Restriction map for pF1KE0707
 (Linear) (Six-base) MAPSORT of: KIEE0707.seq  Check: 23  from: 1  to: 1278



KIEE0707.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     853    1278 
   Size:        853     425
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     282    1278 
   Size:        282     996
 
ApaI G_GGCC'C

Cuts at:      0     161     528     634     678     933    1278 
   Size:        161     367     106      44     255     345

  Fragments arranged by size: 

                 367     345     255     161     106      44
 
ApaLI G'TGCA_C

Cuts at:      0     490    1009    1278 
   Size:        490     519     269

  Fragments arranged by size: 

                 519     490     269
 
AvaI C'yCGr_G

Cuts at:      0    1117    1278 
   Size:       1117     161
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     133     166    1278 
   Size:        133      33    1112

  Fragments arranged by size: 

                1112     133      33
 
BanII G_rGCy'C

Cuts at:      0     161     353     528     634     678     820     933    1045 
   Size:        161     192     175     106      44     142     113     112

Cuts at:   1045    1207    1278 
   Size:        162      71

  Fragments arranged by size: 

                 192     175     162     161     142     113     112     106
                  71      44
 
BbvCI CC'TCA_GC

Cuts at:      0    1165    1278 
   Size:       1165     113
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     847     881    1278 
   Size:        847      34     397

  Fragments arranged by size: 

                 847     397      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     599    1278 
   Size:        599     679
 
Bme1580I G_kGCm'C

Cuts at:      0     101     161     494     528     623     634     678     933 
   Size:        101      60     333      34      95      11      44     255

Cuts at:    933    1013    1278 
   Size:         80     265

  Fragments arranged by size: 

                 333     265     255     101      95      80      60      44
                  34      11
 
BmgBI CAC'GTC

Cuts at:      0    1014    1278 
   Size:       1014     264
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     628    1278 
   Size:        628     650
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      56     367     484     838    1278 
   Size:         56     311     117     354     440

  Fragments arranged by size: 

                 440     354     311     117      56
 
Bpu10I CC'TnA_GC

Cuts at:      0     463    1165    1278 
   Size:        463     702     113

  Fragments arranged by size: 

                 702     463     113
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130    1278 
   Size:        130    1148
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      32    1087    1258    1278 
   Size:         32    1055     171      20

  Fragments arranged by size: 

                1055     171      32      20
 
BsaAI yAC'GTr

Cuts at:      0     618    1008    1278 
   Size:        618     390     270

  Fragments arranged by size: 

                 618     390     270
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1003    1033    1096    1126    1278 
   Size:       1003      30      63      30     152

  Fragments arranged by size: 

                1003     152      63      30      30
 
BseYI C'CCAG_C

Cuts at:      0     161     384     926    1217    1234    1278 
   Size:        161     223     542     291      17      44

  Fragments arranged by size: 

                 542     291     223     161      44      17
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     580     598     715     973    1278 
   Size:        580      18     117     258     305

  Fragments arranged by size: 

                 580     305     258     117      18
 
BsiEI CG_ry'CG

Cuts at:      0     867     948    1278 
   Size:        867      81     330

  Fragments arranged by size: 

                 867     330      81
 
BsiHKAI G_wGCw'C

Cuts at:      0     353     449     494     749    1013    1064    1278 
   Size:        353      96      45     255     264      51     214

  Fragments arranged by size: 

                 353     264     255     214      96      51      45
 
Bsp1286I G_dGCh'C

Cuts at:      0     101     161     353     449     494     528     623     634 
   Size:        101      60     192      96      45      34      95      11

Cuts at:    634     678     749     820     933    1013    1045    1064    1207 
   Size:         44      71      71     113      80      32      19     143

Cuts at:   1207    1278 
   Size:         71

  Fragments arranged by size: 

                 192     143     113     101      96      95      80      71
                  71      71      60      45      44      34      32      19
                  11
 
BspHI T'CATG_A

Cuts at:      0     737    1278 
   Size:        737     541
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     209     326     761     853    1278 
   Size:        209     117     435      92     425

  Fragments arranged by size: 

                 435     425     209     117      92
 
BsrDI GCAATG_nn'

Cuts at:      0      84     237    1278 
   Size:         84     153    1041

  Fragments arranged by size: 

                1041     153      84
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     513    1278 
   Size:        513     765
 
Bsu36I CC'TnA_GG

Cuts at:      0     110    1278 
   Size:        110    1168
 
BtgI C'CryG_G

Cuts at:      0      68     485     512     670     728    1278 
   Size:         68     417      27     158      58     550

  Fragments arranged by size: 

                 550     417     158      68      58      27
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1117    1278 
   Size:       1117     161
 
BtsI GCAGTG_nn'

Cuts at:      0      90     455    1278 
   Size:         90     365     823

  Fragments arranged by size: 

                 823     365      90
 
DraIII CAC_nnn'GTG

Cuts at:      0    1193    1278 
   Size:       1193      85
 
EaeI y'GGCC_r

Cuts at:      0      89     305     482     731    1049    1278 
   Size:         89     216     177     249     318     229

  Fragments arranged by size: 

                 318     249     229     216     177      89
 
EarI CTCTTCn'nnn_

Cuts at:      0     301     718    1278 
   Size:        301     417     560

  Fragments arranged by size: 

