Restriction map for pF1KE0734
 (Circular) (Six-base) MAPSORT of: pF1KE0734.seq  Check: 7217  from: 1  to: 4379



pF1KE0734  4379 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    388     388 
   Size:       4379
 
AccI GT'mk_AC

Cuts at:    627    1417     627 
   Size:        790    3589
 
Acc65I G'GTAC_C

Cuts at:   1395    1395 
   Size:       4379
 
AclI AA'CG_TT

Cuts at:   4219    4219 
   Size:       4379
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    222    2204    2636    3383    3651     222 
   Size:       1982     432     747     268     950

  Fragments arranged by size: 

                1982     950     747     432     268
 
AflIII A'CryG_T

Cuts at:   1731    2835    3812    1731 
   Size:       1104     977    2298

  Fragments arranged by size: 

                2298    1104     977
 
AgeI A'CCGG_T

Cuts at:   2767    2767 
   Size:       4379
 
AlwNI CAG_nnn'CTG

Cuts at:   1684    3251    1684 
   Size:       1567    2812
 
ApaLI G'TGCA_C

Cuts at:    173     571    3149     173 
   Size:        398    2578    1403

  Fragments arranged by size: 

                2578    1403     398
 
ApoI r'AATT_y

Cuts at:   1383    3732    3988    1383 
   Size:       2349     256    1774

  Fragments arranged by size: 

                2349    1774     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4379
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4379
 
AvaI C'yCGr_G

Cuts at:   1157    1399    1157 
   Size:        242    4137
 
AvrII C'CTAG_G

Cuts at:    816     816 
   Size:       4379
 
BamHI G'GATC_C

Cuts at:   1404    1404 
   Size:       4379
 
BanI G'GyrC_C

Cuts at:   1395    1809    2057    2092    1395 
   Size:        414     248      35    3682

  Fragments arranged by size: 

                3682     414     248      35
 
BanII G_rGCy'C

Cuts at:    281     467    1266    1393    2423     281 
   Size:        186     799     127    1030    2237

  Fragments arranged by size: 

                2237    1030     799     186     127
 
BbeI G_GCGC'C

Cuts at:   1813    2061    1813 
   Size:        248    4131
 
BbsI GAAGACnn'nnnn_

Cuts at:    529    1360     529 
   Size:        831    3548
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1186    1220    1186 
   Size:         34    4345
 
BciVI GTATCCnnnnn_n'

Cuts at:   2277    3038    4379    2277 
   Size:        761    1341    2277

  Fragments arranged by size: 

                2277    1341     761
 
BclI T'GATC_A

Cuts at:   1899    1899 
   Size:       4379
 
BglII A'GATC_T

Cuts at:   1894    3931    1894 
   Size:       2037    2342
 
BlpI GC'TnA_GC

Cuts at:   1361    1491    1361 
   Size:        130    4249
 
Bme1580I G_kGCm'C

Cuts at:    177     575    2004    2097    3153     177 
   Size:        398    1429      93    1056    1403

  Fragments arranged by size: 

                1429    1403    1056     398      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1591    1672    2007    4151    1591 
   Size:         81     335    2144    1819

  Fragments arranged by size: 

                2144    1819     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    868    2746     868 
   Size:       1878    2501
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    481    1097     481 
   Size:        616    3763
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    853    1555    2434    2926    3224     853 
   Size:        702     879     492     298    2008

  Fragments arranged by size: 

                2008     879     702     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    412    1092    4083     412 
   Size:        680    2991     708

  Fragments arranged by size: 

                2991     708     680
 
BsaAI yAC'GTr

Cuts at:    346     570    1131    1732     346 
   Size:        224     561     601    2993

  Fragments arranged by size: 

                2993     601     561     224
 
BsaBI GATnn'nnATC

Cuts at:      6     541    1893    3930       6 
   Size:        535    1352    2037     455

  Fragments arranged by size: 

                2037    1352     535     455
 
BsaHI Gr'CG_yC

Cuts at:   1810    2058    2760    4256    1810 
   Size:        248     702    1496    1933

  Fragments arranged by size: 

                1933    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1568    2089    2767    3041    3188    3599    1568 
   Size:        521     678     274     147     411    2348

  Fragments arranged by size: 

                2348     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    475     505     475 
   Size:         30    4349
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    189     973     189 
   Size:        784    3595
 
BseYI C'CCAG_C

Cuts at:   1804    2562    3139    3635    1804 
   Size:        758     577     496    2548

  Fragments arranged by size: 

                2548     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    416     416 
   Size:       4379
 
BsiEI CG_ry'CG

Cuts at:    104    1921    1967    3175     104 
   Size:       1817      46    1208    1308

  Fragments arranged by size: 

                1817    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    177     281     351     467     575    1393    2171    2361    3153 
   Size:        104      70     116     108     818     778     190     792

Cuts at:   3153     177 
   Size:       1403

  Fragments arranged by size: 

