Restriction map for pF1KE0617
 (Circular) (Six-base) MAPSORT of: pF1KE0617.seq  Check: 5206  from: 1  to: 3662



pF1KE0617  3662 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AccI GT'mk_AC

Cuts at:    549     700     549 
   Size:        151    3511
 
Acc65I G'GTAC_C

Cuts at:    678     678 
   Size:       3662
 
AclI AA'CG_TT

Cuts at:   3502    3502 
   Size:       3662
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    197     320    1487    1919    2666    2934     197 
   Size:        123    1167     432     747     268     925

  Fragments arranged by size: 

                1167     925     747     432     268     123
 
AflIII A'CryG_T

Cuts at:    134    1014    2118    3095     134 
   Size:        880    1104     977     701

  Fragments arranged by size: 

                1104     977     880     701
 
AgeI A'CCGG_T

Cuts at:   2050    2050 
   Size:       3662
 
AhdI GACnn_n'nnGTC

Cuts at:    171     171 
   Size:       3662
 
AlwNI CAG_nnn'CTG

Cuts at:    967    2534     967 
   Size:       1567    2095
 
ApaLI G'TGCA_C

Cuts at:   2432    2432 
   Size:       3662
 
ApoI r'AATT_y

Cuts at:    193     666    3015    3271     193 
   Size:        473    2349     256     584

  Fragments arranged by size: 

                2349     584     473     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3662
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3662
 
AvaI C'yCGr_G

Cuts at:    682     682 
   Size:       3662
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    444     477     444 
   Size:         33    3629
 
BamHI G'GATC_C

Cuts at:    687     687 
   Size:       3662
 
BanI G'GyrC_C

Cuts at:    678    1092    1340    1375     678 
   Size:        414     248      35    2965

  Fragments arranged by size: 

                2965     414     248      35
 
BanII G_rGCy'C

Cuts at:    676    1706     676 
   Size:       1030    2632
 
BbeI G_GCGC'C

Cuts at:   1096    1344    1096 
   Size:        248    3414
 
BbsI GAAGACnn'nnnn_

Cuts at:    185     308     567     185 
   Size:        123     259    3280

  Fragments arranged by size: 

                3280     259     123
 
BciVI GTATCCnnnnn_n'

Cuts at:   1560    2321    3662    1560 
   Size:        761    1341    1560

  Fragments arranged by size: 

                1560    1341     761
 
BclI T'GATC_A

Cuts at:   1182    1182 
   Size:       3662
 
BglII A'GATC_T

Cuts at:   1177    3214    1177 
   Size:       2037    1625
 
BlpI GC'TnA_GC

Cuts at:    774     774 
   Size:       3662
 
Bme1580I G_kGCm'C

Cuts at:   1287    1380    2436    1287 
   Size:         93    1056    2513

  Fragments arranged by size: 

                2513    1056      93
 
BmgBI CAC'GTC

Cuts at:    137     137 
   Size:       3662
 
BmrI ACTGGGnnnn_n'

Cuts at:    615     874     955    1290    3434     615 
   Size:        259      81     335    2144     843

  Fragments arranged by size: 

                2144     843     335     259      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2029    2029 
   Size:       3662
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    838    1717    2209    2507     838 
   Size:        879     492     298    1993

  Fragments arranged by size: 

                1993     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3366    3366 
   Size:       3662
 
BsaAI yAC'GTr

Cuts at:   1015    1015 
   Size:       3662
 
BsaBI GATnn'nnATC

Cuts at:      6    1176    3213       6 
   Size:       1170    2037     455

  Fragments arranged by size: 

                2037    1170     455
 
BsaHI Gr'CG_yC

Cuts at:   1093    1341    2043    3539    1093 
   Size:        248     702    1496    1216

  Fragments arranged by size: 

                1496    1216     702     248
 
BsaWI w'CCGG_w

Cuts at:    851    1372    2050    2324    2471    2882     851 
   Size:        521     678     274     147     411    1631

  Fragments arranged by size: 

                1631     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    501     501 
   Size:       3662
 
BseYI C'CCAG_C

Cuts at:   1087    1845    2422    2918    1087 
   Size:        758     577     496    1831

