Restriction map for pF1KE0695
##################################
# NO CUT ENZYMES (  155 enzymes) #
##################################

AatII       AccI        AccII       AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        AspEI       
AspI        AvaI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BsaAI       
BsaBI       BsaHI       BsaI        BsgI        BsiWI       
BsmAI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CfoI        Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        Eam1105I    Eco47III    Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
FauI        FbaI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HpaI        HphI        KasI        KspI        
MaeII       MamI        MfeI        MflI        MluI        
MslI        MspA1I      MunI        MvnI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TfiI        Tth111I     
XcmI        XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

Acc65I         12  GGTACC               G^GTACC
AccIII         70  TCCGGA               T^CCGGA
AlwI          389  GGATC                GGATC(4/5)
AlwNI         643  CAGAAACTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       471  GAAGTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I        12  GGTACC               G^GTACC
AspHI          66  GAGCTC               G[AT]GC[AT]^C
BalI          780  TGGCCA               TGG^CCA
BcnI          144  CCGGG                CC^[GC]GG
BglI          226  GCCCAAAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI           63  GCTGAGC              GC^T[ACGT]AGC
BpmI          266  CTCCAG               CTGGAG(16/14)
Bpu1102I       63  GCTGAGC              GC^T[ACGT]AGC
BseAI          70  TCCGGA               T^CCGGA
BsiHKAI        66  GAGCTC               G[AT]GC[AT]^C
BsmFI         754  GTCCC                GGGAC(10/14)
BsmI          637  GCATTC               GAATGC(1/-1)
BspEI          70  TCCGGA               T^CCGGA
BstAPI        208  GCAAGGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII          63  GCTGAGC              GC^T[ACGT]AGC
DpnII         390  GATC                 ^GATC
DsaI           77  CCACGG               C^C[GA][CT]GG
EagI           80  CGGCCG               C^GGCCG
EarI          826  GAAGAG               CTCTTC(1/4)
EclXI          80  CGGCCG               C^GGCCG
Eco52I         80  CGGCCG               C^GGCCG
EcoT22I       635  ATGCAT               ATGCA^T
HinfI         519  GAGTC                G^A[ACGT]TC
KpnI           12  GGTACC               GGTAC^C
Ksp632I       826  GAAGAG               CTCTTC(1/4)
MaeIII         38  GTGAC                ^GT[ACGT]AC
MboI          390  GATC                 ^GATC
MluNI         780  TGGCCA               TGG^CCA
MroI           70  TCCGGA               T^CCGGA
MscI          780  TGGCCA               TGG^CCA
NciI          144  CCGGG                CC^[GC]GG
NdeII         390  GATC                 ^GATC
NsiI          635  ATGCAT               ATGCA^T
NspI          235  GCATGC               [GA]CATG^[CT]
PflMI         342  CCAAGAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          519  GAGTC                GAGTC(4/5)
PstI          161  CTGCAG               CTGCA^G
SacI           66  GAGCTC               GAGCT^C
Sau3AI        390  GATC                 ^GATC
SfcI          161  CTGCAG               C^T[GA][CT]AG
SphI          235  GCATGC               GCATG^C
TaqI           32  TCGA                 T^CGA
Tsp45I         38  GTGAC                ^GT[GC]AC
TthHB8I        32  TCGA                 T^CGA
Van91I        342  CCAAGAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          663  TCTAGA               T^CTAGA
XmnI          471  GAAGTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AciI            4  GCGG                 CCGC(-3/-1)
               22  GCGG                
AfaI           13  GTAC                 GT^AC
              690  GTAC                
AvaII          73  GGACC                G^G[AT]CC
              568  GGACC               
BanII          66  GAGCTC               G[GA]GC[CT]^C
              224  GAGCCC              
BfaI           52  CTAG                 C^TAG
              664  CTAG                
BmyI           66  GAGCTC               G[AGT]GC[ACT]^C
              224  GAGCCC              
BsaWI          70  TCCGGA               [AT]^CCGG[AT]
              242  ACCGGA              
BsiEI          80  CGGCCG               CG[GA][CT]^CG
              240  CGACCG              
Bsp1286I       66  GAGCTC               G[AGT]GC[ACT]^C
              224  GAGCCC              
BstF5I        172  CATCC                GGATG(2/0)
              503  CATCC               
Cac8I         235  GCATGC               GC[ACGT]^[ACGT]GC
              609  GCTTGC              
DdeI           64  CTGAG                C^T[ACGT]AG
              119  CTCAG               
EaeI           80  CGGCCG               [CT]^GGCC[GA]
              780  TGGCCA              
FokI          172  CATCC                GGATG(9/13)
              503  CATCC               
HindIII       397  AAGCTT               A^AGCTT
              607  AAGCTT              
MaeI           52  CTAG                 C^TAG
              664  CTAG                
MseI          194  TTAA                 T^TAA
              745  TTAA                
NlaIII        236  CATG                 CATG^
              444  CATG                
RsaI           13  GTAC                 GT^AC
              690  GTAC                
Tru9I         194  TTAA                 T^TAA
              745  TTAA                
Tsp509I       355  AATT                 ^AATT
              747  AATT                
TspRI         164  CAGTG                CA[GC]TG[ACGT][ACGT]^
              699  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AluI        BanI        BbsI        BbvI        BpuAI       
BsaJI       BseRI       BsiYI       BslI        BsrI        
BssKI       BstNI       Cfr13I      EcoRII      Fnu4HI      
HaeIII      HapII       HpaII       ItaI        MboII       
MnlI        MspI        MvaI        MwoI        NlaIV       
Sau96I      ScrFI       SfaNI       TseI        
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