Restriction map for pF1KE0656
 (Circular) (Six-base) MAPSORT of: pF1KE0656.seq  Check: 9027  from: 1  to: 4277



pF1KE0656  4277 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1052    1052 
   Size:       4277
 
AccI GT'mk_AC

Cuts at:   1315    1315 
   Size:       4277
 
Acc65I G'GTAC_C

Cuts at:   1293    1293 
   Size:       4277
 
AclI AA'CG_TT

Cuts at:   4117    4117 
   Size:       4277
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    517     811     907     944    2102    2534    3281    3549     517 
   Size:        294      96      37    1158     432     747     268    1245

  Fragments arranged by size: 

                1245    1158     747     432     294     268      96      37
            
 
AflIII A'CryG_T

Cuts at:    140     142     962    1629    2733    3710     140 
   Size:          2     820     667    1104     977     707

  Fragments arranged by size: 

                1104     977     820     707     667       2
 
AgeI A'CCGG_T

Cuts at:   2665    2665 
   Size:       4277
 
AleI CACnn'nnGTG

Cuts at:    473     473 
   Size:       4277
 
AlwNI CAG_nnn'CTG

Cuts at:   1582    3149    1582 
   Size:       1567    2710
 
ApaLI G'TGCA_C

Cuts at:   3047    3047 
   Size:       4277
 
ApoI r'AATT_y

Cuts at:    133     228     910    1084    1281    3630    3886     133 
   Size:         95     682     174     197    2349     256     524

  Fragments arranged by size: 

                2349     682     524     256     197     174      95
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4277
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4277
 
AvaI C'yCGr_G

Cuts at:   1297    1297 
   Size:       4277
 
AvrII C'CTAG_G

Cuts at:    441     441 
   Size:       4277
 
BamHI G'GATC_C

Cuts at:   1302    1302 
   Size:       4277
 
BanI G'GyrC_C

Cuts at:   1293    1707    1955    1990    1293 
   Size:        414     248      35    3580

  Fragments arranged by size: 

                3580     414     248      35
 
BanII G_rGCy'C

Cuts at:   1291    2321    1291 
   Size:       1030    3247
 
BbeI G_GCGC'C

Cuts at:   1711    1959    1711 
   Size:        248    4029
 
BciVI GTATCCnnnnn_n'

Cuts at:   2175    2936    4277    2175 
   Size:        761    1341    2175

  Fragments arranged by size: 

                2175    1341     761
 
BclI T'GATC_A

Cuts at:    372    1797     372 
   Size:       1425    2852
 
BfrBI ATG'CAT

Cuts at:    762     762 
   Size:       4277
 
BglII A'GATC_T

Cuts at:    250    1792    3829     250 
   Size:       1542    2037     698

  Fragments arranged by size: 

                2037    1542     698
 
BlpI GC'TnA_GC

Cuts at:    827    1389     827 
   Size:        562    3715
 
Bme1580I G_kGCm'C

Cuts at:   1902    1995    3051    1902 
   Size:         93    1056    3128

  Fragments arranged by size: 

                3128    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1489    1570    1905    4049    1489 
   Size:         81     335    2144    1717

  Fragments arranged by size: 

                2144    1717     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1137    2644    1137 
   Size:       1507    2770
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    184    1194     184 
   Size:       1010    3267
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1159    1453    2332    2824    3122    1159 
   Size:        294     879     492     298    2314

  Fragments arranged by size: 

                2314     879     492     298     294
 
BsaI GGTCTCn'nnnn_

Cuts at:   3981    3981 
   Size:       4277
 
BsaAI yAC'GTr

Cuts at:    143    1630     143 
   Size:       1487    2790
 
BsaBI GATnn'nnATC

Cuts at:      6    1791    3828       6 
   Size:       1785    2037     455

  Fragments arranged by size: 

                2037    1785     455
 
BsaHI Gr'CG_yC

Cuts at:   1708    1956    2658    4154    1708 
   Size:        248     702    1496    1831

  Fragments arranged by size: 

                1831    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1466    1987    2665    2939    3086    3497    1466 
   Size:        521     678     274     147     411    2246

  Fragments arranged by size: 

                2246     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    265     295     265 
   Size:         30    4247
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    347     461     493     559    1117     347 
   Size:        114      32      66     558    3507

  Fragments arranged by size: 

                3507     558     114      66      32
 
BseYI C'CCAG_C

Cuts at:   1702    2460    3037    3533    1702 
   Size:        758     577     496    2446

  Fragments arranged by size: 

                2446     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    207     207 
   Size:       4277
 
BsiEI CG_ry'CG

Cuts at:    104    1819    1865    3073     104 
   Size:       1715      46    1208    1308

  Fragments arranged by size: 