                1403     818     792     778     190     116     108     104
                  70
 
Bsp1286I G_dGCh'C

Cuts at:    177     281     351     467     575    1266    1393    2004    2097 
   Size:        104      70     116     108     691     127     611      93

Cuts at:   2097    2171    2361    2423    3153     177 
   Size:         74     190      62     730    1403

  Fragments arranged by size: 

                1403     730     691     611     190     127     116     108
                 104      93      74      70      62
 
BspEI T'CCGG_A

Cuts at:   3599    3599 
   Size:       4379
 
BspHI T'CATG_A

Cuts at:   3555    3555 
   Size:       4379
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    388    1429    1643    1945    2326     388 
   Size:       1041     214     302     381    2441

  Fragments arranged by size: 

                2441    1041     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2671    2725    4209    4374      50 
   Size:       2621      54    1484     165      55

  Fragments arranged by size: 

                2621    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2291    2291 
   Size:       4379
 
BsrFI r'CCGG_y

Cuts at:   2377    2558    2767    2377 
   Size:        181     209    3989

  Fragments arranged by size: 

                3989     209     181
 
BssSI C'ACGA_G

Cuts at:   2650    3008    2650 
   Size:        358    4021
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1756    1756 
   Size:       4379
 
BstBI TT'CG_AA

Cuts at:   1206    2740    1206 
   Size:       1534    2845
 
BstXI CCAn_nnnn'nTGG

Cuts at:    459     459 
   Size:       4379
 
BstYI r'GATC_y

Cuts at:      1    1404    1871    1894    2229    2475    3487    3573    3585 
   Size:       1403     467      23     335     246    1012      86      12

Cuts at:   3585    3931       1 
   Size:        346     449

  Fragments arranged by size: 

                1403    1012     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     158     452    1256     107 
   Size:         51     294     804    3230

  Fragments arranged by size: 

                3230     804     294      51
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2329    2510    2673    2329 
   Size:        181     163    4035

  Fragments arranged by size: 

                4035     181     163
 
BtsI GCAGTG_nn'

Cuts at:    177     399    1622     177 
   Size:        222    1223    2934

  Fragments arranged by size: 

                2934    1223     222
 
DraI TTT'AAA

Cuts at:   1378    1378 
   Size:       4379
 
DrdI GACnn_nn'nnGTC

Cuts at:   2085    2943    2085 
   Size:        858    3521
 
EaeI y'GGCC_r

Cuts at:    677    1183    1964    2138    2529    2556    3901     677 
   Size:        506     781     174     391      27    1345    1155

  Fragments arranged by size: 

                1345    1155     781     506     391     174      27
 
EagI C'GGCC_G

Cuts at:   1964    1964 
   Size:       4379
 
EarI CTCTTCn'nnn_

Cuts at:   1042    1208    2402    2612    1042 
   Size:        166    1194     210    2809

  Fragments arranged by size: 

                2809    1194     210     166
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2587    2897    3043    4189    2587 
   Size:        310     146    1146    2777

  Fragments arranged by size: 

                2777    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    279     465    1391     279 
   Size:        186     926    3267

  Fragments arranged by size: 

                3267     926     186
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    222     481    1097    2204    2636    3383    3651     222 
   Size:        259     616    1107     432     747     268     950

  Fragments arranged by size: 

                1107     950     747     616     432     268     259
 
EcoO109I rG'GnC_Cy

Cuts at:    229    1518     229 
   Size:       1289    3090
 
EcoRI G'AATT_C

Cuts at:   1383    1383 
   Size:       4379
 
EcoRV GAT'ATC

Cuts at:    895     895 
   Size:       4379
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2398    2398 
   Size:       4379
 
FspI TGC'GCA

Cuts at:   2160    2160 
   Size:       4379
 
HaeII r_GCGC'y

Cuts at:   1368    1813    2061    3083    4062    1368 
   Size:        445     248    1022     979    1685

  Fragments arranged by size: 

                1685    1022     979     445     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1171    1203    2221    2251    2253    2283    1171 
   Size:         32    1018      30       2      30    3267

  Fragments arranged by size: 

                3267    1018      32      30      30       2
 
HincII GTy'rAC

Cuts at:    628    1418     628 
   Size:        790    3589
 
KasI G'GCGC_C

Cuts at:   1809    2057    1809 
   Size:        248    4131
 
KpnI G_GTAC'C

Cuts at:   1399    1399 
   Size:       4379
 
MluI A'CGCG_T

Cuts at:   3812    3812 
   Size:       4379
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3050    3234    3050 
   Size:        184    4195
 
MscI TGG'CCA

Cuts at:    679    2140     679 
   Size:       1461    2918
 
MslI CAynn'nnrTG

Cuts at:   1271    2368    2495    1271 
   Size:       1097     127    3155

  Fragments arranged by size: 