                 560     417     301
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     655    1278 
   Size:        655     623
 
EcoICRI GAG'CTC

Cuts at:      0     351    1278 
   Size:        351     927
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      56     282     367     484     838    1278 
   Size:         56     226      85     117     354     440

  Fragments arranged by size: 

                 440     354     226     117      85      56
 
EcoO109I rG'GnC_Cy

Cuts at:      0     157     158     630     675     930    1107    1121    1278 
   Size:        157       1     472      45     255     177      14     157

  Fragments arranged by size: 

                 472     255     177     157     157      45      14       1
            
 
FspI TGC'GCA

Cuts at:      0     274    1278 
   Size:        274    1004
 
FspAI rTGC'GCAy

Cuts at:      0     274    1278 
   Size:        274    1004
 
HaeII r_GCGC'y

Cuts at:      0     224    1278 
   Size:        224    1054
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1189    1221    1278 
   Size:       1189      32      57

  Fragments arranged by size: 

                1189      57      32
 
MfeI C'AATT_G

Cuts at:      0      18    1278 
   Size:         18    1260
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     293    1278 
   Size:        293     985
 
MscI TGG'CCA

Cuts at:      0     733    1278 
   Size:        733     545
 
MslI CAynn'nnrTG

Cuts at:      0     691     742    1278 
   Size:        691      51     536

  Fragments arranged by size: 

                 691     536      51
 
MspA1I CmG'CkG

Cuts at:      0     612     649    1217    1278 
   Size:        612      37     568      61

  Fragments arranged by size: 

                 612     568      61      37
 
NcoI C'CATG_G

Cuts at:      0      68     512     728    1278 
   Size:         68     444     216     550

  Fragments arranged by size: 

                 550     444     216      68
 
NspI r_CATG'y

Cuts at:      0     660    1278 
   Size:        660     618
 
PasI CC'CwG_GG

Cuts at:      0      49    1209    1278 
   Size:         49    1160      69

  Fragments arranged by size: 

                1160      69      49
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1230    1278 
   Size:       1230      48
 
PfoI T'CCnGG_A

Cuts at:      0    1100    1278 
   Size:       1100     178
 
PmlI CAC'GTG

Cuts at:      0     618    1008    1278 
   Size:        618     390     270

  Fragments arranged by size: 

                 618     390     270
 
PspOMI G'GGCC_C

Cuts at:      0     157     524     630     674     929    1278 
   Size:        157     367     106      44     255     349

  Fragments arranged by size: 

                 367     349     255     157     106      44
 
PstI C_TGCA'G

Cuts at:      0     825     850    1278 
   Size:        825      25     428

  Fragments arranged by size: 

                 825     428      25
 
PvuI CG_AT'CG

Cuts at:      0     867    1278 
   Size:        867     411
 
PvuII CAG'CTG

Cuts at:      0     612     649    1217    1278 
   Size:        612      37     568      61

  Fragments arranged by size: 

                 612     568      61      37
 
SacI G_AGCT'C

Cuts at:      0     353    1278 
   Size:        353     925
 
SapI GCTCTTCn'nnn_

Cuts at:      0     718    1278 
   Size:        718     560
 
SbfI CC_TGCA'GG

Cuts at:      0     850    1278 
   Size:        850     428
 
SfcI C'TryA_G

Cuts at:      0     821     846    1065    1278 
   Size:        821      25     219     213

  Fragments arranged by size: 

                 821     219     213      25
 
SmlI C'TyrA_G

Cuts at:      0     145    1278 
   Size:        145    1133
 
SphI G_CATG'C

Cuts at:      0     660    1278 
   Size:        660     618
 
StyI C'CwwG_G

Cuts at:      0      68     512     728     806    1278 
   Size:         68     444     216      78     472

  Fragments arranged by size: 

                 472     444     216      78      68
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     509    1278 
   Size:        509     769
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1003    1035    1096    1128    1278 
   Size:       1003      32      61      32     150

  Fragments arranged by size: 

                1003     150      61      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     949    1278 
   Size:        949     329
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1231    1278 
   Size:       1231      47
 

 Enzymes that do cut:

     AarI     AcuI     ApaI    ApaLI     AvaI     BaeI    BanII    BbvCI
     BcgI     BglI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspHI    BspMI    BsrDI    BstXI   Bsu36I     BtgI    BtgZI     BtsI
   DraIII     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I     FspI
    FspAI    HaeII    Hin4I     MfeI     MmeI     MscI     MslI   MspA1I
     NcoI     NspI     PasI    PflMI     PfoI     PmlI   PspOMI     PstI
     PvuI    PvuII     SacI     SapI     SbfI     SfcI     SmlI     SphI
     StyI    TaqII     TstI  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApoI     AscI     AseI
    AsiSI    AvrII    BamHI     BanI     BbeI     BbsI    BciVI     BclI
    BfrBI    BglII     BlpI     BmtI     BplI    BsaBI    BsaHI    BsaWI
    BseRI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I     ClaI
    CspCI     DraI     DrdI     EagI    EcoNI    EcoRI    EcoRV     FalI
     FseI   HincII  HindIII     HpaI     KasI     KpnI     MluI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PciI     PmeI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI
    RsrII    SacII     SalI    SanDI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI
     SwaI     TatI     XbaI     XhoI     XmaI     XmnI     ZraI
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