Restriction map for
pF1KE0688
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII BalI BanII BbeI
BbrPI BbvCI BciVI BclI BglI
BlpI BpmI Bpu1102I BsaAI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV FbaI FseI FspI HindIII
HpaI KasI KpnI KspI MaeII
MfeI MluI MluNI MroI MscI
MslI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcyI 841 GACGCC G[GA]^CG[CT]C
AflII 898 CTTAAG C^TTAAG
AspHI 783 GAGCAC G[AT]GC[AT]^C
AvaI 566 CCCGAG C^[CT]CG[GA]G
AvrII 18 CCTAGG C^CTAGG
BamHI 240 GGATCC G^GATCC
BanI 1036 GGTGCC G^G[CT][GA]CC
BcgI 824 GCAAGATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 744 CCGGG CC^[GC]GG
BfrI 898 CTTAAG C^TTAAG
BglII 661 AGATCT A^GATCT
BlnI 18 CCTAGG C^CTAGG
BsaBI 874 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 841 GACGCC G[GA]^CG[CT]C
BsaWI 670 ACCGGA [AT]^CCGG[AT]
BsiHKAI 783 GAGCAC G[AT]GC[AT]^C
BsmFI 3 GTCCC GGGAC(10/14)
BsoBI 566 CCCGAG C^[CT]CG[GA]G
BsrDI 522 CATTGC GCAATG(2/0)
BsrFI 194 ACCGGC [GA]^CCGG[CT]
BstXI 533 CCACTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 812 GCAAGC GC[ACGT]^[ACGT]GC
Cfr10I 194 ACCGGC [GA]^CCGG[CT]
DraII 776 AGGGCCT [GA]G^G[ACGT]CC[CT]
EarI 583 GAAGAG CTCTTC(1/4)
EcoO109I 776 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 857 ATGCAT ATGCA^T
HaeII 338 AGCGCC [GA]GCGC^[CT]
HgaI 841 GACGC GACGC(5/10)
Hin1I 841 GACGCC G[GA]^CG[CT]C
HincII 1108 GTTGAC GT[CT]^[GA]AC
HindII 1108 GTTGAC GT[CT]^[GA]AC
HphI 93 GGTGA GGTGA(8/7)
Ksp632I 583 GAAGAG CTCTTC(1/4)
MamI 874 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI 744 CCGGG CC^[GC]GG
NsiI 857 ATGCAT ATGCA^T
PflMI 753 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 227 CAGCTG CAG^CTG
TaqI 600 TCGA T^CGA
Tsp45I 35 GTGAC ^GT[GC]AC
TthHB8I 600 TCGA T^CGA
Van91I 753 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AvaII 56 GGTCC G^G[AT]CC
673 GGACC
BfaI 19 CTAG C^TAG
677 CTAG
BsaI 28 GAGACC GGTCTC(1/5)
447 GAGACC
BseRI 183 CTCCTC GAGGAG(10/8)
492 GAGGAG
BstF5I 860 CATCC GGATG(2/0)
954 GGATG
BstNI 753 CCAGG CC^[AT]GG
1033 CCTGG
CfoI 339 GCGC GCG^C
715 GCGC
EcoRII 753 CCAGG ^CC[AT]GG
1033 CCTGG
EcoT14I 18 CCTAGG C^C[AT][AT]GG
977 CCAAGG
FauI 366 GCGGG CCCGC(4/6)
943 GCGGG
FokI 860 CATCC GGATG(9/13)
954 GGATG
HaeIII 751 GGCC GG^CC
778 GGCC
HhaI 339 GCGC GCG^C
715 GCGC
HinP1I 339 GCGC G^CGC
715 GCGC
MaeI 19 CTAG C^TAG
677 CTAG
MaeIII 35 GTGAC ^GT[ACGT]AC
561 GTTAC
MseI 899 TTAA T^TAA
1097 TTAA
MvaI 753 CCAGG CC^[AT]GG
1033 CCTGG
NspI 219 GCATGT [GA]CATG^[CT]
1150 GCATGT
PleI 37 GACTC GAGTC(4/5)
589 GACTC
PstI 76 CTGCAG CTGCA^G
263 CTGCAG
SfaNI 856 GATGC GCATC(5/9)
859 GCATC
SfcI 76 CTGCAG C^T[GA][CT]AG
263 CTGCAG
SmlI 591 CTCAAG C^T[CT][GA]AG
898 CTTAAG
StyI 18 CCTAGG C^C[AT][AT]GG
977 CCAAGG
Tru9I 899 TTAA T^TAA
1097 TTAA
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# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI BbsI BbvI BmyI
BpuAI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstYI Cfr13I
DdeI DpnII Fnu4HI HapII HinfI
HpaII ItaI MboI MboII MflI
MnlI MspA1I MspI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI TfiI TseI Tsp509I TspRI
XhoII