Restriction map for pF1KE0447
 (Circular) (Six-base) MAPSORT of: pF1KE0447.seq  Check: 3854  from: 1  to: 3638



pF1KE0447  3638 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AatII G_ACGT'C

Cuts at:    229     229 
   Size:       3638
 
AccI GT'mk_AC

Cuts at:    229     491     676     229 
   Size:        262     185    3191

  Fragments arranged by size: 

                3191     262     185
 
Acc65I G'GTAC_C

Cuts at:    654     654 
   Size:       3638
 
AclI AA'CG_TT

Cuts at:   3478    3478 
   Size:       3638
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1463    1895    2642    2910    1463 
   Size:        432     747     268    2191

  Fragments arranged by size: 

                2191     747     432     268
 
AflIII A'CryG_T

Cuts at:    990    2094    3071     990 
   Size:       1104     977    1557

  Fragments arranged by size: 

                1557    1104     977
 
AgeI A'CCGG_T

Cuts at:   2026    2026 
   Size:       3638
 
AlwNI CAG_nnn'CTG

Cuts at:    943    2510     943 
   Size:       1567    2071
 
ApaLI G'TGCA_C

Cuts at:   2408    2408 
   Size:       3638
 
ApoI r'AATT_y

Cuts at:    642    2991    3247     642 
   Size:       2349     256    1033

  Fragments arranged by size: 

                2349    1033     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3638
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3638
 
AvaI C'yCGr_G

Cuts at:    658     658 
   Size:       3638
 
BamHI G'GATC_C

Cuts at:    134     476     663     134 
   Size:        342     187    3109

  Fragments arranged by size: 

                3109     342     187
 
BanI G'GyrC_C

Cuts at:    276     654    1068    1316    1351     276 
   Size:        378     414     248      35    2563

  Fragments arranged by size: 

                2563     414     378     248      35
 
BanII G_rGCy'C

Cuts at:    652    1682     652 
   Size:       1030    2608
 
BbeI G_GCGC'C

Cuts at:   1072    1320    1072 
   Size:        248    3390
 
BciVI GTATCCnnnnn_n'

Cuts at:   1536    2297    3638    1536 
   Size:        761    1341    1536

  Fragments arranged by size: 

                1536    1341     761
 
BclI T'GATC_A

Cuts at:   1158    1158 
   Size:       3638
 
BglII A'GATC_T

Cuts at:   1153    3190    1153 
   Size:       2037    1601
 
BlpI GC'TnA_GC

Cuts at:    750     750 
   Size:       3638
 
Bme1580I G_kGCm'C

Cuts at:    279    1263    1356    2412     279 
   Size:        984      93    1056    1505

  Fragments arranged by size: 

                1505    1056     984      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    850     931    1266    3410     850 
   Size:         81     335    2144    1078

  Fragments arranged by size: 

                2144    1078     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2005    2005 
   Size:       3638
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    435     435 
   Size:       3638
 
Bpu10I CC'TnA_GC

Cuts at:    572     572 
   Size:       3638
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    814    1693    2185    2483     814 
   Size:        879     492     298    1969

  Fragments arranged by size: 

                1969     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    567    3342     567 
   Size:       2775     863
 
BsaAI yAC'GTr

Cuts at:    991     991 
   Size:       3638
 
BsaBI GATnn'nnATC

Cuts at:      6    1152    3189       6 
   Size:       1146    2037     455

  Fragments arranged by size: 

                2037    1146     455
 
BsaHI Gr'CG_yC

Cuts at:    226    1069    1317    2019    3515     226 
   Size:        843     248     702    1496     349

  Fragments arranged by size: 

                1496     843     702     349     248
 
BsaWI w'CCGG_w

Cuts at:    473     827    1348    2026    2300    2447    2858     473 
   Size:        354     521     678     274     147     411    1253

  Fragments arranged by size: 

                1253     678     521     411     354     274     147
 
BseYI C'CCAG_C

Cuts at:    166     280     480    1063    1821    2398    2894     166 
   Size:        114     200     583     758     577     496     910

  Fragments arranged by size: 

