Restriction map for pF1KE0916
 (Circular) (Six-base) MAPSORT of: pF1KE0916.seq  Check: 669  from: 1  to: 3974



pF1KE0916  3974 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AccI GT'mk_AC

Cuts at:    434    1012     434 
   Size:        578    3396
 
Acc65I G'GTAC_C

Cuts at:    990     990 
   Size:       3974
 
AclI AA'CG_TT

Cuts at:   3814    3814 
   Size:       3974
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1799    2231    2978    3246    1799 
   Size:        432     747     268    2527

  Fragments arranged by size: 

                2527     747     432     268
 
AfeI AGC'GCT

Cuts at:    493     493 
   Size:       3974
 
AflIII A'CryG_T

Cuts at:   1326    2430    3407    1326 
   Size:       1104     977    1893

  Fragments arranged by size: 

                1893    1104     977
 
AgeI A'CCGG_T

Cuts at:   2362    2362 
   Size:       3974
 
AlwNI CAG_nnn'CTG

Cuts at:    633    1279    2846     633 
   Size:        646    1567    1761

  Fragments arranged by size: 

                1761    1567     646
 
ApaI G_GGCC'C

Cuts at:    598     598 
   Size:       3974
 
ApaLI G'TGCA_C

Cuts at:   2744    2744 
   Size:       3974
 
ApoI r'AATT_y

Cuts at:    978    3327    3583     978 
   Size:       2349     256    1369

  Fragments arranged by size: 

                2349    1369     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3974
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3974
 
AvaI C'yCGr_G

Cuts at:    622     741     825     994     622 
   Size:        119      84     169    3602

  Fragments arranged by size: 

                3602     169     119      84
 
BamHI G'GATC_C

Cuts at:    999     999 
   Size:       3974
 
BanI G'GyrC_C

Cuts at:    990    1404    1652    1687     990 
   Size:        414     248      35    3277

  Fragments arranged by size: 

                3277     414     248      35
 
BanII G_rGCy'C

Cuts at:    581     598     988    2018     581 
   Size:         17     390    1030    2537

  Fragments arranged by size: 

                2537    1030     390      17
 
BbeI G_GCGC'C

Cuts at:   1408    1656    1408 
   Size:        248    3726
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    3940
 
BciVI GTATCCnnnnn_n'

Cuts at:   1872    2633    3974    1872 
   Size:        761    1341    1872

  Fragments arranged by size: 

                1872    1341     761
 
BclI T'GATC_A

Cuts at:    278    1494     278 
   Size:       1216    2758
 
BglII A'GATC_T

Cuts at:   1489    3526    1489 
   Size:       2037    1937
 
BlpI GC'TnA_GC

Cuts at:    292    1086     292 
   Size:        794    3180
 
Bme1580I G_kGCm'C

Cuts at:    598    1599    1692    2748     598 
   Size:       1001      93    1056    1824

  Fragments arranged by size: 

                1824    1056    1001      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1186    1267    1602    3746    1186 
   Size:         81     335    2144    1414

  Fragments arranged by size: 

                2144    1414     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2341    2341 
   Size:       3974
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    244     592     895     951     244 
   Size:        348     303      56    3267

  Fragments arranged by size: 

                3267     348     303      56
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1150    2029    2521    2819    1150 
   Size:        879     492     298    2305

  Fragments arranged by size: 

                2305     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3678    3678 
   Size:       3974
 
BsaAI yAC'GTr

Cuts at:    176     284     758    1327     176 
   Size:        108     474     569    2823

  Fragments arranged by size: 

                2823     569     474     108
 
BsaBI GATnn'nnATC

Cuts at:      6     241    1488    3525       6 
   Size:        235    1247    2037     455

  Fragments arranged by size: 

                2037    1247     455     235
 
BsaHI Gr'CG_yC

Cuts at:   1405    1653    2355    3851    1405 
   Size:        248     702    1496    1528

  Fragments arranged by size: 

                1528    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1163    1684    2362    2636    2783    3194    1163 
   Size:        521     678     274     147     411    1943

  Fragments arranged by size: 

                1943     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    211     241     211 
   Size:         30    3944
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    363     589     363 
   Size:        226    3748
 
BseYI C'CCAG_C

Cuts at:    482     711     730    1399    2157    2734    3230     482 
   Size:        229      19     669     758     577     496    1226

  Fragments arranged by size: 

                1226     758     669     577     496     229      19
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    156     296     585     156 
   Size:        140     289    3545

  Fragments arranged by size: 

                3545     289     140
 
BsiEI CG_ry'CG

Cuts at:    104     459    1516    1562    2770     104 
   Size:        355    1057      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1057     355      46
 
