Restriction map for pF1KE0968
 (Circular) (Six-base) MAPSORT of: pF1KE0968.seq  Check: 9155  from: 1  to: 4673



pF1KE0968  4673 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AccI GT'mk_AC

Cuts at:    890    1556    1711     890 
   Size:        666     155    3852

  Fragments arranged by size: 

                3852     666     155
 
Acc65I G'GTAC_C

Cuts at:   1689    1689 
   Size:       4673
 
AclI AA'CG_TT

Cuts at:   1649    4513    1649 
   Size:       2864    1809
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    116     406     574     696    2498    2930    3677    3945     116 
   Size:        290     168     122    1802     432     747     268     844

  Fragments arranged by size: 

                1802     844     747     432     290     268     168     122
            
 
AflIII A'CryG_T

Cuts at:    893     965    2025    3129    4106     893 
   Size:         72    1060    1104     977    1460

  Fragments arranged by size: 

                1460    1104    1060     977      72
 
AgeI A'CCGG_T

Cuts at:   3061    3061 
   Size:       4673
 
AleI CACnn'nnGTG

Cuts at:   1198    1198 
   Size:       4673
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    283     315     283 
   Size:         32    4641
 
AlwNI CAG_nnn'CTG

Cuts at:    543    1350    1978    3545     543 
   Size:        807     628    1567    1671

  Fragments arranged by size: 

                1671    1567     807     628
 
ApaLI G'TGCA_C

Cuts at:   3443    3443 
   Size:       4673
 
ApoI r'AATT_y

Cuts at:    343    1677    4026    4282     343 
   Size:       1334    2349     256     734

  Fragments arranged by size: 

                2349    1334     734     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4673
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4673
 
AvaI C'yCGr_G

Cuts at:   1021    1416    1693    1021 
   Size:        395     277    4001

  Fragments arranged by size: 

                4001     395     277
 
BamHI G'GATC_C

Cuts at:    168    1698     168 
   Size:       1530    3143
 
BanI G'GyrC_C

Cuts at:   1689    2103    2351    2386    1689 
   Size:        414     248      35    3976

  Fragments arranged by size: 

                3976     414     248      35
 
BanII G_rGCy'C

Cuts at:    125     662    1687    2717     125 
   Size:        537    1025    1030    2081

  Fragments arranged by size: 

                2081    1030    1025     537
 
BbeI G_GCGC'C

Cuts at:   2107    2355    2107 
   Size:        248    4425
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    4639
 
BciVI GTATCCnnnnn_n'

Cuts at:   2571    3332    4673    2571 
   Size:        761    1341    2571

  Fragments arranged by size: 

                2571    1341     761
 
BclI T'GATC_A

Cuts at:    399    1500    2193     399 
   Size:       1101     693    2879

  Fragments arranged by size: 

                2879    1101     693
 
BglI GCCn_nnn'nGGC

Cuts at:    112     112 
   Size:       4673
 
BglII A'GATC_T

Cuts at:   2188    4225    2188 
   Size:       2037    2636
 
BlpI GC'TnA_GC

Cuts at:   1785    1785 
   Size:       4673
 
Bme1580I G_kGCm'C

Cuts at:   2298    2391    3447    2298 
   Size:         93    1056    3524

  Fragments arranged by size: 

                3524    1056      93
 
BmgBI CAC'GTC

Cuts at:    954     954 
   Size:       4673
 
BmrI ACTGGGnnnn_n'

Cuts at:   1885    1966    2301    4445    1885 
   Size:         81     335    2144    2113

  Fragments arranged by size: 

                2144    2113     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3040    3040 
   Size:       4673
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    448    1086    1501     448 
   Size:        638     415    3620

  Fragments arranged by size: 

                3620     638     415
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    109     784    1276    1309    1621    1849    2728    3220    3518 
   Size:        675     492      33     312     228     879     492     298

Cuts at:   3518     109 
   Size:       1264

  Fragments arranged by size: 

