Restriction map for
pF1KE0311
(Circular) (Six-base) MAPSORT of: pF1KE0311.seq Check: 2459 from: 1 to: 6908
pF1KE0311 6908 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2415 2415
Size: 6908
AatII G_ACGT'C
Cuts at: 1799 3337 1799
Size: 1538 5370
AccI GT'mk_AC
Cuts at: 3427 3946 3427
Size: 519 6389
Acc65I G'GTAC_C
Cuts at: 3924 3924
Size: 6908
AclI AA'CG_TT
Cuts at: 6748 6748
Size: 6908
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 662 2300 3809 4733 5165 5912 6180 662
Size: 1638 1509 924 432 747 268 1390
Fragments arranged by size:
1638 1509 1390 924 747 432 268
AflIII A'CryG_T
Cuts at: 1991 2901 4260 5364 6341 1991
Size: 910 1359 1104 977 2558
Fragments arranged by size:
2558 1359 1104 977 910
AgeI A'CCGG_T
Cuts at: 5296 5296
Size: 6908
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 577 577
Size: 6908
AlwNI CAG_nnn'CTG
Cuts at: 3202 4213 5780 3202
Size: 1011 1567 4330
Fragments arranged by size:
4330 1567 1011
ApaLI G'TGCA_C
Cuts at: 5678 5678
Size: 6908
ApoI r'AATT_y
Cuts at: 179 318 375 1642 1660 1677 1807 1845 2689
Size: 139 57 1267 18 17 130 38 844
Cuts at: 2689 2727 3032 3630 3700 3912 6261 6517 179
Size: 38 305 598 70 212 2349 256 570
Fragments arranged by size:
2349 1267 844 598 570 305 256 212
139 130 70 57 38 38 18 17
AseI AT'TA_AT
Cuts at: 20 1934 20
Size: 1914 4994
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6908
AvaI C'yCGr_G
Cuts at: 3928 3928
Size: 6908
BamHI G'GATC_C
Cuts at: 3933 3933
Size: 6908
BanI G'GyrC_C
Cuts at: 3924 4338 4586 4621 3924
Size: 414 248 35 6211
Fragments arranged by size:
6211 414 248 35
BanII G_rGCy'C
Cuts at: 3922 4952 3922
Size: 1030 5878
BbeI G_GCGC'C
Cuts at: 4342 4590 4342
Size: 248 6660
BbsI GAAGACnn'nnnn_
Cuts at: 559 1969 2269 2390 2851 3361 559
Size: 1410 300 121 461 510 4106
Fragments arranged by size:
4106 1410 510 461 300 121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1215 1249 1215
Size: 34 6874
BciVI GTATCCnnnnn_n'
Cuts at: 4806 5567 6908 4806
Size: 761 1341 4806
Fragments arranged by size:
4806 1341 761
BclI T'GATC_A
Cuts at: 3027 4428 3027
Size: 1401 5507
BglII A'GATC_T
Cuts at: 1411 2925 3270 3517 3816 4423 6460 1411
Size: 1514 345 247 299 607 2037 1859
Fragments arranged by size:
2037 1859 1514 607 345 299 247
BlpI GC'TnA_GC
Cuts at: 2795 4020 2795
Size: 1225 5683
Bme1580I G_kGCm'C
Cuts at: 118 4533 4626 5682 118
Size: 4415 93 1056 1344
Fragments arranged by size:
4415 1344 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 112 867 3211 4120 4201 4536 6680 112
Size: 755 2344 909 81 335 2144 340
Fragments arranged by size:
2344 2144 909 755 340 335 81
BmtI G_CTAG'C
Cuts at: 527 527
Size: 6908
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5275 5275
Size: 6908
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3359 3359
Size: 6908
Bpu10I CC'TnA_GC
Cuts at: 293 1502 2242 2824 293
Size: 1209 740 582 4377
Fragments arranged by size:
4377 1209 740 582
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4084 4963 5455 5753 4084
Size: 879 492 298 5239
Fragments arranged by size:
5239 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6612 6612
Size: 6908
BsaAI yAC'GTr
Cuts at: 4261 4261
Size: 6908
BsaBI GATnn'nnATC
Cuts at: 6 3203 4422 6459 6
Size: 3197 1219 2037 455
Fragments arranged by size:
3197 2037 1219 455
BsaHI Gr'CG_yC
Cuts at: 1796 3334 4339 4587 5289 6785 1796
Size: 1538 1005 248 702 1496 1919
Fragments arranged by size:
1919 1538 1496 1005 702 248
BsaWI w'CCGG_w
Cuts at: 1958 3713 4097 4618 5296 5570 5717 6128 1958
Size: 1755 384 521 678 274 147 411 2738
Fragments arranged by size:
2738 1755 678 521 411 384 274 147
BseYI C'CCAG_C
Cuts at: 2115 3651 4333 5091 5668 6164 2115
Size: 1536 682 758 577 496 2859
Fragments arranged by size:
2859 1536 758 682 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1460 1460
Size: 6908
BsiEI CG_ry'CG
Cuts at: 104 4450 4496 5704 104