                1715    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1291    2069    2259    3051    1291 
   Size:        778     190     792    2517

  Fragments arranged by size: 

                2517     792     778     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    475    1030     475 
   Size:        555    3722
 
Bsp1286I G_dGCh'C

Cuts at:   1291    1902    1995    2069    2259    2321    3051    1291 
   Size:        611      93      74     190      62     730    2517

  Fragments arranged by size: 

                2517     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   3497    3497 
   Size:       4277
 
BspHI T'CATG_A

Cuts at:    569     728    3453     569 
   Size:        159    2725    1393

  Fragments arranged by size: 

                2725    1393     159
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1052    1327    1541    1843    2224    1052 
   Size:        275     214     302     381    3105

  Fragments arranged by size: 

                3105     381     302     275     214
 
BsrBI CCG'CTC

Cuts at:     50     403    2569    2623    4107    4272      50 
   Size:        353    2166      54    1484     165      55

  Fragments arranged by size: 

                2166    1484     353     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2189    2189 
   Size:       4277
 
BsrFI r'CCGG_y

Cuts at:   2275    2456    2665    2275 
   Size:        181     209    3887

  Fragments arranged by size: 

                3887     209     181
 
BsrGI T'GTAC_A

Cuts at:    770     770 
   Size:       4277
 
BssSI C'ACGA_G

Cuts at:   2548    2906    2548 
   Size:        358    3919
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1654    1654 
   Size:       4277
 
BstBI TT'CG_AA

Cuts at:   2638    2638 
   Size:       4277
 
BstYI r'GATC_y

Cuts at:      1     250    1302    1769    1792    2127    2373    3385    3471 
   Size:        249    1052     467      23     335     246    1012      86

Cuts at:   3471    3483    3829       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1052    1012     467     449     346     335     249     246
                  86      23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    966    2227    2408    2571     966 
   Size:       1261     181     163    2672

  Fragments arranged by size: 

                2672    1261     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1520    1520 
   Size:       4277
 
ClaI AT'CG_AT

Cuts at:    686     686 
   Size:       4277
 
DraI TTT'AAA

Cuts at:   1276    1276 
   Size:       4277
 
DrdI GACnn_nn'nnGTC

Cuts at:   1983    2841    1983 
   Size:        858    3419
 
EaeI y'GGCC_r

Cuts at:    459     485     857    1862    2036    2427    2454    3799     459 
   Size:         26     372    1005     174     391      27    1345     937

  Fragments arranged by size: 

                1345    1005     937     391     372     174      27      26
            
 
EagI C'GGCC_G

Cuts at:   1862    1862 
   Size:       4277
 
EarI CTCTTCn'nnn_

Cuts at:    115     188    1138    2300    2510     115 
   Size:         73     950    1162     210    1882

  Fragments arranged by size: 

                1882    1162     950     210      73
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2485    2795    2941    4087    2485 
   Size:        310     146    1146    2675

  Fragments arranged by size: 

                2675    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1289    1289 
   Size:       4277
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    184     517     811     907     944    1194    2102    2534    3281 
   Size:        333     294      96      37     250     908     432     747

Cuts at:   3281    3549     184 
   Size:        268     912

  Fragments arranged by size: 

                 912     908     747     432     333     294     268     250
                  96      37
 
EcoO109I rG'GnC_Cy

Cuts at:   1416    1416 
   Size:       4277
 
EcoRI G'AATT_C

Cuts at:    133    1281     133 
   Size:       1148    3129
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    912    2296     912 
   Size:       1384    2893
 
FspI TGC'GCA

Cuts at:   2058    2058 
   Size:       4277
 
HaeII r_GCGC'y

Cuts at:   1711    1959    2981    3960    1711 
   Size:        248    1022     979    2028

  Fragments arranged by size: 

                2028    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    235     265     267     297    2119    2149    2151    2181     235 
   Size:         30       2      30    1822      30       2      30    2331

  Fragments arranged by size: 

                2331    1822      30      30      30      30       2       2
            
 
HincII GTy'rAC

Cuts at:    345     408    1316     345 
   Size:         63     908    3306

  Fragments arranged by size: 

                3306     908      63
 
HindIII A'AGCT_T

Cuts at:   1126    1126 
   Size:       4277
 
KasI G'GCGC_C

Cuts at:   1707    1955    1707 
   Size:        248    4029
 
KpnI G_GTAC'C

Cuts at:   1297    1297 
   Size:       4277
 
MluI A'CGCG_T

Cuts at:   3710    3710 
   Size:       4277
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2948    3132    2948 
   Size:        184    4093
 
MscI TGG'CCA

Cuts at:    859    2038     859 
   Size:       1179    3098
 
MslI CAynn'nnrTG

Cuts at:    473     961    2266    2393     473 
   Size:        488    1305     127    2357

