Restriction map for
pF1KB6591
##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmI Bsp1407I BspEI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
CelII CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FseI FspI HincII HindII
HindIII HpaI KasI KpnI MfeI
MluI MluNI MroI MscI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tsp45I Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 610 GACGTC GACGT^C
AcsI 363 GAATTC [GA]^AATT[CT]
AcyI 610 GACGTC G[GA]^CG[CT]C
AfaI 347 GTAC GT^AC
AflIII 422 ACATGT A^C[GA][CT]GT
AlwNI 78 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 36 GGGCCC GGGCC^C
ApoI 363 GAATTC [GA]^AATT[CT]
BamHI 1095 GGATCC G^GATCC
BanI 592 GGCACC G^G[CT][GA]CC
BclI 248 TGATCA T^GATCA
BfaI 1070 CTAG C^TAG
BsaBI 12 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 610 GACGTC G[GA]^CG[CT]C
BsmAI 755 GTCTC GTCTC(1/5)
BsmBI 754 CGTCTC CGTCTC(1/5)
BspDI 1117 ATCGAT AT^CGAT
BspHI 674 TCATGA T^CATGA
BspLU11I 422 ACATGT A^CATGT
BsrBI 1057 GAGCGG CCGCTC(-3/-3)
BsrDI 471 CATTGC GCAATG(2/0)
BsrFI 124 GCCGGC [GA]^CCGG[CT]
Cfr10I 124 GCCGGC [GA]^CCGG[CT]
ClaI 1117 ATCGAT AT^CGAT
DraIII 484 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 610 GACGTCGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 24 CCGCGG C^C[GA][CT]GG
EaeI 167 CGGCCA [CT]^GGCC[GA]
EarI 670 CTCTTC CTCTTC(1/4)
EcoNI 882 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 363 GAATTC G^AATTC
EcoT14I 585 CCTTGG C^C[AT][AT]GG
FbaI 248 TGATCA T^GATCA
Hin1I 610 GACGTC G[GA]^CG[CT]C
Ksp632I 670 CTCTTC CTCTTC(1/4)
KspI 24 CCGCGG CCGC^GG
MaeI 1070 CTAG C^TAG
MaeII 611 ACGT A^CGT
MaeIII 322 GTTAC ^GT[ACGT]AC
MamI 12 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 296 TTAA T^TAA
NaeI 124 GCCGGC GCC^GGC
NgoMIV 124 GCCGGC G^CCGGC
NspI 422 ACATGT [GA]CATG^[CT]
PpuMI 665 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 674 TCATGA T^CATGA
RsaI 347 GTAC GT^AC
SacII 24 CCGCGG CCGC^GG
SmlI 757 CTCAAG C^T[CT][GA]AG
StyI 585 CCTTGG C^C[AT][AT]GG
TfiI 975 GAATC G^A[AT]TC
Tru9I 296 TTAA T^TAA
TspRI 1040 CACTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 193 GTCTAC GT^[AC][GT]AC
352 GTCTAC
AocI 950 CCTTAGG CC^T[ACGT]AGG
1107 CCTGAGG
AvaI 51 CCCGAG C^[CT]CG[GA]G
785 CCCGAG
BglII 17 AGATCT A^GATCT
680 AGATCT
BpmI 927 CTCCAG CTGGAG(16/14)
145 CTGGAG
BsoBI 51 CCCGAG C^[CT]CG[GA]G
785 CCCGAG
BspMI 43 ACCTGC ACCTGC(4/8)
803 ACCTGC
BsrI 822 ACTGG ACTGG(1/-1)
1135 CCAGT
Bsu36I 950 CCTTAGG CC^T[ACGT]AGG
1107 CCTGAGG
DdeI 951 CTTAG C^T[ACGT]AG
1108 CTGAG
DraII 36 GGGCCCT [GA]G^G[ACGT]CC[CT]
665 GGGACCT
Eco81I 950 CCTTAGG CC^T[ACGT]AGG
1107 CCTGAGG
EcoO109I 36 GGGCCCT [GA]G^G[ACGT]CC[CT]
665 GGGACCT
FauI 151 CCCGC CCCGC(4/6)
26 GCGGG
PvuII 142 CAGCTG CAG^CTG
917 CAGCTG
TaqI 614 TCGA T^CGA
1118 TCGA
Tsp509I 364 AATT ^AATT
402 AATT
TthHB8I 614 TCGA T^CGA
1118 TCGA
XcmI 449 CCATGATGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
818 CCAGACTGGTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
AvaII BanII BbsI BbvI BcnI
BmyI BpuAI BsaJI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DpnII EcoRII Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI TseI
XhoII