                1264     879     675     492     492     312     298     228
                  33
 
BsaI GGTCTCn'nnnn_

Cuts at:   4377    4377 
   Size:       4673
 
BsaAI yAC'GTr

Cuts at:    896    2026     896 
   Size:       1130    3543
 
BsaBI GATnn'nnATC

Cuts at:      6    2187    4224       6 
   Size:       2181    2037     455

  Fragments arranged by size: 

                2181    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   2104    2352    3054    4550    2104 
   Size:        248     702    1496    2227

  Fragments arranged by size: 

                2227    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1862    2383    3061    3335    3482    3893    1862 
   Size:        521     678     274     147     411    2642

  Fragments arranged by size: 

                2642     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    283     313     283 
   Size:         30    4643
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    196     196 
   Size:       4673
 
BseYI C'CCAG_C

Cuts at:   1065    2098    2856    3433    3929    1065 
   Size:       1033     758     577     496    1809

  Fragments arranged by size: 

                1809    1033     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    2215    2261    3469     104 
   Size:       2111      46    1208    1308

  Fragments arranged by size: 

                2111    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    125     662    1687    2465    2655    3447     125 
   Size:        537    1025     778     190     792    1351

  Fragments arranged by size: 

                1351    1025     792     778     537     190
 
BsmI GAATG_Cn'

Cuts at:   1491    1491 
   Size:       4673
 
Bsp1286I G_dGCh'C

Cuts at:    125     662    1687    2298    2391    2465    2655    2717    3447 
   Size:        537    1025     611      93      74     190      62     730

Cuts at:   3447     125 
   Size:       1351

  Fragments arranged by size: 

                1351    1025     730     611     537     190      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:   3893    3893 
   Size:       4673
 
BspHI T'CATG_A

Cuts at:   3849    3849 
   Size:       4673
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1723    1937    2239    2620    1723 
   Size:        214     302     381    3776

  Fragments arranged by size: 

                3776     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2965    3019    4503    4668      50 
   Size:       2915      54    1484     165      55

  Fragments arranged by size: 

                2915    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    404    2585     404 
   Size:       2181    2492
 
BsrFI r'CCGG_y

Cuts at:    111     908    1081    2671    2852    3061     111 
   Size:        797     173    1590     181     209    1723

  Fragments arranged by size: 

                1723    1590     797     209     181     173
 
BsrGI T'GTAC_A

Cuts at:    998     998 
   Size:       4673
 
BssSI C'ACGA_G

Cuts at:   2944    3302    2944 
   Size:        358    4315
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2050    2050 
   Size:       4673
 
BstBI TT'CG_AA

Cuts at:   3034    3034 
   Size:       4673
 
BstXI CCAn_nnnn'nTGG

Cuts at:    471    1227     471 
   Size:        756    3917
 
BstYI r'GATC_y

Cuts at:      1     168     557    1698    2165    2188    2523    2769    3781 
   Size:        167     389    1141     467      23     335     246    1012

Cuts at:   3781    3867    3879    4225       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1141    1012     467     449     389     346     335     246
                 167      86      23      12
 
BstZ17I GTA'TAC

Cuts at:    891     891 
   Size:       4673
 
Bsu36I CC'TnA_GG

Cuts at:   1351    1351 
   Size:       4673
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2623    2804    2967    2623 
   Size:        181     163    4329

  Fragments arranged by size: 

                4329     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1916    1916 
   Size:       4673
 
DraI TTT'AAA

Cuts at:   1672    1672 
   Size:       4673
 
DrdI GACnn_nn'nnGTC

Cuts at:   2379    3237    2379 
   Size:        858    3815
 
EaeI y'GGCC_r

Cuts at:    461     956    2258    2432    2823    2850    4195     461 
   Size:        495    1302     174     391      27    1345     939

  Fragments arranged by size: 

