Restriction map for
pF1KE0396
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# NO CUT ENZYMES ( 66 enzymes) #
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AatII Acc65I AccI AclI AflII
AgeI Aor51HI AscI AseI AsnI
Asp718I AviII BamHI BciVI BfrI
BglII BsiWI Bsp1407I BspDI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstXI BstYI BstZ17I ClaI DraI
Eco47III EcoRV EcoT22I FseI FspI
HindIII KpnI MflI MluI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI SalI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI XhoI
XhoII
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 2228 TCCGGA T^CCGGA
Alw44I 2576 GTGCAC G^TGCAC
AlwI 1167 GGATC GGATC(4/5)
ApaLI 2576 GTGCAC G^TGCAC
AvrII 2991 CCTAGG C^CTAGG
BbrPI 2276 CACGTG CAC^GTG
BcgI 2488 CGAGGCATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2772 TGATCA T^GATCA
BglI 2026 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2991 CCTAGG C^CTAGG
BsaBI 1938 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2228 TCCGGA [AT]^CCGG[AT]
BseAI 2228 TCCGGA T^CCGGA
BsmBI 730 GAGACG CGTCTC(1/5)
BsmI 1251 GAATGC GAATGC(1/-1)
BspEI 2228 TCCGGA T^CCGGA
BspHI 1380 TCATGA T^CATGA
BspLU11I 603 ACATGT A^CATGT
BstEII 1266 GGTGACC G^GT[ACGT]ACC
BstPI 1266 GGTGACC G^GT[ACGT]ACC
CpoI 1397 CGGACCG CG^G[AT]CCG
DrdI 681 GACCACGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1280 CGGCCG C^GGCCG
EclXI 1280 CGGCCG C^GGCCG
Eco52I 1280 CGGCCG C^GGCCG
EcoO65I 1266 GGTGACC G^GT[ACGT]ACC
EcoRI 1798 GAATTC G^AATTC
FbaI 2772 TGATCA T^GATCA
HgaI 732 GACGC GACGC(5/10)
HpaI 1125 GTTAAC GTT^AAC
KspI 2654 CCGCGG CCGC^GG
MamI 1938 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2228 TCCGGA T^CCGGA
MseI 1126 TTAA T^TAA
NaeI 998 GCCGGC GCC^GGC
NcoI 2585 CCATGG C^CATGG
NgoMIV 998 GCCGGC G^CCGGC
PmaCI 2276 CACGTG CAC^GTG
PmlI 2276 CACGTG CAC^GTG
RcaI 1380 TCATGA T^CATGA
RsrII 1397 CGGACCG CG^G[AT]CCG
SacI 838 GAGCTC GAGCT^C
SacII 2654 CCGCGG CCGC^GG
SbfI 3009 CCTGCAGG CCTGCA^GG
Sse8387I 3009 CCTGCAGG CCTGCA^GG
Tru9I 1126 TTAA T^TAA
XbaI 3072 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 25 enzymes) #
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AflIII 603 ACATGT A^C[GA][CT]GT
1688 ACGTGT
AhdI 337 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1269 GACCAGCCGTC
AocI 1299 CCTCAGG CC^T[ACGT]AGG
1365 CCTGAGG
ApaI 2322 GGGCCC GGGCC^C
2744 GGGCCC
Asp700I 1798 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3060 GAACACTTTC
AspEI 337 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1269 GACCAGCCGTC
BbvCI 1427 CCTCAGC CCTCAGC(-5/-2)
675 GCTGAGG
BlpI 2590 GCTAAGC GC^T[ACGT]AGC
2793 GCTCAGC
Bpu1102I 2590 GCTAAGC GC^T[ACGT]AGC
2793 GCTCAGC
BsaAI 1687 TACGTG [CT]AC^GT[GA]
2276 CACGTG
BsiEI 1190 CGACCG CG[GA][CT]^CG
1280 CGGCCG
BsrBI 472 GAGCGG CCGCTC(-3/-3)
2254 GAGCGG
BsrFI 410 ACCGGC [GA]^CCGG[CT]
998 GCCGGC
Bsu36I 1299 CCTCAGG CC^T[ACGT]AGG
1365 CCTGAGG
CelII 2590 GCTAAGC GC^T[ACGT]AGC
2793 GCTCAGC
Cfr10I 410 ACCGGC [GA]^CCGG[CT]
998 GCCGGC
Eam1105I 337 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1269 GACCAGCCGTC
Eco81I 1299 CCTCAGG CC^T[ACGT]AGG
1365 CCTGAGG
EcoNI 1227 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3006 CCTCCTGCAGG
MfeI 1890 CAATTG C^AATTG
2530 CAATTG
MunI 1890 CAATTG C^AATTG
2530 CAATTG
StuI 1949 AGGCCT AGG^CCT
2111 AGGCCT
TfiI 1548 GATTC G^A[AT]TC
2714 GATTC
XcmI 254 CCAGGCGCCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
821 CCACCCTGGGCCTGG
XmnI 1798 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3060 GAACACTTTC
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# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwNI ApoI AspHI AspI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI SphI StyI
TaqI TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XmaCI XmaI