Restriction map for pF1KE0930
##################################
# NO CUT ENZYMES (  165 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BcnI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        ClaI        
CpoI        DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoT22I     
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeII       
MamI        MfeI        MluI        MluNI       MroI        
MscI        MslI        MunI        MvnI        NarI        
NciI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AvaI         1082  CCCGAG               C^[CT]CG[GA]G
BclI          342  TGATCA               T^GATCA
BfaI          140  CTAG                 C^TAG
BsaI         1090  GAGACC               GGTCTC(1/5)
BsgI           68  CTGCAC               GTGCAG(16/14)
BsoBI        1082  CCCGAG               C^[CT]CG[GA]G
BsrFI         612  GCCGGC               [GA]^CCGG[CT]
Cfr10I        612  GCCGGC               [GA]^CCGG[CT]
DraI          378  TTTAAA               TTT^AAA
EarI          362  GAAGAG               CTCTTC(1/4)
EcoRV         868  GATATC               GAT^ATC
EcoT14I       981  CCAAGG               C^C[AT][AT]GG
FbaI          342  TGATCA               T^GATCA
HapII         613  CCGG                 C^CGG
HpaII         613  CCGG                 C^CGG
Ksp632I       362  GAAGAG               CTCTTC(1/4)
MaeI          140  CTAG                 C^TAG
MspI          613  CCGG                 C^CGG
NaeI          612  GCCGGC               GCC^GGC
NgoMIV        612  GCCGGC               G^CCGGC
NlaIII        742  CATG                 CATG^
ScaI          756  AGTACT               AGT^ACT
SexAI         566  ACCTGGT              A^CC[AT]GGT
SfcI          544  CTACAG               C^T[GA][CT]AG
SmlI          210  CTTGAG               C^T[CT][GA]AG
StyI          981  CCAAGG               C^C[AT][AT]GG
TaqI          582  TCGA                 T^CGA
TthHB8I       582  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcsI          145  AAATTT               [GA]^AATT[CT]
              933  AAATTT              
AlwI          738  GATCC                GGATC(4/5)
              813  GGATC               
AlwNI         469  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1024  CAGTTCCTG           
ApaI          521  GGGCCC               GGGCC^C
              606  GGGCCC              
ApoI          145  AAATTT               [GA]^AATT[CT]
              933  AAATTT              
AvaII         117  GGTCC                G^G[AT]CC
              880  GGACC               
BanI          837  GGTGCC               G^G[CT][GA]CC
              952  GGTGCC              
BanII         521  GGGCCC               G[GA]GC[CT]^C
              606  GGGCCC              
BmyI          521  GGGCCC               G[AGT]GC[ACT]^C
              606  GGGCCC              
BseRI        1143  CTCCTC               GAGGAG(10/8)
             1132  GAGGAG              
Bsp1286I      521  GGGCCC               G[AGT]GC[ACT]^C
              606  GGGCCC              
BsrDI          76  GCAATG               GCAATG(2/0)
              394  GCAATG              
BstF5I        767  GGATG                GGATG(2/0)
              996  GGATG               
BstYI         737  AGATCC               [GA]^GATC[CT]
              813  GGATCT              
FauI          609  CCCGC                CCCGC(4/6)
              519  GCGGG               
FokI          767  GGATG                GGATG(9/13)
              996  GGATG               
MflI          737  AGATCC               [GA]^GATC[CT]
              813  GGATCT              
MseI          379  TTAA                 T^TAA
              892  TTAA                
MspA1I        526  CAGCTG               C[AC]G^C[GT]G
              634  CAGCTG              
PpuMI         116  GGGTCCC              [GA]G^G[AT]CC[CT]
              879  AGGACCT             
PvuII         526  CAGCTG               CAG^CTG
              634  CAGCTG              
Tru9I         379  TTAA                 T^TAA
              892  TTAA                
Tsp45I        695  GTGAC                ^GT[GC]AC
             1043  GTCAC               
XhoII         737  AGATCC               [GA]^GATC[CT]
              813  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AciI        AfaI        AluI        BbvI        BpmI        
BsaJI       BsmAI       BsmFI       BsrI        BssKI       
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EcoO109I    EcoRII      Fnu4HI      HaeIII      
HinfI       HphI        ItaI        MaeIII      MboI        
MboII       MnlI        MvaI        MwoI        NdeII       
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       TfiI        TseI        Tsp509I     
TspRI       
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