Restriction map for pF1KE0447
##################################
# NO CUT ENZYMES (  171 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AcsI        
AfaI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HgaI        HindIII     HpaI        KasI        
KpnI        KspI        MaeI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MslI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        TfiI        Tsp509I     Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AatII         117  GACGTC               GACGT^C
AcyI          117  GACGTC               G[GA]^CG[CT]C
Asp700I       243  GAAGCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI          88  GAGCAC               G[AT]GC[AT]^C
BanI          168  GGCACC               G^G[CT][GA]CC
BbvI          511  GCAGC                GCAGC(8/12)
BpmI          342  CTCCAG               CTGGAG(16/14)
BsaHI         117  GACGTC               G[GA]^CG[CT]C
BsaI          453  GGTCTC               GGTCTC(1/5)
BsaWI         365  ACCGGA               [AT]^CCGG[AT]
BsiHKAI        88  GAGCAC               G[AT]GC[AT]^C
BsmAI         454  GTCTC                GTCTC(1/5)
BsmFI         134  GTCCC                GGGAC(10/14)
DraII         422  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EaeI          148  CGGCCA               [CT]^GGCC[GA]
EcoNI         207  CCTGGAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      422  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       442  CCAAGG               C^C[AT][AT]GG
FauI          165  GCGGG                CCCGC(4/6)
HaeII         317  AGCGCC               [GA]GCGC^[CT]
HapII         366  CCGG                 C^CGG
Hin1I         117  GACGTC               G[GA]^CG[CT]C
HincII        229  GTCAAC               GT[CT]^[GA]AC
HindII        229  GTCAAC               GT[CT]^[GA]AC
HpaII         366  CCGG                 C^CGG
MaeII         118  ACGT                 A^CGT
MaeIII        505  GTCAC                ^GT[ACGT]AC
MseI          272  TTAA                 T^TAA
MspI          366  CCGG                 C^CGG
PpuMI         422  GGGTCCT              [GA]G^G[AT]CC[CT]
SapI          313  GAAGAGC              GCTCTTC(1/4)
SfaNI         201  GATGC                GCATC(5/9)
StyI          442  CCAAGG               C^C[AT][AT]GG
Tru9I         272  TTAA                 T^TAA
TseI          511  GCAGC                G^C[AT]GC
Tsp45I        505  GTCAC                ^GT[GC]AC
XcmI          321  CCAGGATGAACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          243  GAAGCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccI          120  GTCTAC               GT^[AC][GT]AC
              382  GTAGAC              
AluI          175  AGCT                 AG^CT
              375  AGCT                
AvaII         423  GGTCC                G^G[AT]CC
              439  GGACC               
BamHI          26  GGATCC               G^GATCC
              368  GGATCC              
BmyI           88  GAGCAC               G[AGT]GC[ACT]^C
              167  GGGCAC              
BsiYI         158  CCTTCCTGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              207  CCTGGAAAAGG         
BslI          158  CCTTCCTGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              207  CCTGGAAAAGG         
Bsp1286I       88  GAGCAC               G[AGT]GC[ACT]^C
              167  GGGCAC              
BsrI          187  ACTGG                ACTGG(1/-1)
               75  CCAGT               
BstYI          26  GGATCC               [GA]^GATC[CT]
              368  GGATCC              
Cac8I         165  GCGGGC               GC[ACGT]^[ACGT]GC
              498  GCCTGC              
EarI          313  GAAGAG               CTCTTC(1/4)
              490  GAAGAG              
HaeIII        149  GGCC                 GG^CC
              460  GGCC                
Ksp632I       313  GAAGAG               CTCTTC(1/4)
              490  GAAGAG              
MflI           26  GGATCC               [GA]^GATC[CT]
              368  GGATCC              
MspA1I        174  CAGCTG               C[AC]G^C[GT]G
              374  CAGCTG              
MwoI          461  GCCCTGAGCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              502  GCTGTCACCGC         
PvuII         174  CAGCTG               CAG^CTG
              374  CAGCTG              
TspRI         186  CACTG                CA[GC]TG[ACGT][ACGT]^
              429  CACTG               
XhoII          26  GGATCC               [GA]^GATC[CT]
              368  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AciI        AlwI        BsaJI       BssKI       BstF5I      
BstNI       CfoI        Cfr13I      DdeI        DpnII       
EcoRII      Fnu4HI      FokI        HhaI        HinP1I      
HinfI       HphI        ItaI        MboI        MboII       
MnlI        MvaI        NdeII       NlaIII      NlaIV       
PleI        Sau3AI      Sau96I      ScrFI       
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