Restriction map for pF1KE0915
 (Linear) (Six-base) MAPSORT of: KIEE0915.seq  Check: 2130  from: 1  to: 789



KIEE0915.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AgeI A'CCGG_T

Cuts at:      0      33     789 
   Size:         33     756
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     345     789 
   Size:        345     444
 
AlwNI CAG_nnn'CTG

Cuts at:      0     338     789 
   Size:        338     451
 
BamHI G'GATC_C

Cuts at:      0     728     789 
   Size:        728      61
 
BanI G'GyrC_C

Cuts at:      0     304     469     789 
   Size:        304     165     320

  Fragments arranged by size: 

                 320     304     165
 
BfrBI ATG'CAT

Cuts at:      0     580     789 
   Size:        580     209
 
BglII A'GATC_T

Cuts at:      0      75     789 
   Size:         75     714
 
Bme1580I G_kGCm'C

Cuts at:      0     472     789 
   Size:        472     317
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     213     723     789 
   Size:        213     510      66

  Fragments arranged by size: 

                 510     213      66
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      18     789 
   Size:         18     771
 
BsaAI yAC'GTr

Cuts at:      0     516     711     789 
   Size:        516     195      78

  Fragments arranged by size: 

                 516     195      78
 
BsaBI GATnn'nnATC

Cuts at:      0     283     789 
   Size:        283     506
 
BsaWI w'CCGG_w

Cuts at:      0      33     533     789 
   Size:         33     500     256

  Fragments arranged by size: 

                 500     256      33
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      90     120     200     230     241     271     680     710 
   Size:         90      30      80      30      11      30     409      30

Cuts at:    710     789 
   Size:         79

  Fragments arranged by size: 

                 409      90      80      79      30      30      30      30
                  11
 
BseYI C'CCAG_C

Cuts at:      0     134     313     485     789 
   Size:        134     179     172     304

  Fragments arranged by size: 

                 304     179     172     134
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     713     789 
   Size:         92     621      76

  Fragments arranged by size: 

                 621      92      76
 
Bsp1286I G_dGCh'C

Cuts at:      0     472     789 
   Size:        472     317
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     305     789 
   Size:        305     484
 
BsrDI GCAATG_nn'

Cuts at:      0     251     367     789 
   Size:        251     116     422

  Fragments arranged by size: 

                 422     251     116
 
BsrFI r'CCGG_y

Cuts at:      0      33     789 
   Size:         33     756
 
BstBI TT'CG_AA

Cuts at:      0     381     789 
   Size:        381     408
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      18     789 
   Size:         18     771
 
BstYI r'GATC_y

Cuts at:      0      75      84     728     789 
   Size:         75       9     644      61

  Fragments arranged by size: 

                 644      75      61       9
 
BtgI C'CryG_G

Cuts at:      0     204     308     464     789 
   Size:        204     104     156     325

  Fragments arranged by size: 

                 325     204     156     104
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      65     789 
   Size:         65     724
 
EaeI y'GGCC_r

Cuts at:      0     122     789 
   Size:        122     667
 
EarI CTCTTCn'nnn_

Cuts at:      0     143     789 
   Size:        143     646
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     190     789 
   Size:        190     599
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     213     723     789 
   Size:        213     510      66

  Fragments arranged by size: 

                 510     213      66
 
EcoO109I rG'GnC_Cy

Cuts at:      0     499     789 
   Size:        499     290
 
HaeII r_GCGC'y

Cuts at:      0       7     789 
   Size:          7     782
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     667     680     699     712     789 
   Size:        667      13      19      13      77

  Fragments arranged by size: 

                 667      77      19      13      13
 
HincII GTy'rAC

Cuts at:      0     699     789 
   Size:        699      90
 
MspA1I CmG'CkG

Cuts at:      0     223     241     325     409     789 
   Size:        223      18      84      84     380

  Fragments arranged by size: 

                 380     223      84      84      18
 
NcoI C'CATG_G

Cuts at:      0     204     308     464     789 
   Size:        204     104     156     325

  Fragments arranged by size: 

                 325     204     156     104
 
NdeI CA'TA_TG

Cuts at:      0     582     789 
   Size:        582     207
 
NsiI A_TGCA'T

Cuts at:      0     582     789 
   Size:        582     207
 
PmlI CAC'GTG

Cuts at:      0     711     789 
   Size:        711      78
 
PvuII CAG'CTG

Cuts at:      0     223     409     789 
   Size:        223     186     380

  Fragments arranged by size: 

                 380     223     186
 
SapI GCTCTTCn'nnn_

Cuts at:      0     143     789 
   Size:        143     646
 
SfcI C'TryA_G

Cuts at:      0     236     401     557     669     789 
   Size:        236     165     156     112     120

  Fragments arranged by size: 

                 236     165     156     120     112
 
StuI AGG'CCT

Cuts at:      0     631     789 
   Size:        631     158
 
StyI C'CwwG_G

Cuts at:      0     204     308     464     789 
   Size:        204     104     156     325

  Fragments arranged by size: 

                 325     204     156     104
 
TatI w'GTAC_w

Cuts at:      0     443     789 
   Size:        443     346
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      90     122     789 
   Size:         90      32     667

  Fragments arranged by size: 

                 667      90      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     747     789 
   Size:        747      42
 
XmnI GAAnn'nnTTC

Cuts at:      0     167     789 
   Size:        167     622
 

 Enzymes that do cut:

     AgeI     AlfI    AlwNI    BamHI     BanI    BfrBI    BglII Bme1580I
     BpmI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseYI     BsgI
 Bsp1286I    BspMI    BsrDI    BsrFI    BstBI    BstXI    BstYI     BtgI
     DrdI     EaeI     EarI     EciI  Eco57MI EcoO109I    HaeII    Hin4I
   HincII   MspA1I     NcoI     NdeI     NsiI     PmlI    PvuII     SapI
     SfcI     StuI     StyI     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI     BglI     BlpI    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI    BsaHI    BseRI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrGI   BssHII
    BssSI   BstAPI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     EagI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI   NgoMIV
     NheI     NotI     NruI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII
  Tth111I     XbaI     XhoI     XmaI     ZraI
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