                 910     758     583     577     496     200     114
 
BsiEI CG_ry'CG

Cuts at:    104    1180    1226    2434     104 
   Size:       1076      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1076      46
 
BsiHKAI G_wGCw'C

Cuts at:    200     652    1430    1620    2412     200 
   Size:        452     778     190     792    1426

  Fragments arranged by size: 

                1426     792     778     452     190
 
Bsp1286I G_dGCh'C

Cuts at:    200     279     652    1263    1356    1430    1620    1682    2412 
   Size:         79     373     611      93      74     190      62     730

Cuts at:   2412     200 
   Size:       1426

  Fragments arranged by size: 

                1426     730     611     373     190      93      79      74
                  62
 
BspEI T'CCGG_A

Cuts at:   2858    2858 
   Size:       3638
 
BspHI T'CATG_A

Cuts at:   2814    2814 
   Size:       3638
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    688     902    1204    1585     688 
   Size:        214     302     381    2741

  Fragments arranged by size: 

                2741     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1930    1984    3468    3633      50 
   Size:       1880      54    1484     165      55

  Fragments arranged by size: 

                1880    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1550    1550 
   Size:       3638
 
BsrFI r'CCGG_y

Cuts at:   1636    1817    2026    1636 
   Size:        181     209    3248

  Fragments arranged by size: 

                3248     209     181
 
BssSI C'ACGA_G

Cuts at:   1909    2267    1909 
   Size:        358    3280
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1015    1015 
   Size:       3638
 
BstBI TT'CG_AA

Cuts at:   1999    1999 
   Size:       3638
 
BstYI r'GATC_y

Cuts at:      1     134     476     663    1130    1153    1488    1734    2746 
   Size:        133     342     187     467      23     335     246    1012

Cuts at:   2746    2832    2844    3190       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     467     449     346     342     335     246     187
                 133      86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3638
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:     99    1588    1769    1932      99 
   Size:       1489     181     163    1805

  Fragments arranged by size: 

                1805    1489     181     163
 
BtsI GCAGTG_nn'

Cuts at:    881     881 
   Size:       3638
 
DraI TTT'AAA

Cuts at:    637     637 
   Size:       3638
 
DrdI GACnn_nn'nnGTC

Cuts at:   1344    2202    1344 
   Size:        858    2780
 
EaeI y'GGCC_r

Cuts at:    256    1223    1397    1788    1815    3160     256 
   Size:        967     174     391      27    1345     734

  Fragments arranged by size: 

                1345     967     734     391     174      27
 
EagI C'GGCC_G

Cuts at:   1223    1223 
   Size:       3638
 
EarI CTCTTCn'nnn_

Cuts at:    416     593    1661    1871     416 
   Size:        177    1068     210    2183

  Fragments arranged by size: 

                2183    1068     210     177
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    127    1846    2156    2302    3448     127 
   Size:       1719     310     146    1146     317

  Fragments arranged by size: 

                1719    1146     317     310     146
 
EcoICRI GAG'CTC

Cuts at:    650     650 
   Size:       3638
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    435    1463    1895    2642    2910     435 
   Size:       1028     432     747     268    1163

  Fragments arranged by size: 

                1163    1028     747     432     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:    319     319 
   Size:       3638
 
EcoO109I rG'GnC_Cy

Cuts at:    531     777     531 
   Size:        246    3392
 
EcoRI G'AATT_C

Cuts at:    642     642 
   Size:       3638
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1657    1657 
   Size:       3638
 
FspI TGC'GCA

Cuts at:   1419    1419 
   Size:       3638
 
HaeII r_GCGC'y

Cuts at:    429    1072    1320    2342    3321     429 
   Size:        643     248    1022     979     746

  Fragments arranged by size: 

                1022     979     746     643     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    316     348    1480    1510    1512    1542     316 
   Size:         32    1132      30       2      30    2412

  Fragments arranged by size: 

                2412    1132      32      30      30       2
 
HincII GTy'rAC

Cuts at:    339     677     339 
   Size:        338    3300
 
KasI G'GCGC_C

Cuts at:   1068    1316    1068 
   Size:        248    3390
 
KpnI G_GTAC'C

Cuts at:    658     658 
   Size:       3638
 
MluI A'CGCG_T

Cuts at:   3071    3071 
   Size:       3638
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    185    2309    2493     185 
   Size:       2124     184    1330

  Fragments arranged by size: 

                2124    1330     184
 
MscI TGG'CCA

Cuts at:   1399    1399 
   Size:       3638
 
MslI CAynn'nnrTG

Cuts at:   1627    1754    1627 
   Size:        127    3511
 
MspA1I CmG'CkG

Cuts at:    284     484     749    1063    1423    2436    2681     284 
   Size:        200     265     314     360    1013     245    1241

  Fragments arranged by size: 

                1241    1013     360     314     265     245     200
 
NaeI GCC'GGC

Cuts at:   1819    1819 
   Size:       3638
 
NarI GG'CG_CC

Cuts at:   1069    1317    1069 
   Size:        248    3390
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3638
 
NgoMIV G'CCGG_C

Cuts at:   1817    1817 
   Size:       3638
 
NspI r_CATG'y

Cuts at:    691    1191    2098     691 
   Size:        500     907    2231

  Fragments arranged by size: 

                2231     907     500
 
PciI A'CATG_T

Cuts at:   2094    2094 
   Size:       3638
 
PmeI GTTT'AAAC

Cuts at:    637     637 
   Size:       3638
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2777    2809    2777 
   Size:         32    3606
 
PpuMI rG'GwC_Cy

Cuts at:    531     531 
   Size:       3638
 
PstI C_TGCA'G

Cuts at:    685    1370     685 
   Size:        685    2953
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3638
 
PvuII CAG'CTG

Cuts at:    284     484    1063    1423     284 
   Size:        200     579     360    2499

  Fragments arranged by size: 

                2499     579     360     200
 
RsrII CG'GwC_CG

Cuts at:   1833    1833 
   Size:       3638
 
SacI G_AGCT'C

Cuts at:    652     652 
   Size:       3638
 
SalI G'TCGA_C

Cuts at:    675     675 
   Size:       3638
 
SapI GCTCTTCn'nnn_

Cuts at:    416    1661    1871     416 
   Size:       1245     210    2183

  Fragments arranged by size: 

                2183    1245     210
 
SbfI CC_TGCA'GG

Cuts at:    685     685 
   Size:       3638
 
ScaI AGT'ACT

Cuts at:   3155    3155 
   Size:       3638
 
SfcI C'TryA_G

Cuts at:     33     681    1366    2359    2550      33 
   Size:        648     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     648     191
 
SfoI GGC'GCC

Cuts at:   1070    1318    1070 
   Size:        248    3390
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3638
 
SmaI CCC'GGG

Cuts at:    660     660 
   Size:       3638
 
SmlI C'TyrA_G

Cuts at:    793    1708    2200    2462     793 
   Size:        915     492     262    1969

  Fragments arranged by size: 

                1969     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3638
 
SphI G_CATG'C

Cuts at:    691    1191     691 
   Size:        500    3138
 
StyI C'CwwG_G

Cuts at:    107     550     772    1126     107 
   Size:        443     222     354    2619

  Fragments arranged by size: 

                2619     443     354     222
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    294    1221    1354    1813    2022     294 
   Size:        927     133     459     209    1910

  Fragments arranged by size: 

                1910     927     459     209     133
 
TatI w'GTAC_w

Cuts at:   3153    3153 
   Size:       3638
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    251     283    3155    3187     251 
   Size:         32    2872      32     702

  Fragments arranged by size: 

                2872     702      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1435    1435 
   Size:       3638
 
XbaI T'CTAG_A

Cuts at:    669     669 
   Size:       3638
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    436    1133     436 
   Size:        697    2941
 
XmaI C'CCGG_G

Cuts at:    658     658 
   Size:       3638
 
XmnI GAAnn'nnTTC

Cuts at:    355     355 
   Size:       3638
 
ZraI GAC'GTC

Cuts at:    227     227 
   Size:       3638
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI
     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PciI     PmeI     PpiI
    PpuMI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI     BglI
    BmgBI     BmtI    BsaXI    BseRI     BsgI    BsiWI     BsmI    BsmBI
    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI
   DraIII    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI
    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI
     XhoI
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