Restriction map for pF1KE0951
##################################
# NO CUT ENZYMES (  116 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AgeI        Alw44I      AlwNI       Aor51HI     
ApaLI       AscI        Asp700I     Asp718I     AvrII       
BalI        BbeI        BbrPI       BbvCI       BcgI        
BclI        BglII       BlnI        BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsmI        BspDI       BspEI       BspMI       
BsrDI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstZ17I     Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EaeI        
EagI        EclXI       Eco47III    Eco52I      EcoRV       
EcoT22I     FbaI        FseI        HaeII       HgaI        
Hin1I       HincII      HindII      HpaI        KasI        
KpnI        KspI        MaeII       MamI        MfeI        
MluI        MluNI       MroI        MscI        MunI        
NaeI        NarI        NdeI        NgoMIV      NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        PvuII       RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    StuI        
SwaI        Van91I      XbaI        XcmI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AccII          75  CGCG                 CG^CG
AflII         976  CTTAAG               C^TTAAG
AflIII        668  ACATGT               A^C[GA][CT]GT
AhdI          667  GACATGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          658  CCTGAGG              CC^T[ACGT]AGG
ApaI         1145  GGGCCC               GGGCC^C
AseI          837  ATTAAT               AT^TAAT
AsnI          837  ATTAAT               AT^TAAT
AspEI         667  GACATGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         586  GTGCTC               G[AT]GC[AT]^C
AspI          823  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI           66  CCCGGG               C^[CT]CG[GA]G
AviII         622  TGCGCA               TGC^GCA
BamHI         849  GGATCC               G^GATCC
BanII        1145  GGGCCC               G[GA]GC[CT]^C
BciVI         930  GGATAC               GTATCC(6/5)
BfrI          976  CTTAAG               C^TTAAG
BglI           55  GCCAGTGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI         1070  GAGACC               GGTCTC(1/5)
BsgI         1214  GTGCAG               GTGCAG(16/14)
BsiHKAI       586  GTGCTC               G[AT]GC[AT]^C
BsmAI        1070  GAGAC                GTCTC(1/5)
BsoBI          66  CCCGGG               C^[CT]CG[GA]G
BspHI         590  TCATGA               T^CATGA
BspLU11I      668  ACATGT               A^CATGT
BsrBI         935  CCGCTC               CCGCTC(-3/-3)
BstEII         48  GGTCACC              G^GT[ACGT]ACC
BstPI          48  GGTCACC              G^GT[ACGT]ACC
BstUI          75  CGCG                 CG^CG
BstYI         849  GGATCC               [GA]^GATC[CT]
Bsu36I        658  CCTGAGG              CC^T[ACGT]AGG
Eam1105I      667  GACATGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        658  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1305  CCTAGCAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I        48  GGTCACC              G^GT[ACGT]ACC
FauI         1010  CCCGC                CCCGC(4/6)
FspI          622  TGCGCA               TGC^GCA
HindIII       973  AAGCTT               A^AGCTT
MflI          849  GGATCC               [GA]^GATC[CT]
MslI          466  CATGAAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        983  CAGCGG               C[AC]G^C[GT]G
MvnI           75  CGCG                 CG^CG
NcoI         1035  CCATGG               C^CATGG
NheI         1063  GCTAGC               G^CTAGC
NspI          668  ACATGT               [GA]CATG^[CT]
PflFI         823  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         199  GGGACCC              [GA]G^G[AT]CC[CT]
PshBI         837  ATTAAT               AT^TAAT
PstI          422  CTGCAG               CTGCA^G
RcaI          590  TCATGA               T^CATGA
SapI           41  GCTCTTC              GCTCTTC(1/4)
SmaI           66  CCCGGG               CCC^GGG
SmlI          976  CTTAAG               C^T[CT][GA]AG
SspI          773  AATATT               AAT^ATT
TfiI          504  GAATC                G^A[AT]TC
Tth111I       823  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII         849  GGATCC               [GA]^GATC[CT]
XmaCI          66  CCCGGG               C^CCGGG
XmaI           66  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

BanI           15  GGTGCC               G^G[CT][GA]CC
             1248  GGTGCC              
BbsI          594  GAAGAC               GAAGAC(2/6)
             1323  GAAGAC              
BlpI          123  GCTGAGC              GC^T[ACGT]AGC
             1174  GCTGAGC             
Bpu1102I      123  GCTGAGC              GC^T[ACGT]AGC
             1174  GCTGAGC             
BpuAI         594  GAAGAC               GAAGAC(2/6)
             1323  GAAGAC              
Bsp1407I      179  TGTACA               T^GTACA
              921  TGTACA              
BsrGI         179  TGTACA               T^GTACA
              921  TGTACA              
BsrI           95  ACTGG                ACTGG(1/-1)
               56  CCAGT               
BstXI         818  CCAAGGACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1253  CCAAGGCAGTGG        
CelII         123  GCTGAGC              GC^T[ACGT]AGC
             1174  GCTGAGC             
CfoI          121  GCGC                 GCG^C
              623  GCGC                
DsaI          892  CCGTGG               C^C[GA][CT]GG
             1035  CCATGG              
EcoRI         758  GAATTC               G^AATTC
              955  GAATTC              
HhaI          121  GCGC                 GCG^C
              623  GCGC                
HinP1I        121  GCGC                 G^CGC
              623  GCGC                
PleI          560  GACTC                GAGTC(4/5)
              145  GAGTC               
SspBI         179  TGTACA               T^GTACA
              921  TGTACA              
TaqI          101  TCGA                 T^CGA
              137  TCGA                
TthHB8I       101  TCGA                 T^CGA
              137  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BbvI        BcnI        BfaI        
BmyI        BpmI        BsaJI       BseRI       BsiYI       
BslI        BsmFI       Bsp1286I    BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EarI        EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MnlI        MseI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       
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