Restriction map for pF1KE0751
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AfaI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspLU11I    BspMI       BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoT22I     FbaI        FseI        FspI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MaeI        MamI        
MfeI        MluNI       MscI        MseI        MunI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        SwaI        
Tru9I       Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccIII        877  TCCGGA               T^CCGGA
AcsI          127  AAATTT               [GA]^AATT[CT]
AflIII        623  ACGCGT               A^C[GA][CT]GT
AlwNI         836  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          596  GGGCCC               GGGCC^C
ApoI          127  AAATTT               [GA]^AATT[CT]
BanI           22  GGCACC               G^G[CT][GA]CC
BciVI         976  GGATAC               GTATCC(6/5)
BlpI          553  GCTCAGC              GC^T[ACGT]AGC
BpmI          180  CTCCAG               CTGGAG(16/14)
Bpu1102I      553  GCTCAGC              GC^T[ACGT]AGC
BsaWI         877  TCCGGA               [AT]^CCGG[AT]
BseAI         877  TCCGGA               T^CCGGA
BsmAI         113  GAGAC                GTCTC(1/5)
BspEI         877  TCCGGA               T^CCGGA
BspHI         155  TCATGA               T^CATGA
BsrDI         415  GCAATG               GCAATG(2/0)
BsrI          751  CCAGT                ACTGG(1/-1)
BstF5I        644  GGATG                GGATG(2/0)
BstXI         659  CCAACTTCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         162  AGATCC               [GA]^GATC[CT]
CelII         553  GCTCAGC              GC^T[ACGT]AGC
DraII         837  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO109I      837  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRV         653  GATATC               GAT^ATC
FauI          344  GCGGG                CCCGC(4/6)
FokI          644  GGATG                GGATG(9/13)
HaeII          34  AGCGCC               [GA]GCGC^[CT]
HgaI          622  GACGC                GACGC(5/10)
HphI           97  TCACC                GGTGA(8/7)
MaeII         202  ACGT                 A^CGT
MflI          162  AGATCC               [GA]^GATC[CT]
MluI          623  ACGCGT               A^CGCGT
MroI          877  TCCGGA               T^CCGGA
NcoI          617  CCATGG               C^CATGG
PflMI         703  CCAAGCTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         837  AGGACCT              [GA]G^G[AT]CC[CT]
RcaI          155  TCATGA               T^CATGA
TspRI         752  CAGTG                CA[GC]TG[ACGT][ACGT]^
Van91I        703  CCAAGCTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          933  CCACGGCATAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         162  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccII         624  CGCG                 CG^CG
              803  CGCG                
AlwI          163  GATCC                GGATC(4/5)
              784  GGATC               
BanII         596  GGGCCC               G[GA]GC[CT]^C
              918  GGGCTC              
BmyI          596  GGGCCC               G[AGT]GC[ACT]^C
              918  GGGCTC              
BsmFI         583  GGGAC                GGGAC(10/14)
              603  GGGAC               
Bsp1286I      596  GGGCCC               G[AGT]GC[ACT]^C
              918  GGGCTC              
BstUI         624  CGCG                 CG^CG
              803  CGCG                
EcoT14I        18  CCAAGG               C^C[AT][AT]GG
              617  CCATGG              
MspA1I        737  CAGCGG               C[AC]G^C[GT]G
              761  CAGCGG              
MvnI          624  CGCG                 CG^CG
              803  CGCG                
PleI           43  GACTC                GAGTC(4/5)
              874  GACTC               
SfaNI         412  GATGC                GCATC(5/9)
              645  GATGC               
SmaI          599  CCCGGG               CCC^GGG
              819  CCCGGG              
SspI          333  AATATT               AAT^ATT
              383  AATATT              
StyI           18  CCAAGG               C^C[AT][AT]GG
              617  CCATGG              
TaqI          297  TCGA                 T^CGA
              389  TCGA                
TfiI          259  GATTC                G^A[AT]TC
              507  GATTC               
TthHB8I       297  TCGA                 T^CGA
              389  TCGA                
XmaCI         599  CCCGGG               C^CCGGG
              819  CCCGGG              
XmaI          599  CCCGGG               C^CCGGG
              819  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AciI        AluI        AvaI        AvaII       BbvI        
BcnI        BsaJI       BsiYI       BslI        BsoBI       
BssKI       BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DsaI        EaeI        EcoRII      
Fnu4HI      HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       ItaI        MaeIII      MboI        
MboII       MnlI        MslI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       TseI        Tsp45I      
Tsp509I     
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