Restriction map for pF1KE0867
##################################
# NO CUT ENZYMES (  156 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AciI        
AclI        AflII       AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BanII       BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BglI        BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BsaAI       BsaI        
BseAI       BseRI       BsiEI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DraII       DraIII      DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HgaI        HincII      HindII      HindIII     HinfI       
HpaI        KpnI        KspI        MaeII       MluNI       
MroI        MscI        MspA1I      NaeI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RsrII       SacI        SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        TfiI        Tth111I     
TthHB8I     Van91I      XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccII         580  CGCG                 CG^CG
AcsI          922  AAATTC               [GA]^AATT[CT]
AcyI          701  GGCGCC               G[GA]^CG[CT]C
AfaI          177  GTAC                 GT^AC
AflIII        579  ACGCGT               A^C[GA][CT]GT
AlwNI          70  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          922  AAATTC               [GA]^AATT[CT]
BamHI         683  GGATCC               G^GATCC
BbeI          701  GGCGCC               GGCGC^C
BcnI          816  CCGGG                CC^[GC]GG
BfaI          205  CTAG                 C^TAG
BglII         521  AGATCT               A^GATCT
BsaBI         642  GATCTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         701  GGCGCC               G[GA]^CG[CT]C
BsaWI         686  TCCGGT               [AT]^CCGG[AT]
BsmFI         783  GTCCC                GGGAC(10/14)
BspHI         407  TCATGA               T^CATGA
BspMI         721  ACCTGC               ACCTGC(4/8)
BstUI         580  CGCG                 CG^CG
DraI          855  TTTAAA               TTT^AAA
DrdI           58  GACCCTGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          726  CTCTTC               CTCTTC(1/4)
HaeII         701  GGCGCC               [GA]GCGC^[CT]
Hin1I         701  GGCGCC               G[GA]^CG[CT]C
KasI          701  GGCGCC               G^GCGCC
Ksp632I       726  CTCTTC               CTCTTC(1/4)
MaeI          205  CTAG                 C^TAG
MamI          642  GATCTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          131  CAATTG               C^AATTG
MluI          579  ACGCGT               A^CGCGT
MunI          131  CAATTG               C^AATTG
MvnI          580  CGCG                 CG^CG
NarI          701  GGCGCC               GG^CGCC
NciI          816  CCGGG                CC^[GC]GG
NspI          542  ACATGC               [GA]CATG^[CT]
RcaI          407  TCATGA               T^CATGA
RsaI          177  GTAC                 GT^AC
SapI          725  GCTCTTC              GCTCTTC(1/4)
SfoI          701  GGCGCC               GGC^GCC
TspRI         470  CACTG                CA[GC]TG[ACGT][ACGT]^
XbaI          204  TCTAGA               T^CTAGA
XcmI          227  CCATCACCCCATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AlwI          684  GATCC                GGATC(4/5)
              683  GGATC               
AocI           61  CCTGAGG              CC^T[ACGT]AGG
              903  CCTAAGG             
BanI          295  GGCACC               G^G[CT][GA]CC
              701  GGCGCC              
BbvI          432  GCTGC                GCAGC(8/12)
              761  GCTGC               
BsgI          762  CTGCAC               GTGCAG(16/14)
              569  GTGCAG              
BsrI          366  CCAGT                ACTGG(1/-1)
              788  CCAGT               
BstF5I        627  CATCC                GGATG(2/0)
              289  GGATG               
BstNI         186  CCTGG                CC^[AT]GG
              561  CCTGG               
BstYI         521  AGATCT               [GA]^GATC[CT]
              683  GGATCC              
Bsu36I         61  CCTGAGG              CC^T[ACGT]AGG
              903  CCTAAGG             
CfoI          558  GCGC                 GCG^C
              702  GCGC                
Eco81I         61  CCTGAGG              CC^T[ACGT]AGG
              903  CCTAAGG             
EcoRII        186  CCTGG                ^CC[AT]GG
              561  CCTGG               
EcoT14I       242  CCTTGG               C^C[AT][AT]GG
              446  CCTTGG              
Fnu4HI        432  GCTGC                GC^[ACGT]GC
              761  GCTGC               
FokI          627  CATCC                GGATG(9/13)
              289  GGATG               
HapII         687  CCGG                 C^CGG
              816  CCGG                
HhaI          558  GCGC                 GCG^C
              702  GCGC                
HinP1I        558  GCGC                 G^CGC
              702  GCGC                
HpaII         687  CCGG                 C^CGG
              816  CCGG                
ItaI          432  GCTGC                GC^[ACGT]GC
              761  GCTGC               
MflI          521  AGATCT               [GA]^GATC[CT]
              683  GGATCC              
MseI          487  TTAA                 T^TAA
              856  TTAA                
MspI          687  CCGG                 C^CGG
              816  CCGG                
MvaI          186  CCTGG                CC^[AT]GG
              561  CCTGG               
PstI          548  CTGCAG               CTGCA^G
              751  CTGCAG              
StyI          242  CCTTGG               C^C[AT][AT]GG
              446  CCTTGG              
Tru9I         487  TTAA                 T^TAA
              856  TTAA                
TseI          432  GCTGC                G^C[AT]GC
              761  GCTGC               
Tsp509I       132  AATT                 ^AATT
              923  AATT                
XhoII         521  AGATCT               [GA]^GATC[CT]
              683  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AluI        AvaII       BbsI        BpuAI       BsaJI       
BsiYI       BslI        BssKI       Cac8I       Cfr13I      
DdeI        DpnII       HaeIII      HphI        MaeIII      
MboI        MboII       MnlI        MslI        MwoI        
NdeII       NlaIII      NlaIV       Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        Tsp45I      
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