Size: 4346 46 1208 1308
Fragments arranged by size:
4346 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 3649 3922 4700 4890 5682 3649
Size: 273 778 190 792 4875
Fragments arranged by size:
4875 792 778 273 190
BsmI GAATG_Cn'
Cuts at: 2971 2971
Size: 6908
Bsp1286I G_dGCh'C
Cuts at: 118 3649 3922 4533 4626 4700 4890 4952 5682
Size: 3531 273 611 93 74 190 62 730
Cuts at: 5682 118
Size: 1344
Fragments arranged by size:
3531 1344 730 611 273 190 93 74
62
BspEI T'CCGG_A
Cuts at: 6128 6128
Size: 6908
BspHI T'CATG_A
Cuts at: 446 6084 446
Size: 5638 1270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2415 2502 3958 4172 4474 4855 2415
Size: 87 1456 214 302 381 4468
Fragments arranged by size:
4468 1456 381 302 214 87
BsrBI CCG'CTC
Cuts at: 50 5200 5254 6738 6903 50
Size: 5150 54 1484 165 55
Fragments arranged by size:
5150 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4820 4820
Size: 6908
BsrFI r'CCGG_y
Cuts at: 4906 5087 5296 4906
Size: 181 209 6518
Fragments arranged by size:
6518 209 181
BsrGI T'GTAC_A
Cuts at: 2604 2604
Size: 6908
BssSI C'ACGA_G
Cuts at: 5179 5537 5179
Size: 358 6550
BstAPI GCAn_nnn'nTGC
Cuts at: 4285 4285
Size: 6908
BstBI TT'CG_AA
Cuts at: 5269 5269
Size: 6908
BstXI CCAn_nnnn'nTGG
Cuts at: 880 880
Size: 6908
BstYI r'GATC_y
Cuts at: 1 1089 1411 1446 2925 3270 3366 3517 3816
Size: 1088 322 35 1479 345 96 151 299
Cuts at: 3816 3933 4400 4423 4758 5004 6016 6102 6114
Size: 117 467 23 335 246 1012 86 12
Cuts at: 6114 6460 1
Size: 346 449
Fragments arranged by size:
1479 1088 1012 467 449 346 345 335
322 299 246 151 117 96 86 35
23 12
Bsu36I CC'TnA_GG
Cuts at: 147 147
Size: 6908
BtgI C'CryG_G
Cuts at: 107 107
Size: 6908
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4858 5039 5202 4858
Size: 181 163 6564
Fragments arranged by size:
6564 181 163
BtsI GCAGTG_nn'
Cuts at: 637 3788 4151 637
Size: 3151 363 3394
Fragments arranged by size:
3394 3151 363
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2409 2444 2409
Size: 35 6873
DraI TTT'AAA
Cuts at: 269 1359 2937 3176 3705 3870 3907 269
Size: 1090 1578 239 529 165 37 3270
Fragments arranged by size:
3270 1578 1090 529 239 165 37
DrdI GACnn_nn'nnGTC
Cuts at: 3339 4614 5472 3339
Size: 1275 858 4775
Fragments arranged by size:
4775 1275 858
EaeI y'GGCC_r
Cuts at: 882 2478 4493 4667 5058 5085 6430 882
Size: 1596 2015 174 391 27 1345 1360
Fragments arranged by size:
2015 1596 1360 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4493 4493
Size: 6908
EarI CTCTTCn'nnn_
Cuts at: 681 3894 4931 5141 681
Size: 3213 1037 210 2448
Fragments arranged by size:
3213 2448 1037 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5116 5426 5572 6718 5116
Size: 310 146 1146 5306
Fragments arranged by size:
5306 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3920 3920
Size: 6908
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 662 2300 3359 3809 4733 5165 5912 6180 662
Size: 1638 1059 450 924 432 747 268 1390
Fragments arranged by size:
1638 1390 1059 924 747 450 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1862 2159 4047 1862
Size: 297 1888 4723
Fragments arranged by size:
4723 1888 297
EcoRI G'AATT_C
Cuts at: 318 1677 3912 318
Size: 1359 2235 3314
Fragments arranged by size:
3314 2235 1359
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1721 3513 4927 1721
Size: 1792 1414 3702
Fragments arranged by size:
3702 1792 1414
FspI TGC'GCA
Cuts at: 4689 4689
Size: 6908
HaeII r_GCGC'y
Cuts at: 4342 4590 5612 6591 4342
Size: 248 1022 979 4659
Fragments arranged by size:
4659 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 658 690 886 918 1029 1061 1306 1338 1403
Size: 32 196 32 111 32 245 32 65
Cuts at: 1403 1435 2773 2805 4750 4780 4782 4812 658
Size: 32 1338 32 1945 30 2 30 2754
Fragments arranged by size:
2754 1945 1338 245 196 111 65 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3003 3134 3711 3947 3003
