Restriction map for pF1KB3331
 (Linear) (Six-base) MAPSORT of: KIBB3331.seq  Check: 8598  from: 1  to: 1542



KIBB3331.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AccI GT'mk_AC

Cuts at:      0    1463    1542 
   Size:       1463      79
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1460    1542 
   Size:       1460      82
 
AflIII A'CryG_T

Cuts at:      0      68    1542 
   Size:         68    1474
 
AleI CACnn'nnGTG

Cuts at:      0      37    1542 
   Size:         37    1505
 
AlwNI CAG_nnn'CTG

Cuts at:      0     310    1542 
   Size:        310    1232
 
ApoI r'AATT_y

Cuts at:      0     486    1195    1263    1542 
   Size:        486     709      68     279

  Fragments arranged by size: 

                 709     486     279      68
 
BanI G'GyrC_C

Cuts at:      0     569    1300    1542 
   Size:        569     731     242

  Fragments arranged by size: 

                 731     569     242
 
BanII G_rGCy'C

Cuts at:      0     775    1542 
   Size:        775     767
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     803    1542 
   Size:        803     739
 
BclI T'GATC_A

Cuts at:      0    1410    1542 
   Size:       1410     132
 
BfrBI ATG'CAT

Cuts at:      0    1308    1542 
   Size:       1308     234
 
BglII A'GATC_T

Cuts at:      0     686    1542 
   Size:        686     856
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     558     737    1542 
   Size:        558     179     805

  Fragments arranged by size: 

                 805     558     179
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1157    1307    1341    1542 
   Size:       1157     150      34     201

  Fragments arranged by size: 

                1157     201     150      34
 
Bpu10I CC'TnA_GC

Cuts at:      0     375    1542 
   Size:        375    1167
 
BsaBI GATnn'nnATC

Cuts at:      0     413    1542 
   Size:        413    1129
 
BsaWI w'CCGG_w

Cuts at:      0    1228    1542 
   Size:       1228     314
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1265    1295    1542 
   Size:       1265      30     247

  Fragments arranged by size: 

                1265     247      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      22    1542 
   Size:         22    1520
 
BseYI C'CCAG_C

Cuts at:      0     614     815    1542 
   Size:        614     201     727

  Fragments arranged by size: 

                 727     614     201
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     350     509     887     917    1542 
   Size:        350     159     378      30     625

  Fragments arranged by size: 

                 625     378     350     159      30
 
BsmI GAATG_Cn'

Cuts at:      0     219     353    1542 
   Size:        219     134    1189

  Fragments arranged by size: 

                1189     219     134
 
Bsp1286I G_dGCh'C

Cuts at:      0     775    1542 
   Size:        775     767
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     274    1542 
   Size:        274    1268
 
BsrGI T'GTAC_A

Cuts at:      0      27     966    1542 
   Size:         27     939     576

  Fragments arranged by size: 

                 939     576      27
 
BstEII G'GTnAC_C

Cuts at:      0     909    1542 
   Size:        909     633
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1508    1542 
   Size:       1508      34
 
BstYI r'GATC_y

Cuts at:      0     338     664     686     819    1071    1542 
   Size:        338     326      22     133     252     471

  Fragments arranged by size: 

                 471     338     326     252     133      22
 
BstZ17I GTA'TAC

Cuts at:      0    1464    1542 
   Size:       1464      78
 
BtgI C'CryG_G

Cuts at:      0     577    1542 
   Size:        577     965
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     496    1542 
   Size:        496    1046
 
BtsI GCAGTG_nn'

Cuts at:      0     875    1195    1542 
   Size:        875     320     347

  Fragments arranged by size: 

                 875     347     320
 
ClaI AT'CG_AT

Cuts at:      0     899    1542 
   Size:        899     643
 
EarI CTCTTCn'nnn_

Cuts at:      0      49     104    1542 
   Size:         49      55    1438

  Fragments arranged by size: 