  Fragments arranged by size: 

                1831     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1204    1250    2458     104 
   Size:       1100      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1100      46
 
BsiHKAI G_wGCw'C

Cuts at:    676    1454    1644    2436     676 
   Size:        778     190     792    1902

  Fragments arranged by size: 

                1902     792     778     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    143     143 
   Size:       3662
 
Bsp1286I G_dGCh'C

Cuts at:    676    1287    1380    1454    1644    1706    2436     676 
   Size:        611      93      74     190      62     730    1902

  Fragments arranged by size: 

                1902     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   2882    2882 
   Size:       3662
 
BspHI T'CATG_A

Cuts at:   2838    2838 
   Size:       3662
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    128     712     926    1228    1609     128 
   Size:        584     214     302     381    2181

  Fragments arranged by size: 

                2181     584     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1954    2008    3492    3657      50 
   Size:       1904      54    1484     165      55

  Fragments arranged by size: 

                1904    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1574    1574 
   Size:       3662
 
BsrFI r'CCGG_y

Cuts at:   1660    1841    2050    1660 
   Size:        181     209    3272

  Fragments arranged by size: 

                3272     209     181
 
BsrGI T'GTAC_A

Cuts at:    419     419 
   Size:       3662
 
BssSI C'ACGA_G

Cuts at:   1933    2291    1933 
   Size:        358    3304
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1039    1039 
   Size:       3662
 
BstBI TT'CG_AA

Cuts at:   2023    2023 
   Size:       3662
 
BstYI r'GATC_y

Cuts at:      1     687    1154    1177    1512    1758    2770    2856    2868 
   Size:        686     467      23     335     246    1012      86      12

Cuts at:   2868    3214       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     686     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    565     565 
   Size:       3662
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1612    1793    1956    1612 
   Size:        181     163    3318

  Fragments arranged by size: 

                3318     181     163
 
BtsI GCAGTG_nn'

Cuts at:    905     905 
   Size:       3662
 
DraI TTT'AAA

Cuts at:    661     661 
   Size:       3662
 
DrdI GACnn_nn'nnGTC

Cuts at:   1368    2226    1368 
   Size:        858    2804
 
EaeI y'GGCC_r

Cuts at:    158    1247    1421    1812    1839    3184     158 
   Size:       1089     174     391      27    1345     636

  Fragments arranged by size: 

                1345    1089     636     391     174      27
 
EagI C'GGCC_G

Cuts at:   1247    1247 
   Size:       3662
 
EarI CTCTTCn'nnn_

Cuts at:   1685    1895    1685 
   Size:        210    3452
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1870    2180    2326    3472    1870 
   Size:        310     146    1146    2060

  Fragments arranged by size: 

                2060    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    674     674 
   Size:       3662
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    197     320    1487    1919    2666    2934     197 
   Size:        123    1167     432     747     268     925

  Fragments arranged by size: 

                1167     925     747     432     268     123
 
EcoO109I rG'GnC_Cy

Cuts at:    801     801 
   Size:       3662
 
EcoRI G'AATT_C

Cuts at:    666     666 
   Size:       3662
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    521    1681     521 
   Size:       1160    2502
 
FspI TGC'GCA

Cuts at:   1443    1443 
   Size:       3662
 
HaeII r_GCGC'y

Cuts at:   1096    1344    2366    3345    1096 
   Size:        248    1022     979    1413

  Fragments arranged by size: 

                1413    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    157     189     372     404    1504    1534    1536    1566     157 
   Size:         32     183      32    1100      30       2      30    2253

  Fragments arranged by size: 

                2253    1100     183      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    701     701 
   Size:       3662
 
KasI G'GCGC_C

Cuts at:   1092    1340    1092 
   Size:        248    3414
 
KpnI G_GTAC'C

Cuts at:    682     682 
   Size:       3662
 
MluI A'CGCG_T

Cuts at:   3095    3095 
   Size:       3662
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    611    2333    2517     611 
   Size:       1722     184    1756

  Fragments arranged by size: 

