Restriction map for pF1KE0614
##################################
# NO CUT ENZYMES (  155 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BamHI       BanII       BbeI        
BbrPI       BbvCI       BclI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstUI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DrdI        
DsaI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        FspI        HgaI        
Hin1I       HindIII     HpaI        KasI        KpnI        
Ksp632I     KspI        MamI        MfeI        MluI        
MroI        MseI        MspA1I      MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tru9I       Tth111I     XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AflIII        842  ACGTGT               A^C[GA][CT]GT
AlwI          972  GATCC                GGATC(4/5)
Asp700I       879  GAAATTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI          594  GGCACC               G^G[CT][GA]CC
BcgI           81  CGAGTCCATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         750  GGATAC               GTATCC(6/5)
BcnI          737  CCCGG                CC^[GC]GG
BfaI          838  CTAG                 C^TAG
BpmI         1061  CTGGAG               CTGGAG(16/14)
BsaJI         736  CCCCGG               C^C[ACGT][ACGT]GG
BsmFI         159  GGGAC                GGGAC(10/14)
BsrBI        1026  CCGCTC               CCGCTC(-3/-3)
BstEII        645  GGTGACC              G^GT[ACGT]ACC
BstNI         817  CCTGG                CC^[AT]GG
BstPI         645  GGTGACC              G^GT[ACGT]ACC
BstYI         971  AGATCC               [GA]^GATC[CT]
DpnII         972  GATC                 ^GATC
DraIII       1081  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI         366  CCTTCGTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       645  GGTGACC              G^GT[ACGT]ACC
EcoRI         901  GAATTC               G^AATTC
EcoRII        817  CCTGG                ^CC[AT]GG
FauI          486  GCGGG                CCCGC(4/6)
HaeII         955  AGCGCC               [GA]GCGC^[CT]
HincII        226  GTCAAC               GT[CT]^[GA]AC
HindII        226  GTCAAC               GT[CT]^[GA]AC
MaeI          838  CTAG                 C^TAG
MboI          972  GATC                 ^GATC
MflI          971  AGATCC               [GA]^GATC[CT]
MvaI          817  CCTGG                CC^[AT]GG
NciI          737  CCCGG                CC^[GC]GG
NdeII         972  GATC                 ^GATC
NspI          455  GCATGC               [GA]CATG^[CT]
PpuMI         327  AGGACCC              [GA]G^G[AT]CC[CT]
PshAI         774  GACTGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          278  CTGCAG               CTGCA^G
Sau3AI        972  GATC                 ^GATC
SphI          455  GCATGC               GCATG^C
TaqI          431  TCGA                 T^CGA
TthHB8I       431  TCGA                 T^CGA
XbaI          837  TCTAGA               T^CTAGA
XcmI          632  CCATTTTCAACTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         971  AGATCC               [GA]^GATC[CT]
XmnI          879  GAAATTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcsI          880  AAATTC               [GA]^AATT[CT]
              901  GAATTC              
AluI          149  AGCT                 AG^CT
              461  AGCT                
ApoI          880  AAATTC               [GA]^AATT[CT]
              901  GAATTC              
AvaII         328  GGACC                G^G[AT]CC
              733  GGACC               
BsmAI         342  GAGAC                GTCTC(1/5)
              717  GTCTC               
BsrDI          87  CATTGC               GCAATG(2/0)
             1073  GCAATG              
BsrFI         482  ACCGGC               [GA]^CCGG[CT]
             1003  ACCGGC              
BssKI         737  CCCGG                ^CC[ACGT]GG
              817  CCTGG               
BstF5I         21  CATCC                GGATG(2/0)
              473  GGATG               
CfoI          956  GCGC                 GCG^C
              987  GCGC                
Cfr10I        482  ACCGGC               [GA]^CCGG[CT]
             1003  ACCGGC              
DdeI          618  CTTAG                C^T[ACGT]AG
             1020  CTCAG               
FokI           21  CATCC                GGATG(9/13)
              473  GGATG               
HhaI          956  GCGC                 GCG^C
              987  GCGC                
HinP1I        956  GCGC                 G^CGC
              987  GCGC                
HphI          153  GGTGA                GGTGA(8/7)
              645  GGTGA               
MaeII         842  ACGT                 A^CGT
              863  ACGT                
PflMI         125  CCACAGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1080  CCACTCTGTGG         
ScrFI         737  CCCGG                CC^[ACGT]GG
              817  CCTGG               
SfaNI         117  GCATC                GCATC(5/9)
              400  GCATC               
SfcI          278  CTGCAG               C^T[GA][CT]AG
              603  CTACAG              
TfiI          167  GAATC                G^A[AT]TC
              744  GAATC               
Van91I        125  CCACAGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1080  CCACTCTGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AciI        BalI        BbsI        BbvI        BpuAI       
BsiYI       BslI        BsrI        BstXI       Cac8I       
Cfr13I      DraII       EaeI        EcoO109I    Fnu4HI      
HaeIII      HapII       HinfI       HpaII       ItaI        
MaeIII      MboII       MluNI       MnlI        MscI        
MslI        MspI        MwoI        NlaIII      NlaIV       
PleI        Sau96I      TseI        Tsp45I      Tsp509I     
TspRI       
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