Restriction map for pF1KE0839
 (Circular) (Six-base) MAPSORT of: pF1KE0839.seq  Check: 4647  from: 1  to: 4049



pF1KE0839  4049 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AccI GT'mk_AC

Cuts at:   1087    1087 
   Size:       4049
 
Acc65I G'GTAC_C

Cuts at:   1065    1065 
   Size:       4049
 
AclI AA'CG_TT

Cuts at:   3889    3889 
   Size:       4049
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    493    1009    1874    2306    3053    3321     493 
   Size:        516     865     432     747     268    1221

  Fragments arranged by size: 

                1221     865     747     516     432     268
 
AfeI AGC'GCT

Cuts at:    870     870 
   Size:       4049
 
AflIII A'CryG_T

Cuts at:   1401    2505    3482    1401 
   Size:       1104     977    1968

  Fragments arranged by size: 

                1968    1104     977
 
AgeI A'CCGG_T

Cuts at:   2437    2437 
   Size:       4049
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    361     361 
   Size:       4049
 
AlwNI CAG_nnn'CTG

Cuts at:    275    1354    2921     275 
   Size:       1079    1567    1403

  Fragments arranged by size: 

                1567    1403    1079
 
ApaLI G'TGCA_C

Cuts at:   2819    2819 
   Size:       4049
 
ApoI r'AATT_y

Cuts at:    610     626    1053    3402    3658     610 
   Size:         16     427    2349     256    1001

  Fragments arranged by size: 

                2349    1001     427     256      16
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4049
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4049
 
AvaI C'yCGr_G

Cuts at:   1069    1069 
   Size:       4049
 
BamHI G'GATC_C

Cuts at:   1074    1074 
   Size:       4049
 
BanI G'GyrC_C

Cuts at:   1065    1479    1727    1762    1065 
   Size:        414     248      35    3352

  Fragments arranged by size: 

                3352     414     248      35
 
BanII G_rGCy'C

Cuts at:   1063    2093    1063 
   Size:       1030    3019
 
BbeI G_GCGC'C

Cuts at:   1483    1731    1483 
   Size:        248    3801
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    4015
 
BciVI GTATCCnnnnn_n'

Cuts at:   1947    2708    4049    1947 
   Size:        761    1341    1947

  Fragments arranged by size: 

                1947    1341     761
 
BclI T'GATC_A

Cuts at:    251     809    1569     251 
   Size:        558     760    2731

  Fragments arranged by size: 

                2731     760     558
 
BglI GCCn_nnn'nGGC

Cuts at:    802     802 
   Size:       4049
 
BglII A'GATC_T

Cuts at:   1564    3601    1564 
   Size:       2037    2012
 
BlpI GC'TnA_GC

Cuts at:   1161    1161 
   Size:       4049
 
Bme1580I G_kGCm'C

Cuts at:   1674    1767    2823    1674 
   Size:         93    1056    2900

  Fragments arranged by size: 

                2900    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    767     838    1261    1342    1677    3821     767 
   Size:         71     423      81     335    2144     995

  Fragments arranged by size: 

                2144     995     423     335      81      71
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2416    2416 
   Size:       4049
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    252     252 
   Size:       4049
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    418    1225    2104    2596    2894     418 
   Size:        807     879     492     298    1573

  Fragments arranged by size: 

                1573     879     807     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    761    3753     761 
   Size:       2992    1057
 
BsaAI yAC'GTr

Cuts at:   1402    1402 
   Size:       4049
 
BsaBI GATnn'nnATC

Cuts at:      6     241    1563    3600       6 
   Size:        235    1322    2037     455

  Fragments arranged by size: 

                2037    1322     455     235
 
BsaHI Gr'CG_yC

Cuts at:   1480    1728    2430    3926    1480 
   Size:        248     702    1496    1603

  Fragments arranged by size: 

                1603    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1238    1759    2437    2711    2858    3269    1238 
   Size:        521     678     274     147     411    2018

  Fragments arranged by size: 

                2018     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    814     817     814 
   Size:          3    4046
 
BseYI C'CCAG_C

Cuts at:   1474    2232    2809    3305    1474 
   Size:        758     577     496    2218

  Fragments arranged by size: 

                2218     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1591    1637    2845     104 
   Size:       1487      46    1208    1308