  Fragments arranged by size: 

                2357    1305     488     127
 
MspA1I CmG'CkG

Cuts at:    162     579     603    1388    1702    2062    3075    3320     162 
   Size:        417      24     785     314     360    1013     245    1119

  Fragments arranged by size: 

                1119    1013     785     417     360     314     245      24
            
 
NaeI GCC'GGC

Cuts at:   2458    2458 
   Size:       4277
 
NarI GG'CG_CC

Cuts at:   1708    1956    1708 
   Size:        248    4029
 
NgoMIV G'CCGG_C

Cuts at:   2456    2456 
   Size:       4277
 
NsiI A_TGCA'T

Cuts at:    764     764 
   Size:       4277
 
NspI r_CATG'y

Cuts at:    762     966    1330    1830    2737     762 
   Size:        204     364     500     907    2302

  Fragments arranged by size: 

                2302     907     500     364     204
 
PciI A'CATG_T

Cuts at:    962    2733     962 
   Size:       1771    2506
 
PmeI GTTT'AAAC

Cuts at:   1276    1276 
   Size:       4277
 
PmlI CAC'GTG

Cuts at:    143     143 
   Size:       4277
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3416    3448    3416 
   Size:         32    4245
 
PsiI TTA'TAA

Cuts at:    149     149 
   Size:       4277
 
PstI C_TGCA'G

Cuts at:   1063    1324    2009    1063 
   Size:        261     685    3331

  Fragments arranged by size: 

                3331     685     261
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4277
 
PvuII CAG'CTG

Cuts at:    162     579     603    1702    2062     162 
   Size:        417      24    1099     360    2377

  Fragments arranged by size: 

                2377    1099     417     360      24
 
RsrII CG'GwC_CG

Cuts at:   2472    2472 
   Size:       4277
 
SacI G_AGCT'C

Cuts at:   1291    1291 
   Size:       4277
 
SalI G'TCGA_C

Cuts at:   1314    1314 
   Size:       4277
 
SapI GCTCTTCn'nnn_

Cuts at:   2300    2510    2300 
   Size:        210    4067
 
SbfI CC_TGCA'GG

Cuts at:   1063    1324    1063 
   Size:        261    4016
 
ScaI AGT'ACT

Cuts at:   3794    3794 
   Size:       4277
 
SfcI C'TryA_G

Cuts at:     33    1059    1320    2005    2998    3189      33 
   Size:       1026     261     685     993     191    1121

  Fragments arranged by size: 

                1121    1026     993     685     261     191
 
SfoI GGC'GCC

Cuts at:   1709    1957    1709 
   Size:        248    4029
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4277
 
SmaI CCC'GGG

Cuts at:   1299    1299 
   Size:       4277
 
SmlI C'TyrA_G

Cuts at:   1174    1432    2347    2839    3101    1174 
   Size:        258     915     492     262    2350

  Fragments arranged by size: 

                2350     915     492     262     258
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4277
 
SphI G_CATG'C

Cuts at:   1330    1830    1330 
   Size:        500    3777
 
StyI C'CwwG_G

Cuts at:    441    1109    1411    1765     441 
   Size:        668     302     354    2953

  Fragments arranged by size: 

                2953     668     354     302
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1009    1860    1993    2452    2661    1009 
   Size:        851     133     459     209    2625

  Fragments arranged by size: 

                2625     851     459     209     133
 
TatI w'GTAC_w

Cuts at:    770    3792     770 
   Size:       3022    1255
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3794    3826    3794 
   Size:         32    4245
 
Tth111I GACn'n_nGTC

Cuts at:    634    2074     634 
   Size:       1440    2837
 
XbaI T'CTAG_A

Cuts at:   1308    1308 
   Size:       4277
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1772    1772 
   Size:       4277
 
XmaI C'CCGG_G

Cuts at:   1297    1297 
   Size:       4277
 
XmnI GAAnn'nnTTC

Cuts at:   1085    1085 
   Size:       4277
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    AvrII    BamHI
     BanI    BanII     BbeI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI    BssSI   BstAPI    BstBI    BstYI    BtgZI     BtsI     ClaI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI   NgoMIV     NsiI     NspI     PciI     PmeI     PmlI     PpiI
     PsiI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AlfI     AloI     ApaI     AscI
     BaeI     BbsI    BbvCI     BcgI     BglI    BmgBI     BmtI   Bpu10I
    BsiWI     BsmI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI     HpaI     MfeI
     NcoI     NdeI     NheI     NotI     NruI     PacI     PasI    PflMI
     PfoI    PpuMI    PshAI   PspOMI    PspXI     PsrI    SacII    SanDI
    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI
     XhoI     ZraI
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