Size: 131 577 236 5964
Fragments arranged by size:
5964 577 236 131
HindIII A'AGCT_T
Cuts at: 424 1703 1939 2239 424
Size: 1279 236 300 5093
Fragments arranged by size:
5093 1279 300 236
HpaI GTT'AAC
Cuts at: 3134 3134
Size: 6908
KasI G'GCGC_C
Cuts at: 4338 4586 4338
Size: 248 6660
KpnI G_GTAC'C
Cuts at: 3928 3928
Size: 6908
MfeI C'AATT_G
Cuts at: 1171 1171
Size: 6908
MluI A'CGCG_T
Cuts at: 6341 6341
Size: 6908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5579 5763 5579
Size: 184 6724
MscI TGG'CCA
Cuts at: 2480 4669 2480
Size: 2189 4719
MslI CAynn'nnrTG
Cuts at: 1052 1994 2197 2480 4897 5024 1052
Size: 942 203 283 2417 127 2936
Fragments arranged by size:
2936 2417 942 283 203 127
MspA1I CmG'CkG
Cuts at: 1476 1497 4019 4333 4693 5706 5951 1476
Size: 21 2522 314 360 1013 245 2433
Fragments arranged by size:
2522 2433 1013 360 314 245 21
NaeI GCC'GGC
Cuts at: 5089 5089
Size: 6908
NarI GG'CG_CC
Cuts at: 4339 4587 4339
Size: 248 6660
NcoI C'CATG_G
Cuts at: 107 107
Size: 6908
NgoMIV G'CCGG_C
Cuts at: 5087 5087
Size: 6908
NheI G'CTAG_C
Cuts at: 523 523
Size: 6908
NspI r_CATG'y
Cuts at: 1995 2905 3961 4461 5368 1995
Size: 910 1056 500 907 3535
Fragments arranged by size:
3535 1056 910 907 500
PciI A'CATG_T
Cuts at: 1991 2901 5364 1991
Size: 910 2463 3535
Fragments arranged by size:
3535 2463 910
PfoI T'CCnGG_A
Cuts at: 1421 3207 1421
Size: 1786 5122
PmeI GTTT'AAAC
Cuts at: 3907 3907
Size: 6908
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6047 6079 6047
Size: 32 6876
PpuMI rG'GwC_Cy
Cuts at: 1862 2159 1862
Size: 297 6611
PstI C_TGCA'G
Cuts at: 969 3955 4640 969
Size: 2986 685 3237
Fragments arranged by size:
3237 2986 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6908
PvuII CAG'CTG
Cuts at: 1476 1497 4333 4693 1476
Size: 21 2836 360 3691
Fragments arranged by size:
3691 2836 360 21
RsrII CG'GwC_CG
Cuts at: 5103 5103
Size: 6908
SacI G_AGCT'C
Cuts at: 3922 3922
Size: 6908
SalI G'TCGA_C
Cuts at: 3945 3945
Size: 6908
SanDI GG'GwC_CC
Cuts at: 1862 1862
Size: 6908
SapI GCTCTTCn'nnn_
Cuts at: 4931 5141 4931
Size: 210 6698
SbfI CC_TGCA'GG
Cuts at: 3955 3955
Size: 6908
ScaI AGT'ACT
Cuts at: 6425 6425
Size: 6908
SfcI C'TryA_G
Cuts at: 33 788 965 2154 3951 4636 5629 5820 33
Size: 755 177 1189 1797 685 993 191 1121
Fragments arranged by size:
1797 1189 1121 993 755 685 191 177
SfoI GGC'GCC
Cuts at: 4340 4588 4340
Size: 248 6660
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6908
SmaI CCC'GGG
Cuts at: 3930 3930
Size: 6908
SmlI C'TyrA_G
Cuts at: 4063 4978 5470 5732 4063
Size: 915 492 262 5239
Fragments arranged by size:
5239 915 492 262
SpeI A'CTAG_T
Cuts at: 67 3187 67
Size: 3120 3788
SphI G_CATG'C
Cuts at: 3961 4461 3961
Size: 500 6408
SspI AAT'ATT
Cuts at: 645 645
Size: 6908
StyI C'CwwG_G
Cuts at: 107 3638 4042 4396 107
Size: 3531 404 354 2619
Fragments arranged by size:
3531 2619 404 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2761 4491 4624 5083 5292 2761
Size: 1730 133 459 209 4377
Fragments arranged by size:
4377 1730 459 209 133
TatI w'GTAC_w
Cuts at: 1566 2604 3853 6423 1566
Size: 1038 1249 2570 2051
Fragments arranged by size:
2570 2051 1249 1038
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 918 950 6425 6457 918
Size: 32 5475 32 1369
Fragments arranged by size:
5475 1369 32 32
Tth111I GACn'n_nGTC
Cuts at: 4705 4705
Size: 6908
XbaI T'CTAG_A
Cuts at: 1708 3939 1708
Size: 2231 4677
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4403 4403
Size: 6908
XmaI C'CCGG_G
Cuts at: 3928 3928
Size: 6908
XmnI GAAnn'nnTTC
Cuts at: 1297 2561 1297
Size: 1264 5644
ZraI GAC'GTC
Cuts at: 1797 3335 1797
Size: 1538 5370
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PciI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BsaXI BseRI BsiWI
BsmBI BssHII BstEII BstZ17I ClaI DraIII EcoNI EcoRV
FseI FspAI NdeI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI