Restriction map for pF1KB7668
##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AflIII      AgeI        AhdI        AlwI        
AlwNI       AocI        ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       BalI        BamHI       
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfrI        BglII       BpuAI       BsaAI       
BsaBI       BsiWI       BsmI        Bsp1407I    BspDI       
BspHI       BspLU11I    BsrDI       BsrGI       BsrI        
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstYI       BstZ17I     Bsu36I      ClaI        DraI        
DraII       DraIII      DrdI        Eam1105I    EarI        
Eco81I      EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HincII      HindII      
HindIII     HpaI        KpnI        Ksp632I     MamI        
MfeI        MflI        MluI        MluNI       MscI        
MseI        MunI        NcoI        NdeI        NheI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PvuI        RcaI        
SalI        SapI        SbfI        ScaI        SfiI        
SfuI        SmaI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        SwaI        TfiI        Tru9I       
Tsp45I      Tsp509I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccIII        755  TCCGGA               T^CCGGA
Alw44I          7  GTGCAC               G^TGCAC
Aor51HI       972  AGCGCT               AGC^GCT
ApaI         1114  GGGCCC               GGGCC^C
ApaLI           7  GTGCAC               G^TGCAC
AspI          477  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         230  TGCGCA               TGC^GCA
AvrII         729  CCTAGG               C^CTAGG
BbeI          733  GGCGCC               GGCGC^C
BfaI          730  CTAG                 C^TAG
BglI         1067  GCCTGCCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI          729  CCTAGG               C^CTAGG
BlpI          720  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      720  GCTGAGC              GC^T[ACGT]AGC
BsaI          646  GAGACC               GGTCTC(1/5)
BsaWI         755  TCCGGA               [AT]^CCGG[AT]
BseAI         755  TCCGGA               T^CCGGA
BsgI         1081  CTGCAC               GTGCAG(16/14)
BspEI         755  TCCGGA               T^CCGGA
BspMI         271  ACCTGC               ACCTGC(4/8)
BssHII        200  GCGCGC               G^CGCGC
CelII         720  GCTGAGC              GC^T[ACGT]AGC
CpoI          410  CGGACCG              CG^G[AT]CCG
DpnII        1032  GATC                 ^GATC
Eco47III      972  AGCGCT               AGC^GCT
EcoNI         372  CCTCAAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI          230  TGCGCA               TGC^GCA
KasI          733  GGCGCC               G^GCGCC
MaeI          730  CTAG                 C^TAG
MaeIII        284  GTAAC                ^GT[ACGT]AC
MboI         1032  GATC                 ^GATC
MroI          755  TCCGGA               T^CCGGA
NarI          733  GGCGCC               GG^CGCC
NdeII        1032  GATC                 ^GATC
NotI          785  GCGGCCGC             GC^GGCCGC
PflFI         477  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        1160  GACCCCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          445  CTGCAG               CTGCA^G
RsrII         410  CGGACCG              CG^G[AT]CCG
Sau3AI       1032  GATC                 ^GATC
SexAI         224  ACCTGGT              A^CC[AT]GGT
SfoI          733  GGCGCC               GGC^GCC
SgrAI         300  CACCGGCG             C[GA]^CCGG[CT]G
SphI          770  GCATGC               GCATG^C
StuI         1065  AGGCCT               AGG^CCT
Tth111I       477  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcyI          733  GGCGCC               G[GA]^CG[CT]C
              960  GACGCC              
AfaI          407  GTAC                 GT^AC
              825  GTAC                
Asp700I       123  GAAGTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              330  GAACAAGTTC          
BsaHI         733  GGCGCC               G[GA]^CG[CT]C
              960  GACGCC              
BseRI         307  GAGGAG               GAGGAG(10/8)
              514  GAGGAG              
BsmFI         423  GTCCC                GGGAC(10/14)
              915  GTCCC               
BstAPI        182  GCACGAAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1083  GCACCCCATGC         
DsaI          763  CCGCGG               C^C[GA][CT]GG
             1004  CCGCGG              
EagI          786  CGGCCG               C^GGCCG
              920  CGGCCG              
EclXI         786  CGGCCG               C^GGCCG
              920  CGGCCG              
Eco52I        786  CGGCCG               C^GGCCG
              920  CGGCCG              
EcoT14I       729  CCTAGG               C^C[AT][AT]GG
              846  CCAAGG              
Hin1I         733  GGCGCC               G[GA]^CG[CT]C
              960  GACGCC              
KspI          763  CCGCGG               CCGC^GG
             1004  CCGCGG              
MaeII          56  ACGT                 A^CGT
              317  ACGT                
MboII         752  TCTTC                GAAGA(8/7)
              884  TCTTC               
NaeI          193  GCCGGC               GCC^GGC
              991  GCCGGC              
NgoMIV        193  GCCGGC               G^CCGGC
              991  GCCGGC              
NspI          659  ACATGC               [GA]CATG^[CT]
              770  GCATGC              
RsaI          407  GTAC                 GT^AC
              825  GTAC                
SacII         763  CCGCGG               CCGC^GG
             1004  CCGCGG              
SfaNI         209  GCATC                GCATC(5/9)
             1170  GCATC               
SfcI          445  CTGCAG               C^T[GA][CT]AG
             1152  CTACAG              
SmlI          373  CTCAAG               C^T[CT][GA]AG
              583  CTCAAG              
StyI          729  CCTAGG               C^C[AT][AT]GG
              846  CCAAGG              
XmnI          123  GAAGTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              330  GAACAAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AccII       AciI        AluI        AspHI       AvaI        
AvaII       BanI        BanII       BbvI        BcnI        
BmyI        BpmI        BsaJI       BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsoBI       
Bsp1286I    BsrBI       BsrFI       BssKI       BstF5I      
BstNI       BstUI       BstXI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        EaeI        EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NlaIII      NlaIV       PleI        PvuII       
SacI        Sau96I      ScrFI       TaqI        TseI        
TspRI       TthHB8I     
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