Restriction map for
pF1KE0836
(Circular) (Six-base) MAPSORT of: pF1KE0836.seq Check: 4236 from: 1 to: 4067
pF1KE0836 4067 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 1105 1105
Size: 4067
Acc65I G'GTAC_C
Cuts at: 1083 1083
Size: 4067
AclI AA'CG_TT
Cuts at: 3907 3907
Size: 4067
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 528 984 1048 1892 2324 3071 3339 528
Size: 456 64 844 432 747 268 1256
Fragments arranged by size:
1256 844 747 456 432 268 64
AflIII A'CryG_T
Cuts at: 1419 2523 3500 1419
Size: 1104 977 1986
Fragments arranged by size:
1986 1104 977
AgeI A'CCGG_T
Cuts at: 2455 2455
Size: 4067
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 766 766
Size: 4067
AlwNI CAG_nnn'CTG
Cuts at: 1007 1372 2939 1007
Size: 365 1567 2135
Fragments arranged by size:
2135 1567 365
ApaLI G'TGCA_C
Cuts at: 2837 2837
Size: 4067
ApoI r'AATT_y
Cuts at: 679 937 1071 3420 3676 679
Size: 258 134 2349 256 1070
Fragments arranged by size:
2349 1070 258 256 134
AseI AT'TA_AT
Cuts at: 20 20
Size: 4067
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4067
AvaI C'yCGr_G
Cuts at: 1087 1087
Size: 4067
AvrII C'CTAG_G
Cuts at: 291 291
Size: 4067
BamHI G'GATC_C
Cuts at: 1092 1092
Size: 4067
BanI G'GyrC_C
Cuts at: 1083 1497 1745 1780 1083
Size: 414 248 35 3370
Fragments arranged by size:
3370 414 248 35
BanII G_rGCy'C
Cuts at: 560 818 1081 2111 560
Size: 258 263 1030 2516
Fragments arranged by size:
2516 1030 263 258
BbeI G_GCGC'C
Cuts at: 1501 1749 1501
Size: 248 3819
BbsI GAAGACnn'nnnn_
Cuts at: 447 486 777 447
Size: 39 291 3737
Fragments arranged by size:
3737 291 39
BciVI GTATCCnnnnn_n'
Cuts at: 1965 2726 4067 1965
Size: 761 1341 1965
Fragments arranged by size:
1965 1341 761
BclI T'GATC_A
Cuts at: 1587 1587
Size: 4067
BglII A'GATC_T
Cuts at: 1582 3619 1582
Size: 2037 2030
BlpI GC'TnA_GC
Cuts at: 1179 1179
Size: 4067
Bme1580I G_kGCm'C
Cuts at: 1692 1785 2841 1692
Size: 93 1056 2918
Fragments arranged by size:
2918 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 812 1279 1360 1695 3839 812
Size: 467 81 335 2144 1040
Fragments arranged by size:
2144 1040 467 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2434 2434
Size: 4067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 546 546
Size: 4067
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1029 1243 2122 2614 2912 1029
Size: 214 879 492 298 2184
Fragments arranged by size:
2184 879 492 298 214
BsaI GGTCTCn'nnnn_
Cuts at: 110 3771 110
Size: 3661 406
BsaAI yAC'GTr
Cuts at: 1420 1420
Size: 4067
BsaBI GATnn'nnATC
Cuts at: 6 1581 3618 6
Size: 1575 2037 455
Fragments arranged by size:
2037 1575 455
BsaHI Gr'CG_yC
Cuts at: 1498 1746 2448 3944 1498
Size: 248 702 1496 1621
Fragments arranged by size:
1621 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1256 1777 2455 2729 2876 3287 1256
Size: 521 678 274 147 411 2036
Fragments arranged by size:
2036 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 657 687 657
Size: 30 4037
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 844 844
Size: 4067
BseYI C'CCAG_C
Cuts at: 1492 2250 2827 3323 1492
Size: 758 577 496 2236
Fragments arranged by size:
2236 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 992 844
