991    1345     107 
   Size:        884     354    2619

  Fragments arranged by size: 

                2619     884     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    796    1440    1573    2032    2241     796 
   Size:        644     133     459     209    2412

  Fragments arranged by size: 

                2412     644     459     209     133
 
TatI w'GTAC_w

Cuts at:    299    3372     299 
   Size:       3073     784
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    458     490    3374    3406     458 
   Size:         32    2884      32     909

  Fragments arranged by size: 

                2884     909      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1654    1654 
   Size:       3857
 
XbaI T'CTAG_A

Cuts at:    888     888 
   Size:       3857
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    527    1352     527 
   Size:        825    3032
 
XmaI C'CCGG_G

Cuts at:    877     877 
   Size:       3857
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BglII     BlpI
 Bme1580I     BmrI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
    BstYI     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PciI    PflMI     PmeI     PpiI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI    BfrBI     BglI    BmgBI     BmtI     BpmI
    BsaXI    BseRI     BsgI    BsiWI     BsmI    BsmBI   BssHII   BstEII
    BstXI  BstZ17I   Bsu36I     ClaI    EcoNI    EcoRV     FseI    FspAI
     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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