Restriction map for
pF1KE0291
##################################
# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccII AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BfrI BglI
BglII BlnI BsaAI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HaeII HhaI HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MfeI MluI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TaqI
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 488 GTCTAC GT^[AC][GT]AC
AccIII 570 TCCGGA T^CCGGA
AcyI 831 GACGCC G[GA]^CG[CT]C
AflIII 838 ACGTGT A^C[GA][CT]GT
AlwNI 1104 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 733 ATTAAT AT^TAAT
AsnI 733 ATTAAT AT^TAAT
AvaII 503 GGACC G^G[AT]CC
BalI 1184 TGGCCA TGG^CCA
BanI 817 GGCACC G^G[CT][GA]CC
BbsI 972 GAAGAC GAAGAC(2/6)
BfaI 811 CTAG C^TAG
BlpI 234 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 234 GCTAAGC GC^T[ACGT]AGC
BpuAI 972 GAAGAC GAAGAC(2/6)
BsaBI 321 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 831 GACGCC G[GA]^CG[CT]C
BsaI 535 GGTCTC GGTCTC(1/5)
BsaWI 570 TCCGGA [AT]^CCGG[AT]
BseAI 570 TCCGGA T^CCGGA
BseRI 1118 CTCCTC GAGGAG(10/8)
BsmBI 183 CGTCTC CGTCTC(1/5)
BspEI 570 TCCGGA T^CCGGA
BstAPI 890 GCAACTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 714 CCAAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 234 GCTAAGC GC^T[ACGT]AGC
DraII 129 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 331 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 248 CCATGG C^C[GA][CT]GG
EaeI 1184 TGGCCA [CT]^GGCC[GA]
EarI 657 GAAGAG CTCTTC(1/4)
EcoNI 281 CCTCTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 129 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 248 CCATGG C^C[AT][AT]GG
Hin1I 831 GACGCC G[GA]^CG[CT]C
Ksp632I 657 GAAGAG CTCTTC(1/4)
MaeI 811 CTAG C^TAG
MamI 321 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1184 TGGCCA TGG^CCA
MroI 570 TCCGGA T^CCGGA
MscI 1184 TGGCCA TGG^CCA
MslI 225 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 248 CCATGG C^CATGG
PleI 146 GAGTC GAGTC(4/5)
PshBI 733 ATTAAT AT^TAAT
SacI 529 GAGCTC GAGCT^C
SapI 657 GAAGAGC GCTCTTC(1/4)
SspI 736 AATATT AAT^ATT
StyI 248 CCATGG C^C[AT][AT]GG
Tsp45I 221 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 291 GTAC GT^AC
842 GTAC
AspHI 18 GAGCAC G[AT]GC[AT]^C
529 GAGCTC
BclI 323 TGATCA T^GATCA
1134 TGATCA
BcnI 506 CCCGG CC^[GC]GG
513 CCCGG
BsgI 177 CTGCAC GTGCAG(16/14)
593 GTGCAG
BsiHKAI 18 GAGCAC G[AT]GC[AT]^C
529 GAGCTC
BsmFI 631 GGGAC GGGAC(10/14)
710 GTCCC
BsrDI 779 CATTGC GCAATG(2/0)
626 GCAATG
BstYI 39 GGATCT [GA]^GATC[CT]
394 GGATCT
FbaI 323 TGATCA T^GATCA
1134 TGATCA
HgaI 831 GACGC GACGC(5/10)
239 GCGTC
MaeII 838 ACGT A^CGT
1140 ACGT
MaeIII 221 GTGAC ^GT[ACGT]AC
481 GTTAC
MflI 39 GGATCT [GA]^GATC[CT]
394 GGATCT
MspA1I 59 CAGCGG C[AC]G^C[GT]G
1126 CAGCGG
NciI 506 CCCGG CC^[GC]GG
513 CCCGG
PflMI 330 CCACAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
414 CCAAGTTATGG
RsaI 291 GTAC GT^AC
842 GTAC
SfaNI 365 GATGC GCATC(5/9)
602 GCATC
SfcI 918 CTGTAG C^T[GA][CT]AG
1214 CTGTAG
Tsp509I 947 AATT ^AATT
1024 AATT
Van91I 330 CCACAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
414 CCAAGTTATGG
XhoII 39 GGATCT [GA]^GATC[CT]
394 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AlwI BanII BbvI
BmyI BpmI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MboI MboII MnlI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI TfiI Tru9I
TseI TspRI