Size: 148 3919
BsiEI CG_ry'CG
Cuts at: 104 1609 1655 2863 104
Size: 1505 46 1208 1308
Fragments arranged by size:
1505 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1081 1859 2049 2841 1081
Size: 778 190 792 2307
Fragments arranged by size:
2307 792 778 190
BsmI GAATG_Cn'
Cuts at: 423 423
Size: 4067
Bsp1286I G_dGCh'C
Cuts at: 560 818 1081 1692 1785 1859 2049 2111 2841
Size: 258 263 611 93 74 190 62 730
Cuts at: 2841 560
Size: 1786
Fragments arranged by size:
1786 730 611 263 258 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3287 3287
Size: 4067
BspHI T'CATG_A
Cuts at: 3243 3243
Size: 4067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 901 1117 1331 1633 2014 901
Size: 216 214 302 381 2954
Fragments arranged by size:
2954 381 302 216 214
BsrBI CCG'CTC
Cuts at: 50 2359 2413 3897 4062 50
Size: 2309 54 1484 165 55
Fragments arranged by size:
2309 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 216 1979 216
Size: 1763 2304
BsrFI r'CCGG_y
Cuts at: 2065 2246 2455 2065
Size: 181 209 3677
Fragments arranged by size:
3677 209 181
BssSI C'ACGA_G
Cuts at: 2338 2696 2338
Size: 358 3709
BstAPI GCAn_nnn'nTGC
Cuts at: 1444 1444
Size: 4067
BstBI TT'CG_AA
Cuts at: 2428 2428
Size: 4067
BstEII G'GTnAC_C
Cuts at: 168 168
Size: 4067
BstXI CCAn_nnnn'nTGG
Cuts at: 165 165
Size: 4067
BstYI r'GATC_y
Cuts at: 1 133 1092 1559 1582 1917 2163 3175 3261
Size: 132 959 467 23 335 246 1012 86
Cuts at: 3261 3273 3619 1
Size: 12 346 449
Fragments arranged by size:
1012 959 467 449 346 335 246 132
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 801 801
Size: 4067
BtgI C'CryG_G
Cuts at: 532 532
Size: 4067
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2017 2198 2361 2017
Size: 181 163 3723
Fragments arranged by size:
3723 181 163
BtsI GCAGTG_nn'
Cuts at: 221 722 1310 221
Size: 501 588 2978
Fragments arranged by size:
2978 588 501
DraI TTT'AAA
Cuts at: 936 1066 936
Size: 130 3937
DrdI GACnn_nn'nnGTC
Cuts at: 1773 2631 1773
Size: 858 3209
EaeI y'GGCC_r
Cuts at: 1652 1826 2217 2244 3589 1652
Size: 174 391 27 1345 2130
Fragments arranged by size:
2130 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1652 1652
Size: 4067
EarI CTCTTCn'nnn_
Cuts at: 547 694 877 1003 1024 2090 2300 547
Size: 147 183 126 21 1066 210 2314
Fragments arranged by size:
2314 1066 210 183 147 126 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2275 2585 2731 3877 2275
Size: 310 146 1146 2465
Fragments arranged by size:
2465 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1079 1079
Size: 4067
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 528 546 984 1048 1892 2324 3071 3339 528
Size: 18 438 64 844 432 747 268 1256
Fragments arranged by size:
1256 844 747 438 432 268 64 18
EcoO109I rG'GnC_Cy
Cuts at: 296 1206 296
Size: 910 3157
EcoRI G'AATT_C
Cuts at: 1071 1071
Size: 4067
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2086 2086
Size: 4067
FspI TGC'GCA
Cuts at: 1848 1848
Size: 4067
HaeII r_GCGC'y
Cuts at: 1501 1749 2771 3750 1501
Size: 248 1022 979 1818
Fragments arranged by size:
1818 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1909 1939 1941 1971 1909
Size: 30 2 30 4005
Fragments arranged by size:
4005 30 30 2
HincII GTy'rAC