BsiHKAI G_wGCw'C

Cuts at:    400     988    1766    1956    2748     400 
   Size:        588     778     190     792    1626

  Fragments arranged by size: 

                1626     792     778     588     190
 
Bsp1286I G_dGCh'C

Cuts at:    400     581     598     988    1599    1692    1766    1956    2018 
   Size:        181      17     390     611      93      74     190      62

Cuts at:   2018    2748     400 
   Size:        730    1626

  Fragments arranged by size: 

                1626     730     611     390     190     181      93      74
                  62      17
 
BspEI T'CCGG_A

Cuts at:   3194    3194 
   Size:       3974
 
BspHI T'CATG_A

Cuts at:   3150    3150 
   Size:       3974
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    420     533     551    1024    1238    1540    1921     420 
   Size:        113      18     473     214     302     381    2473

  Fragments arranged by size: 

                2473     473     381     302     214     113      18
 
BsrBI CCG'CTC

Cuts at:     50    2266    2320    3804    3969      50 
   Size:       2216      54    1484     165      55

  Fragments arranged by size: 

                2216    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1886    1886 
   Size:       3974
 
BsrFI r'CCGG_y

Cuts at:    329    1972    2153    2362     329 
   Size:       1643     181     209    1941

  Fragments arranged by size: 

                1941    1643     209     181
 
BssHII G'CGCG_C

Cuts at:    657     657 
   Size:       3974
 
BssSI C'ACGA_G

Cuts at:    532    2245    2603     532 
   Size:       1713     358    1903

  Fragments arranged by size: 

                1903    1713     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1351    1351 
   Size:       3974
 
BstBI TT'CG_AA

Cuts at:   2335    2335 
   Size:       3974
 
BstYI r'GATC_y

Cuts at:      1     999    1466    1489    1824    2070    3082    3168    3180 
   Size:        998     467      23     335     246    1012      86      12

Cuts at:   3180    3526       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     998     467     449     346     335     246      86
                  23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    888    1924    2105    2268     888 
   Size:       1036     181     163    2594

  Fragments arranged by size: 

                2594    1036     181     163
 
BtsI GCAGTG_nn'

Cuts at:    861    1217     861 
   Size:        356    3618
 
DraI TTT'AAA

Cuts at:    973     973 
   Size:       3974
 
DrdI GACnn_nn'nnGTC

Cuts at:   1680    2538    1680 
   Size:        858    3116
 
EaeI y'GGCC_r

Cuts at:    464     816    1559    1733    2124    2151    3496     464 
   Size:        352     743     174     391      27    1345     942

  Fragments arranged by size: 

                1345     942     743     391     352     174      27
 
EagI C'GGCC_G

Cuts at:   1559    1559 
   Size:       3974
 
EarI CTCTTCn'nnn_

Cuts at:   1997    2207    1997 
   Size:        210    3764
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    360    2182    2492    2638    3784     360 
   Size:       1822     310     146    1146     550

  Fragments arranged by size: 

                1822    1146     550     310     146
 
EcoICRI GAG'CTC

Cuts at:    986     986 
   Size:       3974
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    244     592     895     951    1799    2231    2978    3246     244 
   Size:        348     303      56     848     432     747     268     972

  Fragments arranged by size: 

                 972     848     747     432     348     303     268      56
            
 
EcoO109I rG'GnC_Cy

Cuts at:    594     595     618     830     881    1113     594 
   Size:          1      23     212      51     232    3455

  Fragments arranged by size: 

                3455     232     212      51      23       1
 
EcoRI G'AATT_C

Cuts at:    978     978 
   Size:       3974
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1993    1993 
   Size:       3974
 
FspI TGC'GCA

Cuts at:   1755    1755 
   Size:       3974
 
HaeII r_GCGC'y

Cuts at:    495     755    1408    1656    2678    3657     495 
   Size:        260     653     248    1022     979     812

  Fragments arranged by size: 

                1022     979     812     653     260     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    349     381     950     982    1816    1846    1848    1878     349 
   Size:         32     569      32     834      30       2      30    2445

  Fragments arranged by size: 

                2445     834     569      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    129    1013     129 
   Size:        884    3090
 
KasI G'GCGC_C

Cuts at:   1404    1652    1404 
   Size:        248    3726
 
KpnI G_GTAC'C

Cuts at:    994     994 
   Size:       3974
 
MluI A'CGCG_T

Cuts at:   3407    3407 
   Size:       3974
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2645    2829    2645 
   Size:        184    3790
 
