################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BlnI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI MaeII MamI MfeI MluI MroI MslI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI SspBI SspI SwaI TfiI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 244 GTCTAC GT^[AC][GT]AC AccII 861 CGCG CG^CG AcsI 97 GAATTC [GA]^AATT[CT] Alw44I 1213 GTGCAC G^TGCAC AlwI 1012 GGATC GGATC(4/5) AocI 265 CCTGAGG CC^T[ACGT]AGG ApaLI 1213 GTGCAC G^TGCAC ApoI 97 GAATTC [GA]^AATT[CT] AspI 373 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 60 TGGCCA TGG^CCA BglI 846 GCCAGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 917 AGATCT A^GATCT BlpI 177 GCTCAGC GC^T[ACGT]AGC Bpu1102I 177 GCTCAGC GC^T[ACGT]AGC BseRI 118 GAGGAG GAGGAG(10/8) BsmI 1124 GCATTC GAATGC(1/-1) BspMI 651 GCAGGT ACCTGC(4/8) BsrBI 228 GAGCGG CCGCTC(-3/-3) BstUI 861 CGCG CG^CG BstYI 917 AGATCT [GA]^GATC[CT] Bsu36I 265 CCTGAGG CC^T[ACGT]AGG CelII 177 GCTCAGC GC^T[ACGT]AGC Eco81I 265 CCTGAGG CC^T[ACGT]AGG EcoRI 97 GAATTC G^AATTC HgaI 15 GACGC GACGC(5/10) HinfI 1146 GAGTC G^A[ACGT]TC KspI 860 CCGCGG CCGC^GG MflI 917 AGATCT [GA]^GATC[CT] MluNI 60 TGGCCA TGG^CCA MscI 60 TGGCCA TGG^CCA MseI 701 TTAA T^TAA MvnI 861 CGCG CG^CG PflFI 373 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1192 CCATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1146 GAGTC GAGTC(4/5) PvuII 37 CAGCTG CAG^CTG SacII 860 CCGCGG CCGC^GG SapI 694 GAAGAGC GCTCTTC(1/4) SbfI 556 CCTGCAGG CCTGCA^GG SfaNI 112 GATGC GCATC(5/9) SmaI 947 CCCGGG CCC^GGG Sse8387I 556 CCTGCAGG CCTGCA^GG StuI 614 AGGCCT AGG^CCT TaqI 1246 TCGA T^CGA Tru9I 701 TTAA T^TAA Tsp45I 13 GTGAC ^GT[GC]AC Tth111I 373 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1246 TCGA T^CGA Van91I 1192 CCATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 917 AGATCT [GA]^GATC[CT] XmaCI 947 CCCGGG C^CCGGG XmaI 947 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AfaI 195 GTAC GT^AC 456 GTAC ApaI 754 GGGCCC GGGCC^C 1270 GGGCCC BanII 754 GGGCCC G[GA]GC[CT]^C 1270 GGGCCC BsgI 407 GTGCAG GTGCAG(16/14) 548 GTGCAG BsmAI 379 GTCTC GTCTC(1/5) 495 GTCTC BsrDI 1152 CATTGC GCAATG(2/0) 56 GCAATG BsrFI 637 GCCGGC [GA]^CCGG[CT] 1047 GCCGGC BstAPI 24 GCAGTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 550 GCAGCTCCTGC Cfr10I 637 GCCGGC [GA]^CCGG[CT] 1047 GCCGGC DpnII 918 GATC ^GATC 1013 GATC DsaI 860 CCGCGG C^C[GA][CT]GG 1253 CCGTGG MaeIII 13 GTGAC ^GT[ACGT]AC 319 GTTAC MboI 918 GATC ^GATC 1013 GATC MspA1I 37 CAGCTG C[AC]G^C[GT]G 860 CCGCGG NaeI 637 GCCGGC GCC^GGC 1047 GCCGGC NdeII 918 GATC ^GATC 1013 GATC NgoMIV 637 GCCGGC G^CCGGC 1047 GCCGGC NspI 357 ACATGC [GA]CATG^[CT] 458 ACATGC PpuMI 950 GGGACCT [GA]G^G[AT]CC[CT] 993 GGGACCC RsaI 195 GTAC GT^AC 456 GTAC Sau3AI 918 GATC ^GATC 1013 GATC Tsp509I 98 AATT ^AATT 166 AATT XcmI 329 CCATGCCAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 758 CCAAGGAAGAGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AluI AlwNI AspHI AvaI AvaII BbvI BcnI BfaI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI Ksp632I MaeI MboII MnlI MspI MvaI MwoI NciI NlaIII NlaIV PstI Sau96I ScrFI SfcI StyI TseI TspRI