Restriction map for pF1KE0967
 (Linear) (Six-base) MAPSORT of: KIEE0967.seq  Check: 8796  from: 1  to: 1539



KIEE0967.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AccI GT'mk_AC

Cuts at:      0     704    1539 
   Size:        704     835
 
Acc65I G'GTAC_C

Cuts at:      0    1081    1539 
   Size:       1081     458
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      35     314    1539 
   Size:         35     279    1225

  Fragments arranged by size: 

                1225     279      35
 
AflII C'TTAA_G

Cuts at:      0     835    1539 
   Size:        835     704
 
AflIII A'CryG_T

Cuts at:      0    1232    1539 
   Size:       1232     307
 
AhdI GACnn_n'nnGTC

Cuts at:      0     678    1539 
   Size:        678     861
 
AleI CACnn'nnGTG

Cuts at:      0    1498    1539 
   Size:       1498      41
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     197    1358    1539 
   Size:        197    1161     181

  Fragments arranged by size: 

                1161     197     181
 
ApoI r'AATT_y

Cuts at:      0    1036    1539 
   Size:       1036     503
 
BanI G'GyrC_C

Cuts at:      0     432     766    1081    1539 
   Size:        432     334     315     458

  Fragments arranged by size: 

                 458     432     334     315
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1283    1539 
   Size:       1283     256
 
BclI T'GATC_A

Cuts at:      0     794    1539 
   Size:        794     745
 
BfrBI ATG'CAT

Cuts at:      0    1141    1539 
   Size:       1141     398
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     621    1539 
   Size:        621     918
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1085    1539 
   Size:       1085     454
 
Bpu10I CC'TnA_GC

Cuts at:      0     395    1539 
   Size:        395    1144
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     509    1539 
   Size:        509    1030
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     855    1539 
   Size:        855     684
 
BsaBI GATnn'nnATC

Cuts at:      0     782    1539 
   Size:        782     757
 
BsaWI w'CCGG_w

Cuts at:      0     351    1539 
   Size:        351    1188
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1261    1539 
   Size:       1261     278
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     773    1539 
   Size:        773     766
 
BspEI T'CCGG_A

Cuts at:      0     351    1539 
   Size:        351    1188
 
BspHI T'CATG_A

Cuts at:      0     807    1539 
   Size:        807     732
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     954    1539 
   Size:        954     585
 
BsrDI GCAATG_nn'

Cuts at:      0    1010    1361    1539 
   Size:       1010     351     178

  Fragments arranged by size: 

                1010     351     178
 
BsrGI T'GTAC_A

Cuts at:      0     479    1539 
   Size:        479    1060
 
BstYI r'GATC_y

Cuts at:      0     783    1539 
   Size:        783     756
 
BtgI C'CryG_G

Cuts at:      0     884    1068    1166    1539 
   Size:        884     184      98     373

  Fragments arranged by size: 

                 884     373     184      98
 
BtsI GCAGTG_nn'

Cuts at:      0     571    1539 
   Size:        571     968
 
EaeI y'GGCC_r

Cuts at:      0     936    1257    1539 
   Size:        936     321     282

  Fragments arranged by size: 

                 936     321     282
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      35     314    1085    1539 
   Size:         35     279     771     454

  Fragments arranged by size: 

                 771     454     279      35
 
EcoRV GAT'ATC

Cuts at:      0     246    1539 
   Size:        246    1293
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     664     696    1539 
   Size:        664      32     843

  Fragments arranged by size: 

                 843     664      32
 
HincII GTy'rAC

Cuts at:      0    1512    1539 
   Size:       1512      27
 
KpnI G_GTAC'C

Cuts at:      0    1085    1539 
   Size:       1085     454
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     544    1539 
   Size:        544     995
 
MscI TGG'CCA

Cuts at:      0     938    1259    1539 
   Size:        938     321     280

  Fragments arranged by size: 

                 938     321     280
 
MslI CAynn'nnrTG

Cuts at:      0    1498    1539 
   Size:       1498      41
 
MspA1I CmG'CkG

Cuts at:      0    1414    1539 
   Size:       1414     125
 
NcoI C'CATG_G

Cuts at:      0     884    1068    1166    1539 
   Size:        884     184      98     373

  Fragments arranged by size: 

                 884     373     184      98
 
NsiI A_TGCA'T

Cuts at:      0    1143    1539 
   Size:       1143     396
 
NspI r_CATG'y

Cuts at:      0    1236    1284    1345    1539 
   Size:       1236      48      61     194

  Fragments arranged by size: 

                1236     194      61      48
 
PciI A'CATG_T

Cuts at:      0    1232    1539 
   Size:       1232     307
 
PstI C_TGCA'G

Cuts at:      0     578    1059    1212    1539 
   Size:        578     481     153     327

  Fragments arranged by size: 

                 578     481     327     153
 
PvuII CAG'CTG

Cuts at:      0    1414    1539 
   Size:       1414     125
 
SfcI C'TryA_G

Cuts at:      0     574     705    1055    1208    1220    1539 
   Size:        574     131     350     153      12     319

  Fragments arranged by size: 

                 574     350     319     153     131      12
 
SmlI C'TyrA_G

Cuts at:      0     524     835    1539 
   Size:        524     311     704

  Fragments arranged by size: 

                 704     524     311
 
SspI AAT'ATT

Cuts at:      0     198     659    1461    1539 
   Size:        198     461     802      78

  Fragments arranged by size: 

                 802     461     198      78
 
StuI AGG'CCT

Cuts at:      0    1314    1539 
   Size:       1314     225
 
StyI C'CwwG_G

Cuts at:      0     428     884    1068    1166    1539 
   Size:        428     456     184      98     373

  Fragments arranged by size: 

                 456     428     373     184      98
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     465    1539 
   Size:        465    1074
 
TatI w'GTAC_w

Cuts at:      0     479     510    1201    1539 
   Size:        479      31     691     338

  Fragments arranged by size: 

                 691     479     338      31
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     255     287     366     398    1539 
   Size:        255      32      79      32    1141

  Fragments arranged by size: 

                1141     255      79      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     777    1075    1539 
   Size:        777     298     464

  Fragments arranged by size: 

                 777     464     298
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI    AflII   AflIII     AhdI     AleI     AlfI
     ApoI     BanI     BbsI     BclI    BfrBI     BmrI     BpmI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaWI    BseRI     BsgI    BspEI    BspHI
    BspMI    BsrDI    BsrGI    BstYI     BtgI     BtsI     EaeI  Eco57MI
    EcoRV    Hin4I   HincII     KpnI     MmeI     MscI     MslI   MspA1I
     NcoI     NsiI     NspI     PciI     PstI    PvuII     SfcI     SmlI
     SspI     StuI     StyI    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI     AgeI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BanII     BbeI    BbvCI     BcgI    BciVI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI    BsaAI    BsaHI    BsaXI    BseYI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BsrBI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     MfeI     MluI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SwaI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI
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