MscI TGG'CCA

Cuts at:   1735    1735 
   Size:       3974
 
MslI CAynn'nnrTG

Cuts at:    247     250    1963    2090     247 
   Size:          3    1713     127    2131

  Fragments arranged by size: 

                2131    1713     127       3
 
MspA1I CmG'CkG

Cuts at:    486     633     770    1085    1399    1759    2772    3017     486 
   Size:        147     137     315     314     360    1013     245    1443

  Fragments arranged by size: 

                1443    1013     360     315     314     245     147     137
            
 
NaeI GCC'GGC

Cuts at:    331    2155     331 
   Size:       1824    2150
 
NarI GG'CG_CC

Cuts at:   1405    1653    1405 
   Size:        248    3726
 
NgoMIV G'CCGG_C

Cuts at:    329    2153     329 
   Size:       1824    2150
 
NspI r_CATG'y

Cuts at:   1027    1527    2434    1027 
   Size:        500     907    2567

  Fragments arranged by size: 

                2567     907     500
 
PciI A'CATG_T

Cuts at:   2430    2430 
   Size:       3974
 
PflMI CCAn_nnn'nTGG

Cuts at:    207     207 
   Size:       3974
 
PmeI GTTT'AAAC

Cuts at:    973     973 
   Size:       3974
 
PmlI CAC'GTG

Cuts at:    176     284     758     176 
   Size:        108     474    3392

  Fragments arranged by size: 

                3392     474     108
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3113    3145    3113 
   Size:         32    3942
 
PpuMI rG'GwC_Cy

Cuts at:    618     830     881     618 
   Size:        212      51    3711

  Fragments arranged by size: 

                3711     212      51
 
PspOMI G'GGCC_C

Cuts at:    594     594 
   Size:       3974
 
PstI C_TGCA'G

Cuts at:    530    1021    1706     530 
   Size:        491     685    2798

  Fragments arranged by size: 

                2798     685     491
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3974
 
PvuII CAG'CTG

Cuts at:    633    1399    1759     633 
   Size:        766     360    2848

  Fragments arranged by size: 

                2848     766     360
 
RsrII CG'GwC_CG

Cuts at:   2169    2169 
   Size:       3974
 
SacI G_AGCT'C

Cuts at:    988     988 
   Size:       3974
 
SalI G'TCGA_C

Cuts at:   1011    1011 
   Size:       3974
 
SanDI GG'GwC_CC

Cuts at:    618     881     618 
   Size:        263    3711
 
SapI GCTCTTCn'nnn_

Cuts at:   1997    2207    1997 
   Size:        210    3764
 
SbfI CC_TGCA'GG

Cuts at:   1021    1021 
   Size:       3974
 
ScaI AGT'ACT

Cuts at:   3491    3491 
   Size:       3974
 
SfcI C'TryA_G

Cuts at:     33     526     801    1017    1702    2695    2886      33 
   Size:        493     275     216     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     493     275     216     191
 
SfoI GGC'GCC

Cuts at:   1406    1654    1406 
   Size:        248    3726
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3974
 
SmaI CCC'GGG

Cuts at:    827     996     827 
   Size:        169    3805
 
SmlI C'TyrA_G

Cuts at:   1129    2044    2536    2798    1129 
   Size:        915     492     262    2305

  Fragments arranged by size: 

                2305     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3974
 
SphI G_CATG'C

Cuts at:   1027    1527    1027 
   Size:        500    3474
 
StyI C'CwwG_G

Cuts at:   1108    1462    1108 
   Size:        354    3620
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1557    1690    2149    2358    1557 
   Size:        133     459     209    3173

  Fragments arranged by size: 

                3173     459     209     133
 
TatI w'GTAC_w

Cuts at:   3489    3489 
   Size:       3974
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3491    3523    3491 
   Size:         32    3942
 
Tth111I GACn'n_nGTC

Cuts at:   1771    1771 
   Size:       3974
 
XbaI T'CTAG_A

Cuts at:   1005    1005 
   Size:       3974
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    459     853    1469     459 
   Size:        394     616    2964

  Fragments arranged by size: 

                2964     616     394
 
XmaI C'CCGG_G

Cuts at:    825     994     825 
   Size:        169    3805
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI    BstYI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI   PspOMI     PstI     PvuI
    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AleI     AlfI     AloI     AscI
    AvrII     BaeI     BbsI    BbvCI    BfrBI     BglI    BmgBI     BmtI
   Bpu10I    BsiWI     BsmI    BsmBI    BsrGI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PfoI    PshAI     PsiI    PspXI
     PsrI    SacII    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     StuI     SwaI     XhoI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com