                1345    1302     939     495     391     174      27
 
EagI C'GGCC_G

Cuts at:   2258    2258 
   Size:       4673
 
EarI CTCTTCn'nnn_

Cuts at:    517    1271    2696    2906     517 
   Size:        754    1425     210    2284

  Fragments arranged by size: 

                2284    1425     754     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1006    1606    2881    3191    3337    4483    1006 
   Size:        600    1275     310     146    1146    1196

  Fragments arranged by size: 

                1275    1196    1146     600     310     146
 
EcoICRI GAG'CTC

Cuts at:    123     660    1685     123 
   Size:        537    1025    3111

  Fragments arranged by size: 

                3111    1025     537
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    116     406     448     574     696    1086    1501    2498    2930 
   Size:        290      42     126     122     390     415     997     432

Cuts at:   2930    3677    3945     116 
   Size:        747     268     844

  Fragments arranged by size: 

                 997     844     747     432     415     390     290     268
                 126     122      42
 
EcoO109I rG'GnC_Cy

Cuts at:    228     390     446     540    1188    1287    1340    1812     228 
   Size:        162      56      94     648      99      53     472    3089

  Fragments arranged by size: 

                3089     648     472     162      99      94      56      53
            
 
EcoRI G'AATT_C

Cuts at:   1677    1677 
   Size:       4673
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    465    2692     465 
   Size:       2227    2446
 
FspI TGC'GCA

Cuts at:   2454    2454 
   Size:       4673
 
HaeII r_GCGC'y

Cuts at:    735    2107    2355    3377    4356     735 
   Size:       1372     248    1022     979    1052

  Fragments arranged by size: 

                1372    1052    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    172     204     847     879    2515    2545    2547    2577     172 
   Size:         32     643      32    1636      30       2      30    2268

  Fragments arranged by size: 

                2268    1636     643      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1712    1712 
   Size:       4673
 
HindIII A'AGCT_T

Cuts at:    235     235 
   Size:       4673
 
KasI G'GCGC_C

Cuts at:   2103    2351    2103 
   Size:        248    4425
 
KpnI G_GTAC'C

Cuts at:   1693    1693 
   Size:       4673
 
MluI A'CGCG_T

Cuts at:   4106    4106 
   Size:       4673
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3344    3528    3344 
   Size:        184    4489
 
MscI TGG'CCA

Cuts at:    463     958    2434     463 
   Size:        495    1476    2702

  Fragments arranged by size: 

                2702    1476     495
 
MslI CAynn'nnrTG

Cuts at:    964    1060    1198    1496    2662    2789     964 
   Size:         96     138     298    1166     127    2848

  Fragments arranged by size: 

                2848    1166     298     138     127      96
 
MspA1I CmG'CkG

Cuts at:    220     252    1044    1542    1582    1784    2098    2458    3471 
   Size:         32     792     498      40     202     314     360    1013

Cuts at:   3471    3716     220 
   Size:        245    1177

  Fragments arranged by size: 

                1177    1013     792     498     360     314     245     202
                  40      32
 
NaeI GCC'GGC

Cuts at:    113    1083    2854     113 
   Size:        970    1771    1932

  Fragments arranged by size: 

                1932    1771     970
 
NarI GG'CG_CC

Cuts at:   2104    2352    2104 
   Size:        248    4425
 
NdeI CA'TA_TG

Cuts at:    261     611     261 
   Size:        350    4323
 
NgoMIV G'CCGG_C

Cuts at:    111    1081    2852     111 
   Size:        970    1771    1932

  Fragments arranged by size: 

                1932    1771     970
 
NspI r_CATG'y

Cuts at:    969    1300    1486    1726    2226    3133     969 
   Size:        331     186     240     500     907    2509

  Fragments arranged by size: 

                2509     907     500     331     240     186
 
PciI A'CATG_T

Cuts at:    965    3129     965 
   Size:       2164    2509
 
PfoI T'CCnGG_A

Cuts at:    560    1102    1226     560 
   Size:        542     124    4007

  Fragments arranged by size: 

