Restriction map for
pF1KB6591
(Linear) (Six-base) MAPSORT of: KIBB6591.seq Check: 9452 from: 1 to: 1140
KIBB6591.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AatII G_ACGT'C
Cuts at: 0 614 1140
Size: 614 526
AccI GT'mk_AC
Cuts at: 0 194 353 1140
Size: 194 159 787
Fragments arranged by size:
787 194 159
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1063 1140
Size: 1063 77
AflIII A'CryG_T
Cuts at: 0 422 1140
Size: 422 718
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 565 1140
Size: 565 575
AlwNI CAG_nnn'CTG
Cuts at: 0 83 1140
Size: 83 1057
ApaI G_GGCC'C
Cuts at: 0 40 1140
Size: 40 1100
ApoI r'AATT_y
Cuts at: 0 363 1140
Size: 363 777
AvaI C'yCGr_G
Cuts at: 0 51 785 1140
Size: 51 734 355
Fragments arranged by size:
734 355 51
BamHI G'GATC_C
Cuts at: 0 1095 1140
Size: 1095 45
BanI G'GyrC_C
Cuts at: 0 592 1140
Size: 592 548
BanII G_rGCy'C
Cuts at: 0 40 107 152 1140
Size: 40 67 45 988
Fragments arranged by size:
988 67 45 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 277 614 681 837 1140
Size: 277 337 67 156 303
Fragments arranged by size:
337 303 277 156 67
BclI T'GATC_A
Cuts at: 0 248 1140
Size: 248 892
BglII A'GATC_T
Cuts at: 0 17 680 1140
Size: 17 663 460
Fragments arranged by size:
663 460 17
Bme1580I G_kGCm'C
Cuts at: 0 40 1140
Size: 40 1100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 912 1140
Size: 166 746 228
Fragments arranged by size:
746 228 166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 742 1140
Size: 742 398
BsaBI GATnn'nnATC
Cuts at: 0 16 1140
Size: 16 1124
BsaHI Gr'CG_yC
Cuts at: 0 611 1140
Size: 611 529
BseYI C'CCAG_C
Cuts at: 0 95 1140
Size: 95 1045
BsiHKAI G_wGCw'C
Cuts at: 0 631 904 1069 1140
Size: 631 273 165 71
Fragments arranged by size:
631 273 165 71
BsmBI CGTCTCn'nnnn_
Cuts at: 0 760 1140
Size: 760 380
Bsp1286I G_dGCh'C
Cuts at: 0 40 107 152 631 904 1069 1140
Size: 40 67 45 479 273 165 71
Fragments arranged by size:
479 273 165 71 67 45 40
BspHI T'CATG_A
Cuts at: 0 674 1140
Size: 674 466
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 52 812 1140
Size: 52 760 328
Fragments arranged by size:
760 328 52
BsrBI CCG'CTC
Cuts at: 0 1059 1140
Size: 1059 81
BsrDI GCAATG_nn'
Cuts at: 0 470 1140
Size: 470 670
BsrFI r'CCGG_y
Cuts at: 0 124 1140
Size: 124 1016
BstYI r'GATC_y
Cuts at: 0 17 680 1095 1140
Size: 17 663 415 45
Fragments arranged by size:
663 415 45 17
Bsu36I CC'TnA_GG
Cuts at: 0 951 1108 1140
Size: 951 157 32
Fragments arranged by size:
951 157 32
BtgI C'CryG_G
Cuts at: 0 24 1140
Size: 24 1116
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 930 1140
Size: 930 210
ClaI AT'CG_AT
Cuts at: 0 1118 1140
Size: 1118 22
DraIII CAC_nnn'GTG
Cuts at: 0 489 1140
Size: 489 651
DrdI GACnn_nn'nnGTC
Cuts at: 0 616 1140
Size: 616 524
EaeI y'GGCC_r
Cuts at: 0 167 1140
Size: 167 973
EarI CTCTTCn'nnn_
Cuts at: 0 676 1140
Size: 676 464
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 912 1063 1140
Size: 166 746 151 77
Fragments arranged by size:
746 166 151 77
EcoNI CCTnn'n_nnAGG
Cuts at: 0 886 1140
Size: 886 254
EcoO109I rG'GnC_Cy
Cuts at: 0 37 666 1140
Size: 37 629 474
Fragments arranged by size:
629 474 37
EcoRI G'AATT_C
Cuts at: 0 363 1140
Size: 363 777
HaeII r_GCGC'y
Cuts at: 0 59 83 125 1140
Size: 59 24 42 1015
Fragments arranged by size:
1015 59 42 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 640 672 738 770 1140
Size: 640 32 66 32 370
Fragments arranged by size:
640 370 66 32 32
MslI CAynn'nnrTG
Cuts at: 0 244 265 475 742 883 1140
Size: 244 21 210 267 141 257
Fragments arranged by size:
267 257 244 210 141 21
MspA1I CmG'CkG
Cuts at: 0 26 144 919 1140
Size: 26 118 775 221
Fragments arranged by size:
775 221 118 26
NaeI GCC'GGC
Cuts at: 0 126 1140
Size: 126 1014
NgoMIV G'CCGG_C
Cuts at: 0 124 1140
Size: 124 1016
NspI r_CATG'y
Cuts at: 0 426 1140
Size: 426 714
PciI A'CATG_T
Cuts at: 0 422 1140
Size: 422 718
PfoI T'CCnGG_A
Cuts at: 0 1022 1140
Size: 1022 118
PpuMI rG'GwC_Cy
Cuts at: 0 666 1140
Size: 666 474
PspOMI G'GGCC_C
Cuts at: 0 36 1140
Size: 36 1104
PvuII CAG'CTG
Cuts at: 0 144 919 1140
Size: 144 775 221
Fragments arranged by size:
775 221 144
SacII CC_GC'GG
Cuts at: 0 27 1140
Size: 27 1113
SmlI C'TyrA_G
Cuts at: 0 757 1140
Size: 757 383
StyI C'CwwG_G
Cuts at: 0 585 1140
Size: 585 555
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 373 405 1140
Size: 373 32 735
Fragments arranged by size:
735 373 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 456 825 1140
Size: 456 369 315
Fragments arranged by size:
456 369 315
ZraI GAC'GTC
Cuts at: 0 612 1140
Size: 612 528
Enzymes that do cut:
AatII AccI AcuI AflIII AlfI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbsI BclI BglII Bme1580I
BpmI BpuEI BsaBI BsaHI BseYI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BstYI Bsu36I BtgI
BtgZI ClaI DraIII DrdI EaeI EarI Eco57MI EcoNI
EcoO109I EcoRI HaeII Hin4I MslI MspA1I NaeI NgoMIV
NspI PciI PfoI PpuMI PspOMI PvuII SacII SmlI
StyI TstI XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BcgI BciVI BfrBI BglI BlpI BmgBI BmrI
BmtI BplI Bpu10I BsaI BsaAI BsaWI BsaXI BseRI
BsgI BsiEI BsiWI BsmI BspEI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I BtsI CspCI DraI
EagI EciI EcoICRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI Tth111I XbaI XhoI XmaI XmnI