Restriction map for
pF1KE0738
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BamHI BbeI
BbrPI BcgI BciVI BclI BfaI
BfrI BlnI BsaAI BsaBI BsaHI
BsaWI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoRI EcoRV
EcoT22I FbaI FseI Hin1I HincII
HindII HindIII HpaI KasI KpnI
MaeI MaeII MamI MfeI MluI
MroI MseI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
ScaI SexAI SfoI SfuI SmaI
SnaBI SpeI SspBI SspI SwaI
Tru9I Tsp509I Tth111I Van91I XbaI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 1040 CGCG CG^CG
AfaI 333 GTAC GT^AC
Aor51HI 194 AGCGCT AGC^GCT
ApaI 303 GGGCCC GGGCC^C
AspHI 343 GTGCTC G[AT]GC[AT]^C
AviII 419 TGCGCA TGC^GCA
BbsI 505 GTCTTC GAAGAC(2/6)
BglI 179 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 674 AGATCT A^GATCT
BlpI 345 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 345 GCTCAGC GC^T[ACGT]AGC
BpuAI 505 GTCTTC GAAGAC(2/6)
BsaI 1258 GAGACC GGTCTC(1/5)
BsiEI 143 CGACCG CG[GA][CT]^CG
BsiHKAI 343 GTGCTC G[AT]GC[AT]^C
BsmAI 1258 GAGAC GTCTC(1/5)
BsmI 924 GAATGC GAATGC(1/-1)
BsrBI 1143 CCGCTC CCGCTC(-3/-3)
BsrI 441 CCAGT ACTGG(1/-1)
BstEII 633 GGTGACC G^GT[ACGT]ACC
BstPI 633 GGTGACC G^GT[ACGT]ACC
BstUI 1040 CGCG CG^CG
CelII 345 GCTCAGC GC^T[ACGT]AGC
Eco47III 194 AGCGCT AGC^GCT
EcoNI 519 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 633 GGTGACC G^GT[ACGT]ACC
FspI 419 TGCGCA TGC^GCA
KspI 1039 CCGCGG CCGC^GG
MvnI 1040 CGCG CG^CG
NaeI 1091 GCCGGC GCC^GGC
NgoMIV 1091 GCCGGC G^CCGGC
PaeR7I 1116 CTCGAG C^TCGAG
PpuMI 1071 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 846 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 337 CTGCAG CTGCA^G
RsaI 333 GTAC GT^AC
SacII 1039 CCGCGG CCGC^GG
SapI 252 GCTCTTC GCTCTTC(1/4)
SbfI 336 CCTGCAGG CCTGCA^GG
SfiI 178 GGCCAGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1090 CGCCGGCG C[GA]^CCGG[CT]G
SphI 203 GCATGC GCATG^C
Sse8387I 336 CCTGCAGG CCTGCA^GG
TaqI 1117 TCGA T^CGA
TthHB8I 1117 TCGA T^CGA
XcmI 268 CCACAGCTGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1116 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 14 enzymes) #
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BanI 43 GGCACC G^G[CT][GA]CC
1249 GGCACC
BseRI 91 GAGGAG GAGGAG(10/8)
719 GAGGAG
BsgI 220 CTGCAC GTGCAG(16/14)
318 GTGCAG
BsrFI 630 GCCGGT [GA]^CCGG[CT]
1091 GCCGGC
Cfr10I 630 GCCGGT [GA]^CCGG[CT]
1091 GCCGGC
EarI 253 CTCTTC CTCTTC(1/4)
1210 GAAGAG
HaeII 194 AGCGCT [GA]GCGC^[CT]
249 GGCGCT
HgaI 384 GACGC GACGC(5/10)
1050 GCGTC
Ksp632I 253 CTCTTC CTCTTC(1/4)
1210 GAAGAG
PleI 116 GAGTC GAGTC(4/5)
1213 GAGTC
SfcI 337 CTGCAG C^T[GA][CT]AG
654 CTACAG
SmlI 500 CTCAAG C^T[CT][GA]AG
1116 CTCGAG
StuI 704 AGGCCT AGG^CCT
1204 AGGCCT
TfiI 4 GATTC G^A[AT]TC
940 GATTC
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaI
AvaII BalI BanII BbvCI BbvI
BcnI BmyI BpmI BsaJI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PvuII
Sau3AI Sau96I ScrFI SfaNI StyI
TseI Tsp45I TspRI XhoII