                4007     542     124
 
PmeI GTTT'AAAC

Cuts at:   1672    1672 
   Size:       4673
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    283     315    3812    3844     283 
   Size:         32    3497      32    1112

  Fragments arranged by size: 

                3497    1112      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    390     446    1188     390 
   Size:         56     742    3875

  Fragments arranged by size: 

                3875     742      56
 
PspXI vC'TCGA_Gb

Cuts at:   1416    1416 
   Size:       4673
 
PstI C_TGCA'G

Cuts at:   1720    2405    1720 
   Size:        685    3988
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4673
 
PvuII CAG'CTG

Cuts at:    252    1044    1542    1582    2098    2458     252 
   Size:        792     498      40     516     360    2467

  Fragments arranged by size: 

                2467     792     516     498     360      40
 
RsrII CG'GwC_CG

Cuts at:   2868    2868 
   Size:       4673
 
SacI G_AGCT'C

Cuts at:    125     662    1687     125 
   Size:        537    1025    3111

  Fragments arranged by size: 

                3111    1025     537
 
SalI G'TCGA_C

Cuts at:   1710    1710 
   Size:       4673
 
SapI GCTCTTCn'nnn_

Cuts at:   2696    2906    2696 
   Size:        210    4463
 
SbfI CC_TGCA'GG

Cuts at:   1720    1720 
   Size:       4673
 
ScaI AGT'ACT

Cuts at:   4190    4190 
   Size:       4673
 
SfcI C'TryA_G

Cuts at:     33    1451    1716    2401    3394    3585      33 
   Size:       1418     265     685     993     191    1121

  Fragments arranged by size: 

                1418    1121     993     685     265     191
 
SfoI GGC'GCC

Cuts at:   2105    2353    2105 
   Size:        248    4425
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4673
 
SmaI CCC'GGG

Cuts at:   1023    1695    1023 
   Size:        672    4001
 
SmlI C'TyrA_G

Cuts at:    124     799    1291    1324    1416    1636    1828    2743    3235 
   Size:        675     492      33      92     220     192     915     492

Cuts at:   3235    3497     124 
   Size:        262    1300

  Fragments arranged by size: 

                1300     915     675     492     492     262     220     192
                  92      33
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4673
 
SphI G_CATG'C

Cuts at:   1300    1726    2226    1300 
   Size:        426     500    3747

  Fragments arranged by size: 

                3747     500     426
 
StyI C'CwwG_G

Cuts at:    173    1184    1807    2161     173 
   Size:       1011     623     354    2685

  Fragments arranged by size: 

                2685    1011     623     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2256    2389    2848    3057    2256 
   Size:        133     459     209    3872

  Fragments arranged by size: 

                3872     459     209     133
 
TatI w'GTAC_w

Cuts at:    998    4188     998 
   Size:       3190    1483
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4190    4222    4190 
   Size:         32    4641
 
Tth111I GACn'n_nGTC

Cuts at:   2470    2470 
   Size:       4673
 
XbaI T'CTAG_A

Cuts at:   1704    1704 
   Size:       4673
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2168    2168 
   Size:       4673
 
XhoI C'TCGA_G

Cuts at:   1416    1416 
   Size:       4673
 
XmaI C'CCGG_G

Cuts at:   1021    1693    1021 
   Size:        672    4001
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI     AloI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NspI     PciI     PfoI
     PmeI     PpiI    PpuMI    PspXI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AlfI     ApaI     AscI
    AvrII     BaeI     BbsI    BbvCI    BfrBI     BmtI   Bpu10I     BsgI
    BsiWI    BsmBI   BssHII   BstEII     BtgI     ClaI    CspCI   DraIII
    EcoNI    EcoRV     FseI    FspAI     HpaI     MfeI     NcoI     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PmlI    PshAI
     PsiI   PspOMI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI
    SnaBI     SrfI     SspI     StuI     SwaI     XmnI     ZraI
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