Restriction map for pF1KE0839
##################################
# NO CUT ENZYMES (  167 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BcnI        BfrI        BglII       BlnI        BlpI        
BmyI        Bpu1102I    BpuAI       BsaAI       BsaHI       
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco52I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FseI        FspI        HapII       HgaI        Hin1I       
HpaII       KasI        KpnI        KspI        MaeII       
MfeI        MflI        MluI        MroI        MslI        
MspA1I      MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tth111I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AlwNI         162  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          772  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       760  AGCGCT               AGC^GCT
AvaII         407  GGTCC                G^G[AT]CC
BalI          152  TGGCCA               TGG^CCA
BbvI          207  GCTGC                GCAGC(8/12)
BglI          688  GCCATAAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          159  CTCCAG               CTGGAG(16/14)
BsaBI         129  GATTGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          659  GAGACC               GGTCTC(1/5)
BsaJI         552  CCTGGG               C^C[ACGT][ACGT]GG
BspHI         480  TCATGA               T^CATGA
BstF5I        138  CATCC                GGATG(2/0)
Bsu36I        772  CCTCAGG              CC^T[ACGT]AGG
CfoI          761  GCGC                 GCG^C
DraII         406  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EaeI          152  TGGCCA               [CT]^GGCC[GA]
EarI          113  GAAGAG               CTCTTC(1/4)
Eco47III      760  AGCGCT               AGC^GCT
Eco81I        772  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      406  GGGTCCT              [GA]G^G[ACGT]CC[CT]
Fnu4HI        207  GCTGC                GC^[ACGT]GC
FokI          138  CATCC                GGATG(9/13)
HaeII         760  AGCGCT               [GA]GCGC^[CT]
HhaI          761  GCGC                 GCG^C
HinP1I        761  GCGC                 G^CGC
HindIII       872  AAGCTT               A^AGCTT
ItaI          207  GCTGC                GC^[ACGT]GC
Ksp632I       113  GAAGAG               CTCTTC(1/4)
MamI          129  GATTGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         152  TGGCCA               TGG^CCA
MscI          152  TGGCCA               TGG^CCA
NdeI          224  CATATG               CA^TATG
NspI          642  GCATGC               [GA]CATG^[CT]
PpuMI         406  GGGTCCT              [GA]G^G[AT]CC[CT]
RcaI          480  TCATGA               T^CATGA
SfcI          647  CTACAG               C^T[GA][CT]AG
SmlI          325  CTCAAG               C^T[CT][GA]AG
SpeI           84  ACTAGT               A^CTAGT
SphI          642  GCATGC               GCATG^C
TaqI          636  TCGA                 T^CGA
TseI          207  GCTGC                G^C[AT]GC
Tsp45I        800  GTGAC                ^GT[GC]AC
TspRI         698  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       636  TCGA                 T^CGA
XcmI          349  CCATTTTTCTTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AcsI          502  AAATTC               [GA]^AATT[CT]
              518  AAATTC              
ApoI          502  AAATTC               [GA]^AATT[CT]
              518  AAATTC              
Asp700I       871  GAAGCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              900  GAAGTGTTTC          
BclI          143  TGATCA               T^GATCA
              701  TGATCA              
BseRI         715  CTCCTC               GAGGAG(10/8)
              718  CTCCTC              
BsmAI         659  GAGAC                GTCTC(1/5)
              287  GTCTC               
FbaI          143  TGATCA               T^GATCA
              701  TGATCA              
HincII         88  GTTAAC               GT[CT]^[GA]AC
              540  GTTAAC              
HindII         88  GTTAAC               GT[CT]^[GA]AC
              540  GTTAAC              
HpaI           88  GTTAAC               GTT^AAC
              540  GTTAAC              
MwoI           10  GCAATAGAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              688  GCCATAAAGGC         
NlaIV         406  GGGTCC               GG[ACGT]^[ACGT]CC
              564  GGTTCC              
XmnI          871  GAAGCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              900  GAAGTGTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AfaI        AluI        BfaI        BsiYI       BslI        
BsrI        BssKI       BstNI       Cac8I       Cfr13I      
DdeI        DpnII       EcoRII      HaeIII      HinfI       
HphI        MaeI        MaeIII      MboI        MboII       
MnlI        MseI        MvaI        NdeII       NlaIII      
RsaI        Sau3AI      Sau96I      ScrFI       TfiI        
Tru9I       Tsp509I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com