                1438      55      49
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1157    1307    1341    1460    1542 
   Size:       1157     150      34     119      82

  Fragments arranged by size: 

                1157     150     119      82      34
 
EcoO109I rG'GnC_Cy

Cuts at:      0      59     205     307    1542 
   Size:         59     146     102    1235

  Fragments arranged by size: 

                1235     146     102      59
 
EcoRI G'AATT_C

Cuts at:      0     486    1542 
   Size:        486    1056
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     208    1542 
   Size:        208    1334
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     762     794    1369    1401    1542 
   Size:        762      32     575      32     141

  Fragments arranged by size: 

                 762     575     141      32      32
 
HindIII A'AGCT_T

Cuts at:      0     139    1542 
   Size:        139    1403
 
MfeI C'AATT_G

Cuts at:      0     491    1542 
   Size:        491    1051
 
MslI CAynn'nnrTG

Cuts at:      0      37     851     863     989    1542 
   Size:         37     814      12     126     553

  Fragments arranged by size: 

                 814     553     126      37      12
 
MspA1I CmG'CkG

Cuts at:      0     364    1357    1438    1508    1542 
   Size:        364     993      81      70      34

  Fragments arranged by size: 

                 993     364      81      70      34
 
NdeI CA'TA_TG

Cuts at:      0     170     228    1542 
   Size:        170      58    1314

  Fragments arranged by size: 

                1314     170      58
 
NsiI A_TGCA'T

Cuts at:      0    1310    1542 
   Size:       1310     232
 
NspI r_CATG'y

Cuts at:      0      72    1542 
   Size:         72    1470
 
PciI A'CATG_T

Cuts at:      0      68    1542 
   Size:         68    1474
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     310    1542 
   Size:        310    1232
 
PpuMI rG'GwC_Cy

Cuts at:      0      59     205     307    1542 
   Size:         59     146     102    1235

  Fragments arranged by size: 

                1235     146     102      59
 
PstI C_TGCA'G

Cuts at:      0    1023    1542 
   Size:       1023     519
 
PvuII CAG'CTG

Cuts at:      0     364    1357    1438    1508    1542 
   Size:        364     993      81      70      34

  Fragments arranged by size: 

                 993     364      81      70      34
 
ScaI AGT'ACT

Cuts at:      0     741    1542 
   Size:        741     801
 
SfcI C'TryA_G

Cuts at:      0     476     588     915    1001    1019    1542 
   Size:        476     112     327      86      18     523

  Fragments arranged by size: 

                 523     476     327     112      86      18
 
SspI AAT'ATT

Cuts at:      0    1394    1542 
   Size:       1394     148
 
StyI C'CwwG_G

Cuts at:      0     401    1542 
   Size:        401    1141
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     587    1542 
   Size:        587     955
 
TatI w'GTAC_w

Cuts at:      0      27     739     966    1542 
   Size:         27     712     227     576

  Fragments arranged by size: 

                 712     576     227      27
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      80     112    1542 
   Size:         80      32    1430

  Fragments arranged by size: 

                1430      80      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     563    1542 
   Size:        563     979
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AleI    AlwNI     ApoI     BanI    BanII
     BbsI     BclI    BfrBI    BglII     BmrI     BpmI   Bpu10I    BsaBI
    BsaWI    BsaXI    BseRI    BseYI     BsgI     BsmI Bsp1286I    BspMI
    BsrGI   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI     BtsI
     ClaI     EarI  Eco57MI EcoO109I    EcoRI     FalI    Hin4I  HindIII
     MfeI     MslI   MspA1I     NdeI     NsiI     NspI     PciI    PflMI
    PpuMI     PstI    PvuII     ScaI     SfcI     SspI     StyI    TaqII
     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BglI
     BlpI Bme1580I    BmgBI     BmtI     BplI    BpuEI     BsaI    BsaAI
    BsaHI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   Bsu36I    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
    EcoRV     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI
     KpnI     MluI     MmeI     MscI     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     PacI     PasI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI
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