                3155    1097     127
 
MspA1I CmG'CkG

Cuts at:    549    1490    1804    2164    3177    3422     549 
   Size:        941     314     360    1013     245    1506

  Fragments arranged by size: 

                1506    1013     941     360     314     245
 
NaeI GCC'GGC

Cuts at:   2560    2560 
   Size:       4379
 
NarI GG'CG_CC

Cuts at:   1810    2058    1810 
   Size:        248    4131
 
NcoI C'CATG_G

Cuts at:    107     158     452     107 
   Size:         51     294    4034

  Fragments arranged by size: 

                4034     294      51
 
NdeI CA'TA_TG

Cuts at:    824     824 
   Size:       4379
 
NgoMIV G'CCGG_C

Cuts at:   2558    2558 
   Size:       4379
 
NspI r_CATG'y

Cuts at:    652    1432    1932    2839     652 
   Size:        780     500     907    2192

  Fragments arranged by size: 

                2192     907     780     500
 
PciI A'CATG_T

Cuts at:   2835    2835 
   Size:       4379
 
PmeI GTTT'AAAC

Cuts at:   1378    1378 
   Size:       4379
 
PmlI CAC'GTG

Cuts at:    346     346 
   Size:       4379
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3518    3550    3518 
   Size:         32    4347
 
PpuMI rG'GwC_Cy

Cuts at:    229     229 
   Size:       4379
 
PsiI TTA'TAA

Cuts at:    380     380 
   Size:       4379
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    574     606    1109    1141     574 
   Size:         32     503      32    3812

  Fragments arranged by size: 

                3812     503      32      32
 
PstI C_TGCA'G

Cuts at:   1426    2111    1426 
   Size:        685    3694
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4379
 
PvuII CAG'CTG

Cuts at:    549    1804    2164     549 
   Size:       1255     360    2764

  Fragments arranged by size: 

                2764    1255     360
 
RsrII CG'GwC_CG

Cuts at:   2574    2574 
   Size:       4379
 
SacI G_AGCT'C

Cuts at:    281     467    1393     281 
   Size:        186     926    3267

  Fragments arranged by size: 

                3267     926     186
 
SalI G'TCGA_C

Cuts at:    626    1416     626 
   Size:        790    3589
 
SanDI GG'GwC_CC

Cuts at:    229     229 
   Size:       4379
 
SapI GCTCTTCn'nnn_

Cuts at:   2402    2612    2402 
   Size:        210    4169
 
SbfI CC_TGCA'GG

Cuts at:   1426    1426 
   Size:       4379
 
ScaI AGT'ACT

Cuts at:   3896    3896 
   Size:       4379
 
SfcI C'TryA_G

Cuts at:     33    1422    2107    3100    3291      33 
   Size:       1389     685     993     191    1121

  Fragments arranged by size: 

                1389    1121     993     685     191
 
SfoI GGC'GCC

Cuts at:   1811    2059    1811 
   Size:        248    4131
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4379
 
SmaI CCC'GGG

Cuts at:   1401    1401 
   Size:       4379
 
SmlI C'TyrA_G

Cuts at:    868    1157    1534    2449    2941    3203     868 
   Size:        289     377     915     492     262    2044

  Fragments arranged by size: 

                2044     915     492     377     289     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4379
 
SphI G_CATG'C

Cuts at:   1432    1932    1432 
   Size:        500    3879
 
StyI C'CwwG_G

Cuts at:    107     158     452     468     680     816    1513    1867     107 
   Size:         51     294      16     212     136     697     354    2619

  Fragments arranged by size: 

                2619     697     354     294     212     136      51      16
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    521    1962    2095    2554    2763     521 
   Size:       1441     133     459     209    2137

  Fragments arranged by size: 

                2137    1441     459     209     133
 
TatI w'GTAC_w

Cuts at:    368    3894     368 
   Size:       3526     853
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1306    1338    3896    3928    1306 
   Size:         32    2558      32    1757

  Fragments arranged by size: 

                2558    1757      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2176    2176 
   Size:       4379
 
XbaI T'CTAG_A

Cuts at:   1410    1410 
   Size:       4379
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1874    1874 
   Size:       4379
 
XhoI C'TCGA_G

Cuts at:   1157    1157 
   Size:       4379
 
XmaI C'CCGG_G

Cuts at:   1399    1399 
   Size:       4379
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NspI     PciI     PmeI     PmlI     PpiI
    PpuMI     PsiI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI     BaeI    BbvCI    BfrBI     BglI    BmgBI     BmtI   Bpu10I
    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII  BstZ17I   Bsu36I
     ClaI    CspCI   DraIII    EcoNI     FseI    FspAI  HindIII     HpaI
     MfeI     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI    PshAI   PspOMI    PspXI    SacII    SexAI     SfiI    SgrAI
    SnaBI     SrfI     SspI     StuI     SwaI     XmnI     ZraI
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