Restriction map for pF1KE0959
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        AlwI        Aor51HI     ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       BamHI       BbeI        BbrPI       
BcgI        BciVI       BclI        BfrI        BglII       
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrBI       BsrDI       BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     ClaI        CpoI        DpnII       
DraI        DraIII      DrdI        Eam1105I    Eco47III    
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HaeII       HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        MaeII       MamI        MboI        MfeI        
MflI        MluI        MroI        MseI        MunI        
NarI        NdeI        NdeII       NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacI        SalI        SapI        Sau3AI      
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SspBI       SspI        SwaI        TaqI        Tru9I       
Tsp509I     Tth111I     TthHB8I     XbaI        XhoI        
XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   26 enzymes) #
###################################

AccII        1085  CGCG                 CG^CG
AvrII        1305  CCTAGG               C^CTAGG
BalI           23  TGGCCA               TGG^CCA
BbvCI        1464  CCTCAGC              CCTCAGC(-5/-2)
BlnI         1305  CCTAGG               C^CTAGG
BsaI          806  GAGACC               GGTCTC(1/5)
BsmI          179  GCATTC               GAATGC(1/-1)
BspHI        1010  TCATGA               T^CATGA
BssSI         481  CACGAG               CACGAG(-5/-1)
BstUI        1085  CGCG                 CG^CG
EarI          196  CTCTTC               CTCTTC(1/4)
Ksp632I       196  CTCTTC               CTCTTC(1/4)
KspI         1084  CCGCGG               CCGC^GG
MluNI          23  TGGCCA               TGG^CCA
MscI           23  TGGCCA               TGG^CCA
MvnI         1085  CGCG                 CG^CG
NcoI          351  CCATGG               C^CATGG
NspI          898  GCATGC               [GA]CATG^[CT]
RcaI         1010  TCATGA               T^CATGA
SacII        1084  CCGCGG               CCGC^GG
SmaI          728  CCCGGG               CCC^GGG
SmlI          121  CTCAAG               C^T[CT][GA]AG
SphI          898  GCATGC               GCATG^C
TfiI          113  GATTC                G^A[AT]TC
XmaCI         728  CCCGGG               C^CCGGG
XmaI          728  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AfaI          549  GTAC                 GT^AC
              776  GTAC                
Alw44I        688  GTGCAC               G^TGCAC
             1377  GTGCAC              
AocI          616  CCTGAGG              CC^T[ACGT]AGG
             1324  CCTGAGG             
ApaLI         688  GTGCAC               G^TGCAC
             1377  GTGCAC              
BbsI          370  GTCTTC               GAAGAC(2/6)
              984  GTCTTC              
BlpI          138  GCTCAGC              GC^T[ACGT]AGC
              336  GCTCAGC             
Bpu1102I      138  GCTCAGC              GC^T[ACGT]AGC
              336  GCTCAGC             
BpuAI         370  GTCTTC               GAAGAC(2/6)
              984  GTCTTC              
BsgI           17  CTGCAC               GTGCAG(16/14)
              670  CTGCAC              
BsiEI         396  CGGCCG               CG[GA][CT]^CG
             1046  CGGCCG              
BsmAI         268  GAGAC                GTCTC(1/5)
              806  GAGAC               
Bsu36I        616  CCTGAGG              CC^T[ACGT]AGG
             1324  CCTGAGG             
CelII         138  GCTCAGC              GC^T[ACGT]AGC
              336  GCTCAGC             
CfoI          381  GCGC                 GCG^C
              945  GCGC                
EagI          396  CGGCCG               C^GGCCG
             1046  CGGCCG              
EclXI         396  CGGCCG               C^GGCCG
             1046  CGGCCG              
Eco52I        396  CGGCCG               C^GGCCG
             1046  CGGCCG              
Eco81I        616  CCTGAGG              CC^T[ACGT]AGG
             1324  CCTGAGG             
HhaI          381  GCGC                 GCG^C
              945  GCGC                
HinP1I        381  GCGC                 G^CGC
              945  GCGC                
MslI           27  CATGCCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              691  CACCTTGATG          
NaeI          299  GCCGGC               GCC^GGC
              398  GCCGGC              
NgoMIV        299  GCCGGC               G^CCGGC
              398  GCCGGC              
PflMI         678  CCAGGGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1283  CCAGCAACTGG         
PleI          270  GACTC                GAGTC(4/5)
              307  GAGTC               
RsaI          549  GTAC                 GT^AC
              776  GTAC                
Van91I        678  CCAGGGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1283  CCAGCAACTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AciI        AluI        AlwNI       ApaI        AspHI       
AvaI        AvaII       BanI        BanII       BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmFI       BsoBI       Bsp1286I    BspMI       BsrFI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
Cac8I       Cfr10I      Cfr13I      DdeI        DraII       
DsaI        EaeI        EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
MaeI        MaeIII      MboII       MnlI        MspA1I      
MspI        MvaI        MwoI        NciI        NlaIII      
NlaIV       PstI        PvuII       Sau96I      ScrFI       
SfaNI       SfcI        StuI        StyI        TseI        
Tsp45I      TspRI       XcmI        
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