                1756    1722     184
 
MscI TGG'CCA

Cuts at:   1423    1423 
   Size:       3662
 
MslI CAynn'nnrTG

Cuts at:   1651    1778    1651 
   Size:        127    3535
 
MspA1I CmG'CkG

Cuts at:    611     773    1087    1447    2460    2705     611 
   Size:        162     314     360    1013     245    1568

  Fragments arranged by size: 

                1568    1013     360     314     245     162
 
NaeI GCC'GGC

Cuts at:   1843    1843 
   Size:       3662
 
NarI GG'CG_CC

Cuts at:   1093    1341    1093 
   Size:        248    3414
 
NgoMIV G'CCGG_C

Cuts at:   1841    1841 
   Size:       3662
 
NspI r_CATG'y

Cuts at:    715    1215    2122     715 
   Size:        500     907    2255

  Fragments arranged by size: 

                2255     907     500
 
PasI CC'CwG_GG

Cuts at:    366     366 
   Size:       3662
 
PciI A'CATG_T

Cuts at:   2118    2118 
   Size:       3662
 
PmeI GTTT'AAAC

Cuts at:    661     661 
   Size:       3662
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2801    2833    2801 
   Size:         32    3630
 
PstI C_TGCA'G

Cuts at:    610     709    1394     610 
   Size:         99     685    2878

  Fragments arranged by size: 

                2878     685      99
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3662
 
PvuII CAG'CTG

Cuts at:    611    1087    1447     611 
   Size:        476     360    2826

  Fragments arranged by size: 

                2826     476     360
 
RsrII CG'GwC_CG

Cuts at:   1857    1857 
   Size:       3662
 
SacI G_AGCT'C

Cuts at:    676     676 
   Size:       3662
 
SalI G'TCGA_C

Cuts at:    699     699 
   Size:       3662
 
SapI GCTCTTCn'nnn_

Cuts at:   1685    1895    1685 
   Size:        210    3452
 
SbfI CC_TGCA'GG

Cuts at:    709     709 
   Size:       3662
 
ScaI AGT'ACT

Cuts at:   3179    3179 
   Size:       3662
 
SexAI A'CCwGG_T

Cuts at:    169     169 
   Size:       3662
 
SfcI C'TryA_G

Cuts at:     33     212     519     606     705    1390    2383    2574      33 
   Size:        179     307      87      99     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     307     191     179      99      87
            
 
SfoI GGC'GCC

Cuts at:   1094    1342    1094 
   Size:        248    3414
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3662
 
SmaI CCC'GGG

Cuts at:    684     684 
   Size:       3662
 
SmlI C'TyrA_G

Cuts at:    817    1732    2224    2486     817 
   Size:        915     492     262    1993

  Fragments arranged by size: 

                1993     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3662
 
SphI G_CATG'C

Cuts at:    715    1215     715 
   Size:        500    3162
 
StyI C'CwwG_G

Cuts at:    401     796    1150     401 
   Size:        395     354    2913

  Fragments arranged by size: 

                2913     395     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    491     567    1245    1378    1837    2046     491 
   Size:         76     678     133     459     209    2107

  Fragments arranged by size: 

                2107     678     459     209     133      76
 
TatI w'GTAC_w

Cuts at:    419     623    3177     419 
   Size:        204    2554     904

  Fragments arranged by size: 

                2554     904     204
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    237     269     313     345    3179    3211     237 
   Size:         32      44      32    2834      32     688

  Fragments arranged by size: 

                2834     688      44      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1459    1459 
   Size:       3662
 
XbaI T'CTAG_A

Cuts at:    463     693     463 
   Size:        230    3432
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    608    1157     608 
   Size:        549    3113
 
XmaI C'CCGG_G

Cuts at:    682     682 
   Size:       3662
 
XmnI GAAnn'nnTTC

Cuts at:    582     582 
   Size:       3662
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BciVI     BclI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PasI
     PciI     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AleI     AlfI     AloI     ApaI
     AscI    AvrII    BbvCI     BcgI    BfrBI     BglI     BmtI     BpmI
   Bpu10I    BsaXI     BsgI    BsiWI     BsmI   BssHII   BstEII    BstXI
  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     PacI    PflMI     PfoI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI     SfiI    SgrAI
    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com