  Fragments arranged by size: 

                1487    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1063    1841    2031    2823    1063 
   Size:        778     190     792    2289

  Fragments arranged by size: 

                2289     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:   1063    1674    1767    1841    2031    2093    2823    1063 
   Size:        611      93      74     190      62     730    2289

  Fragments arranged by size: 

                2289     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   3269    3269 
   Size:       4049
 
BspHI T'CATG_A

Cuts at:    588    3225     588 
   Size:       2637    1412
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1099    1313    1615    1996    1099 
   Size:        214     302     381    3152

  Fragments arranged by size: 

                3152     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2341    2395    3879    4044      50 
   Size:       2291      54    1484     165      55

  Fragments arranged by size: 

                2291    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1961    1961 
   Size:       4049
 
BsrFI r'CCGG_y

Cuts at:   2047    2228    2437    2047 
   Size:        181     209    3659

  Fragments arranged by size: 

                3659     209     181
 
BssSI C'ACGA_G

Cuts at:   2320    2678    2320 
   Size:        358    3691
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1426    1426 
   Size:       4049
 
BstBI TT'CG_AA

Cuts at:   2410    2410 
   Size:       4049
 
BstYI r'GATC_y

Cuts at:      1    1074    1541    1564    1899    2145    3157    3243    3255 
   Size:       1073     467      23     335     246    1012      86      12

Cuts at:   3255    3601       1 
   Size:        346     449

  Fragments arranged by size: 

                1073    1012     467     449     346     335     246      86
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:    881     881 
   Size:       4049
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    682    1999    2180    2343     682 
   Size:       1317     181     163    2388

  Fragments arranged by size: 

                2388    1317     181     163
 
BtsI GCAGTG_nn'

Cuts at:    812    1292     812 
   Size:        480    3569
 
DraI TTT'AAA

Cuts at:   1048    1048 
   Size:       4049
 
DrdI GACnn_nn'nnGTC

Cuts at:   1755    2613    1755 
   Size:        858    3191
 
EaeI y'GGCC_r

Cuts at:    260    1634    1808    2199    2226    3571     260 
   Size:       1374     174     391      27    1345     738

  Fragments arranged by size: 

                1374    1345     738     391     174      27
 
EagI C'GGCC_G

Cuts at:   1634    1634 
   Size:       4049
 
EarI CTCTTCn'nnn_

Cuts at:    216    2072    2282     216 
   Size:       1856     210    1983

  Fragments arranged by size: 

                1983    1856     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2257    2567    2713    3859    2257 
   Size:        310     146    1146    2447

  Fragments arranged by size: 

                2447    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1061    1061 
   Size:       4049
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    252     493    1009    1874    2306    3053    3321     252 
   Size:        241     516     865     432     747     268     980

  Fragments arranged by size: 

                 980     865     747     516     432     268     241
 
EcoO109I rG'GnC_Cy

Cuts at:    515    1188     515 
   Size:        673    3376
 
EcoRI G'AATT_C

Cuts at:   1053    1053 
   Size:       4049
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1049    2068    1049 
   Size:       1019    3030
 
FspI TGC'GCA

Cuts at:   1830    1830 
   Size:       4049
 
HaeII r_GCGC'y

Cuts at:    872    1483    1731    2753    3732     872 
   Size:        611     248    1022     979    1189

  Fragments arranged by size: 

                1189    1022     979     611     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1891    1921    1923    1953    1891 
   Size:         30       2      30    3987

  Fragments arranged by size: 

                3987      30      30       2
 
HincII GTy'rAC

Cuts at:    198     650    1088     198 
   Size:        452     438    3159

  Fragments arranged by size: 

                3159     452     438
 
HindIII A'AGCT_T

Cuts at:    980     980 
   Size:       4049
 
HpaI GTT'AAC

Cuts at:    198     650     198 
   Size:        452    3597
 
KasI G'GCGC_C

Cuts at:   1479    1727    1479 
   Size:        248    3801
 
KpnI G_GTAC'C

Cuts at:   1069    1069 
   Size:       4049
 
MluI A'CGCG_T

Cuts at:   3482    3482 
   Size:       4049
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2720    2904    2720 
   Size:        184    3865
 
