Restriction map for
pF1KE0985
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII AccI AgeI AhdI Alw44I
AlwNI ApaLI AscI AseI AsnI
AspEI AviII AvrII BbeI BbrPI
BbsI BcgI BciVI BlnI BpuAI
BsaAI BsaBI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoNI
EcoO65I EcoRV EcoT22I FseI FspI
HindIII HpaI KasI MamI MfeI
MluI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI PvuI RsrII SalI
SbfI ScaI SexAI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 190 GGTACC G^GTACC
AccII 258 CGCG CG^CG
AccIII 132 TCCGGA T^CCGGA
AclI 1477 AACGTT AA^CGTT
AcyI 1655 GACGCC G[GA]^CG[CT]C
AflIII 1391 ACATGT A^C[GA][CT]GT
Aor51HI 1729 AGCGCT AGC^GCT
Asp700I 1710 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 190 GGTACC G^GTACC
AspI 1543 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 819 GCTGAGG CCTCAGC(-5/-2)
BclI 1409 TGATCA T^GATCA
BfaI 212 CTAG C^TAG
BglI 638 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1655 GACGCC G[GA]^CG[CT]C
BsaWI 132 TCCGGA [AT]^CCGG[AT]
BseAI 132 TCCGGA T^CCGGA
BseRI 748 GAGGAG GAGGAG(10/8)
BspEI 132 TCCGGA T^CCGGA
BspLU11I 1391 ACATGT A^CATGT
BsrBI 1428 GAGCGG CCGCTC(-3/-3)
BsrDI 1719 CATTGC GCAATG(2/0)
BstUI 258 CGCG CG^CG
BstXI 360 CCAGGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eco47III 1729 AGCGCT AGC^GCT
FbaI 1409 TGATCA T^GATCA
Hin1I 1655 GACGCC G[GA]^CG[CT]C
HincII 1243 GTCAAC GT[CT]^[GA]AC
HindII 1243 GTCAAC GT[CT]^[GA]AC
KpnI 190 GGTACC GGTAC^C
KspI 257 CCGCGG CCGC^GG
MaeI 212 CTAG C^TAG
MaeII 1478 ACGT A^CGT
MroI 132 TCCGGA T^CCGGA
MvnI 258 CGCG CG^CG
NcoI 1148 CCATGG C^CATGG
PaeR7I 672 CTCGAG C^TCGAG
PflFI 1543 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 762 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 315 GACCAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1477 AACGTT AA^CGTT
PstI 92 CTGCAG CTGCA^G
SacII 257 CCGCGG CCGC^GG
SapI 1098 GCTCTTC GCTCTTC(1/4)
SfcI 92 CTGCAG C^T[GA][CT]AG
SfiI 637 GGCCAGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 921 AGGCCT AGG^CCT
Tth111I 1543 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 762 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1250 CCACCGGGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 672 CTCGAG C^TCGAG
XmnI 1710 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 32 GAATTC [GA]^AATT[CT]
844 GAATTC
AflII 1660 CTTAAG C^TTAAG
1678 CTTAAG
AocI 350 CCTCAGG CC^T[ACGT]AGG
1351 CCTGAGG
ApoI 32 GAATTC [GA]^AATT[CT]
844 GAATTC
AspHI 517 GAGCTC G[AT]GC[AT]^C
1096 GAGCTC
AvaI 672 CTCGAG C^[CT]CG[GA]G
1761 CTCGGG
BalI 488 TGGCCA TGG^CCA
645 TGGCCA
BfrI 1660 CTTAAG C^TTAAG
1678 CTTAAG
BglII 1166 AGATCT A^GATCT
1670 AGATCT
BlpI 337 GCTGAGC GC^T[ACGT]AGC
1425 GCTGAGC
BpmI 468 CTCCAG CTGGAG(16/14)
985 CTGGAG
Bpu1102I 337 GCTGAGC GC^T[ACGT]AGC
1425 GCTGAGC
BsiHKAI 517 GAGCTC G[AT]GC[AT]^C
1096 GAGCTC
BsoBI 672 CTCGAG C^[CT]CG[GA]G
1761 CTCGGG
BspHI 998 TCATGA T^CATGA
1691 TCATGA
BspMI 429 ACCTGC ACCTGC(4/8)
901 ACCTGC
Bsu36I 350 CCTCAGG CC^T[ACGT]AGG
1351 CCTGAGG
CelII 337 GCTGAGC GC^T[ACGT]AGC
1425 GCTGAGC
EaeI 488 TGGCCA [CT]^GGCC[GA]
645 TGGCCA
Eco81I 350 CCTCAGG CC^T[ACGT]AGG
1351 CCTGAGG
EcoRI 32 GAATTC G^AATTC
844 GAATTC
HgaI 1655 GACGC GACGC(5/10)
320 GCGTC
HinfI 957 GACTC G^A[ACGT]TC
1050 GACTC
MluNI 488 TGGCCA TGG^CCA
645 TGGCCA
MscI 488 TGGCCA TGG^CCA
645 TGGCCA
MseI 1661 TTAA T^TAA
1679 TTAA
MslI 1146 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1413 CACCTACATG
NspI 1391 ACATGT [GA]CATG^[CT]
1418 ACATGC
PleI 957 GACTC GAGTC(4/5)
1050 GACTC
PpuMI 66 AGGTCCC [GA]G^G[AT]CC[CT]
1330 GGGACCC
RcaI 998 TCATGA T^CATGA
1691 TCATGA
SacI 517 GAGCTC GAGCT^C
1096 GAGCTC
Tru9I 1661 TTAA T^TAA
1679 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AfaI AluI AlwI ApaI
AvaII BamHI BanI BanII BbvI
BcnI BmyI BsaI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII