Restriction map for pF1KE0751
 (Linear) (Six-base) MAPSORT of: KIEE0751.seq  Check: 1988  from: 1  to: 984



KIEE0751.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AflIII A'CryG_T

Cuts at:      0     623     984 
   Size:        623     361
 
AleI CACnn'nnGTG

Cuts at:      0     364     984 
   Size:        364     620
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     661     984 
   Size:        661     323
 
AlwNI CAG_nnn'CTG

Cuts at:      0     841     984 
   Size:        841     143
 
ApaI G_GGCC'C

Cuts at:      0     600     984 
   Size:        600     384
 
ApoI r'AATT_y

Cuts at:      0     127     984 
   Size:        127     857
 
AvaI C'yCGr_G

Cuts at:      0      27     599     819     921     984 
   Size:         27     572     220     102      63

  Fragments arranged by size: 

                 572     220     102      63      27
 
BanI G'GyrC_C

Cuts at:      0      22     984 
   Size:         22     962
 
BanII G_rGCy'C

Cuts at:      0     600     922     984 
   Size:        600     322      62

  Fragments arranged by size: 

                 600     322      62
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     970     984 
   Size:        970      14
 
BlpI GC'TnA_GC

Cuts at:      0     554     984 
   Size:        554     430
 
Bme1580I G_kGCm'C

Cuts at:      0     600     984 
   Size:        600     384
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     165     984 
   Size:        165     819
 
BsaWI w'CCGG_w

Cuts at:      0     877     984 
   Size:        877     107
 
BseYI C'CCAG_C

Cuts at:      0      47     213     648     732     984 
   Size:         47     166     435      84     252

  Fragments arranged by size: 

                 435     252     166      84      47
 
Bsp1286I G_dGCh'C

Cuts at:      0     600     922     984 
   Size:        600     322      62

  Fragments arranged by size: 

                 600     322      62
 
BspEI T'CCGG_A

Cuts at:      0     877     984 
   Size:        877     107
 
BspHI T'CATG_A

Cuts at:      0     155     984 
   Size:        155     829
 
BsrDI GCAATG_nn'

Cuts at:      0     422     984 
   Size:        422     562
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     666     984 
   Size:        666     318
 
BstYI r'GATC_y

Cuts at:      0     162     984 
   Size:        162     822
 
BtgI C'CryG_G

Cuts at:      0     617     681     933     984 
   Size:        617      64     252      51

  Fragments arranged by size: 

                 617     252      64      51
 
EaeI y'GGCC_r

Cuts at:      0     678     902     930     984 
   Size:        678     224      28      54

  Fragments arranged by size: 

                 678     224      54      28
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     165     984 
   Size:        165     819
 
EcoO109I rG'GnC_Cy

Cuts at:      0     838     984 
   Size:        838     146
 
EcoRV GAT'ATC

Cuts at:      0     655     984 
   Size:        655     329
 
HaeII r_GCGC'y

Cuts at:      0      38     984 
   Size:         38     946
 
MluI A'CGCG_T

Cuts at:      0     623     984 
   Size:        623     361
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     818     984 
   Size:        818     166
 
MslI CAynn'nnrTG

Cuts at:      0     364     515     643     984 
   Size:        364     151     128     341

  Fragments arranged by size: 

                 364     341     151     128
 
MspA1I CmG'CkG

Cuts at:      0     739     763     984 
   Size:        739      24     221

  Fragments arranged by size: 

                 739     221      24
 
NcoI C'CATG_G

Cuts at:      0     617     984 
   Size:        617     367
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     709     984 
   Size:        709     275
 
PpuMI rG'GwC_Cy

Cuts at:      0     838     984 
   Size:        838     146
 
PspOMI G'GGCC_C

Cuts at:      0     596     984 
   Size:        596     388
 
SmaI CCC'GGG

Cuts at:      0     601     821     984 
   Size:        601     220     163

  Fragments arranged by size: 

                 601     220     163
 
SspI AAT'ATT

Cuts at:      0     335     385     984 
   Size:        335      50     599

  Fragments arranged by size: 

                 599     335      50
 
StyI C'CwwG_G

Cuts at:      0      18     617     984 
   Size:         18     599     367

  Fragments arranged by size: 

                 599     367      18
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     940     984 
   Size:        940      44
 
XmaI C'CCGG_G

Cuts at:      0     599     819     984 
   Size:        599     220     165

  Fragments arranged by size: 

                 599     220     165
 

 Enzymes that do cut:

   AflIII     AleI     AlfI    AlwNI     ApaI     ApoI     AvaI     BanI
    BanII    BciVI     BlpI Bme1580I     BpmI    BsaWI    BseYI Bsp1286I
    BspEI    BspHI    BsrDI    BstXI    BstYI     BtgI     EaeI  Eco57MI
 EcoO109I    EcoRV    HaeII     MluI     MmeI     MslI   MspA1I     NcoI
    PflMI    PpuMI   PspOMI     SmaI     SspI     StyI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI
     BglI    BglII    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRI     FalI     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MscI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SphI     SrfI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmnI     ZraI
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