MscI TGG'CCA

Cuts at:    262    1810     262 
   Size:       1548    2501
 
MslI CAynn'nnrTG

Cuts at:   2038    2165    2038 
   Size:        127    3922
 
MspA1I CmG'CkG

Cuts at:   1160    1474    1834    2847    3092    1160 
   Size:        314     360    1013     245    2117

  Fragments arranged by size: 

                2117    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2230    2230 
   Size:       4049
 
NarI GG'CG_CC

Cuts at:   1480    1728    1480 
   Size:        248    3801
 
NdeI CA'TA_TG

Cuts at:    333     333 
   Size:       4049
 
NgoMIV G'CCGG_C

Cuts at:   2228    2228 
   Size:       4049
 
NspI r_CATG'y

Cuts at:    754    1102    1602    2509     754 
   Size:        348     500     907    2294

  Fragments arranged by size: 

                2294     907     500     348
 
PciI A'CATG_T

Cuts at:   2505    2505 
   Size:       4049
 
PmeI GTTT'AAAC

Cuts at:   1048    1048 
   Size:       4049
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3188    3220    3188 
   Size:         32    4017
 
PpuMI rG'GwC_Cy

Cuts at:    515     515 
   Size:       4049
 
PstI C_TGCA'G

Cuts at:   1096    1781    1096 
   Size:        685    3364
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4049
 
PvuII CAG'CTG

Cuts at:   1474    1834    1474 
   Size:        360    3689
 
RsrII CG'GwC_CG

Cuts at:   2244    2244 
   Size:       4049
 
SacI G_AGCT'C

Cuts at:   1063    1063 
   Size:       4049
 
SalI G'TCGA_C

Cuts at:   1086    1086 
   Size:       4049
 
SapI GCTCTTCn'nnn_

Cuts at:   2072    2282    2072 
   Size:        210    3839
 
SbfI CC_TGCA'GG

Cuts at:   1096    1096 
   Size:       4049
 
ScaI AGT'ACT

Cuts at:   3566    3566 
   Size:       4049
 
SfcI C'TryA_G

Cuts at:     33     755    1092    1777    2770    2961      33 
   Size:        722     337     685     993     191    1121

  Fragments arranged by size: 

                1121     993     722     685     337     191
 
SfoI GGC'GCC

Cuts at:   1481    1729    1481 
   Size:        248    3801
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4049
 
SmaI CCC'GGG

Cuts at:   1071    1071 
   Size:       4049
 
SmlI C'TyrA_G

Cuts at:    433    1204    2119    2611    2873     433 
   Size:        771     915     492     262    1609

  Fragments arranged by size: 

                1609     915     771     492     262
 
SpeI A'CTAG_T

Cuts at:     67     192      67 
   Size:        125    3924
 
SphI G_CATG'C

Cuts at:    754    1102    1602     754 
   Size:        348     500    3201

  Fragments arranged by size: 

                3201     500     348
 
StyI C'CwwG_G

Cuts at:   1183    1537    1183 
   Size:        354    3695
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    470    1632    1765    2224    2433     470 
   Size:       1162     133     459     209    2086

  Fragments arranged by size: 

                2086    1162     459     209     133
 
TatI w'GTAC_w

Cuts at:    189     775     836    3564     189 
   Size:        586      61    2728     674

  Fragments arranged by size: 

                2728     674     586      61
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    878     910    3566    3598     878 
   Size:         32    2656      32    1329

  Fragments arranged by size: 

                2656    1329      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1846    1846 
   Size:       4049
 
XbaI T'CTAG_A

Cuts at:   1080    1080 
   Size:       4049
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    464    1544     464 
   Size:       1080    2969
 
XmaI C'CCGG_G

Cuts at:   1069    1069 
   Size:       4049
 
XmnI GAAnn'nnTTC

Cuts at:    983    1012     983 
   Size:         29    4020
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI     AlfI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstYI   Bsu36I    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NspI     PciI     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AleI     AloI     ApaI     AscI
    AvrII     BaeI     BbsI    BbvCI    BfrBI    BmgBI     BmtI   Bpu10I
    BsaXI     BsgI    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII
    BstXI  BstZ17I     BtgI     ClaI    CspCI   DraIII    EcoNI    EcoRV
     FseI    FspAI     MfeI     NcoI     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SspI     StuI     SwaI     XhoI     ZraI
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