Cuts at: 1106 1106
Size: 4067
KasI G'GCGC_C
Cuts at: 1497 1745 1497
Size: 248 3819
KpnI G_GTAC'C
Cuts at: 1087 1087
Size: 4067
MluI A'CGCG_T
Cuts at: 3500 3500
Size: 4067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2738 2922 2738
Size: 184 3883
MscI TGG'CCA
Cuts at: 1828 1828
Size: 4067
MslI CAynn'nnrTG
Cuts at: 742 2056 2183 742
Size: 1314 127 2626
Fragments arranged by size:
2626 1314 127
MspA1I CmG'CkG
Cuts at: 1178 1492 1852 2865 3110 1178
Size: 314 360 1013 245 2135
Fragments arranged by size:
2135 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2248 2248
Size: 4067
NarI GG'CG_CC
Cuts at: 1498 1746 1498
Size: 248 3819
NcoI C'CATG_G
Cuts at: 532 532
Size: 4067
NgoMIV G'CCGG_C
Cuts at: 2246 2246
Size: 4067
NspI r_CATG'y
Cuts at: 1120 1620 2527 1120
Size: 500 907 2660
Fragments arranged by size:
2660 907 500
PciI A'CATG_T
Cuts at: 2523 2523
Size: 4067
PflMI CCAn_nnn'nTGG
Cuts at: 431 431
Size: 4067
PmeI GTTT'AAAC
Cuts at: 1066 1066
Size: 4067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1023 1055 3206 3238 1023
Size: 32 2151 32 1852
Fragments arranged by size:
2151 1852 32 32
PstI C_TGCA'G
Cuts at: 317 389 912 1012 1114 1799 317
Size: 72 523 100 102 685 2585
Fragments arranged by size:
2585 685 523 102 100 72
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4067
PvuII CAG'CTG
Cuts at: 1492 1852 1492
Size: 360 3707
RsrII CG'GwC_CG
Cuts at: 2262 2262
Size: 4067
SacI G_AGCT'C
Cuts at: 1081 1081
Size: 4067
SalI G'TCGA_C
Cuts at: 1104 1104
Size: 4067
SapI GCTCTTCn'nnn_
Cuts at: 547 877 1003 2090 2300 547
Size: 330 126 1087 210 2314
Fragments arranged by size:
2314 1087 330 210 126
SbfI CC_TGCA'GG
Cuts at: 1114 1114
Size: 4067
ScaI AGT'ACT
Cuts at: 3584 3584
Size: 4067
SfcI C'TryA_G
Cuts at: 33 313 385 551 908 1008 1110 1795 2788
Size: 280 72 166 357 100 102 685 993
Cuts at: 2788 2979 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 357 280 191 166 102
100 72
SfoI GGC'GCC
Cuts at: 1499 1747 1499
Size: 248 3819
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4067
SmaI CCC'GGG
Cuts at: 1089 1089
Size: 4067
SmlI C'TyrA_G
Cuts at: 1044 1222 2137 2629 2891 1044
Size: 178 915 492 262 2220
Fragments arranged by size:
2220 915 492 262 178
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4067
SphI G_CATG'C
Cuts at: 1120 1620 1120
Size: 500 3567
StyI C'CwwG_G
Cuts at: 291 532 637 1201 1555 291
Size: 241 105 564 354 2803
Fragments arranged by size:
2803 564 354 241 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 234 499 512 933 1650 1783 2242 2451 234
Size: 265 13 421 717 133 459 209 1850
Fragments arranged by size:
1850 717 459 421 265 209 133 13
TatI w'GTAC_w
Cuts at: 381 3582 381
Size: 3201 866
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 125 157 657 689 3584 3616 125
Size: 32 500 32 2895 32 576
Fragments arranged by size:
2895 576 500 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1864 1864
Size: 4067
XbaI T'CTAG_A
Cuts at: 1098 1098
Size: 4067
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1562 1562
Size: 4067
XmaI C'CCGG_G
Cuts at: 1087 1087
Size: 4067
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsiWI BsmBI BsrGI BssHII BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI