(Circular) (Six-base) MAPSORT of: pF1KE0291.seq Check: 2360 from: 1 to: 4334 pF1KE0291 4334 bp With 182 enzymes: SgfI * April 24, 2009 13:12 .. AccI GT'mk_AC Cuts at: 597 1372 597 Size: 775 3559 Acc65I G'GTAC_C Cuts at: 1350 1350 Size: 4334 AclI AA'CG_TT Cuts at: 4174 4174 Size: 4334 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 280 793 2159 2591 3338 3606 280 Size: 513 1366 432 747 268 1008 Fragments arranged by size: 1366 1008 747 513 432 268 AflIII A'CryG_T Cuts at: 946 1686 2790 3767 946 Size: 740 1104 977 1513 Fragments arranged by size: 1513 1104 977 740 AgeI A'CCGG_T Cuts at: 2722 2722 Size: 4334 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 521 521 Size: 4334 AlwNI CAG_nnn'CTG Cuts at: 1217 1639 3206 1217 Size: 422 1567 2345 Fragments arranged by size: 2345 1567 422 ApaLI G'TGCA_C Cuts at: 3104 3104 Size: 4334 ApoI r'AATT_y Cuts at: 1338 3687 3943 1338 Size: 2349 256 1729 Fragments arranged by size: 2349 1729 256 AseI AT'TA_AT Cuts at: 20 842 20 Size: 822 3512 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4334 AvaI C'yCGr_G Cuts at: 1354 1354 Size: 4334 BamHI G'GATC_C Cuts at: 1359 1359 Size: 4334 BanI G'GyrC_C Cuts at: 925 1350 1764 2012 2047 925 Size: 425 414 248 35 3212 Fragments arranged by size: 3212 425 414 248 35 BanII G_rGCy'C Cuts at: 268 324 365 452 641 686 1348 2378 268 Size: 56 41 87 189 45 662 1030 2224 Fragments arranged by size: 2224 1030 662 189 87 56 45 41 BbeI G_GCGC'C Cuts at: 1768 2016 1768 Size: 248 4086 BbsI GAAGACnn'nnnn_ Cuts at: 1087 1087 Size: 4334 BciVI GTATCCnnnnn_n' Cuts at: 2232 2993 4334 2232 Size: 761 1341 2232 Fragments arranged by size: 2232 1341 761 BclI T'GATC_A Cuts at: 431 1242 1854 431 Size: 811 612 2911 Fragments arranged by size: 2911 811 612 BglII A'GATC_T Cuts at: 1849 3886 1849 Size: 2037 2297 BlpI GC'TnA_GC Cuts at: 343 1446 343 Size: 1103 3231 Bme1580I G_kGCm'C Cuts at: 1959 2052 3108 1959 Size: 93 1056 3185 Fragments arranged by size: 3185 1056 93 BmgBI CAC'GTC Cuts at: 947 947 Size: 4334 BmrI ACTGGGnnnn_n' Cuts at: 446 542 1546 1627 1962 4106 446 Size: 96 1004 81 335 2144 674 Fragments arranged by size: 2144 1004 674 335 96 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2701 2701 Size: 4334 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 172 466 851 172 Size: 294 385 3655 Fragments arranged by size: 3655 385 294 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1510 2389 2881 3179 1510 Size: 879 492 298 2665 Fragments arranged by size: 2665 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 649 4038 649 Size: 3389 945 BsaAI yAC'GTr Cuts at: 1687 1687 Size: 4334 BsaBI GATnn'nnATC Cuts at: 6 433 1848 3885 6 Size: 427 1415 2037 455 Fragments arranged by size: 2037 1415 455 427 BsaHI Gr'CG_yC Cuts at: 940 1765 2013 2715 4211 940 Size: 825 248 702 1496 1063 Fragments arranged by size: 1496 1063 825 702 248 BsaWI w'CCGG_w Cuts at: 678 1523 2044 2722 2996 3143 3554 678 Size: 845 521 678 274 147 411 1458 Fragments arranged by size: 1458 845 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1217 1217 Size: 4334 BseYI C'CCAG_C Cuts at: 685 1759 2517 3094 3590 685 Size: 1074 758 577 496 1429 Fragments arranged by size: 1429 1074 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 270 722 270 Size: 452 3882 BsiEI CG_ry'CG Cuts at: 104 1876 1922 3130 104 Size: 1772 46 1208 1308 Fragments arranged by size: 1772 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 130 641 1348 2126 2316 3108 130 Size: 511 707 778 190 792 1356 Fragments arranged by size: 1356 792 778 707 511 190 BsmBI CGTCTCn'nnnn_ Cuts at: 297 297 Size: 4334 Bsp1286I G_dGCh'C Cuts at: 130 268 324 365 452 641 686 1348 1959 Size: 138 56 41 87 189 45 662 611 Cuts at: 1959 2052 2126 2316 2378 3108 130 Size: 93 74 190 62 730 1356 Fragments arranged by size: 1356 730 662 611 190 189 138 93 87 74 62 56 45 41 BspEI T'CCGG_A Cuts at: 678 3554 678 Size: 2876 1458 BspHI T'CATG_A Cuts at: 3510 3510 Size: 4334 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1384 1598 1900 2281 1384 Size: 214 302 381 3437 Fragments arranged by size: 3437 381 302 214 BsrBI CCG'CTC Cuts at: 50 2626 2680 4164 4329 50 Size: 2576 54 1484 165 55 Fragments arranged by size: 2576 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 741 886 2246 741 Size: 145 1360 2829 Fragments arranged by size: 2829 1360 145 BsrFI r'CCGG_y Cuts at: 2332 2513 2722 2332 Size: 181 209 3944 Fragments arranged by size: 3944 209 181 BssSI C'ACGA_G Cuts at: 2605 2963 2605 Size: 358 3976 BstAPI GCAn_nnn'nTGC Cuts at: 1004 1711 1004 Size: 707 3627 BstBI TT'CG_AA Cuts at: 2695 2695 Size: 4334 BstXI CCAn_nnnn'nTGG Cuts at: 829 829 Size: 4334 BstYI r'GATC_y Cuts at: 1 147 502 1359 1826 1849 2184 2430 3442 Size: 146 355 857 467 23 335 246 1012 Cuts at: 3442 3528 3540 3886 1 Size: 86 12 346 449 Fragments arranged by size: 1012 857 467 449 355 346 335 246 146 86 23 12 BtgI C'CryG_G Cuts at: 107 356 107 Size: 249 4085 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 195 2284 2465 2628 195 Size: 2089 181 163 1901 Fragments arranged by size: 2089 1901 181 163 BtsI GCAGTG_nn' Cuts at: 203 710 1577 203 Size: 507 867 2960 Fragments arranged by size: 2960 867 507 DraI TTT'AAA Cuts at: 1333 1333 Size: 4334 DraIII CAC_nnn'GTG Cuts at: 444 444 Size: 4334 DrdI GACnn_nn'nnGTC Cuts at: 2040 2898 2040 Size: 858 3476 EaeI y'GGCC_r Cuts at: 1292 1919 2093 2484 2511 3856 1292 Size: 627 174 391 27 1345 1770 Fragments arranged by size: 1770 1345 627 391 174 27 EagI C'GGCC_G Cuts at: 1919 1919 Size: 4334 EarI CTCTTCn'nnn_ Cuts at: 760 2357 2567 760 Size: 1597 210 2527 Fragments arranged by size: 2527 1597 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2542 2852 2998 4144 2542 Size: 310 146 1146 2732 Fragments arranged by size: 2732 1146 310 146 EcoICRI GAG'CTC Cuts at: 639 1346 639 Size: 707 3627 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 172 280 466 793 851 2159 2591 3338 3606 Size: 108 186 327 58 1308 432 747 268 Cuts at: 3606 172 Size: 900 Fragments arranged by size: 1308 900 747 432 327 268 186 108 58 EcoNI CCTnn'n_nnAGG Cuts at: 393 393 Size: 4334 EcoO109I rG'GnC_Cy Cuts at: 238 1473 238 Size: 1235 3099 EcoRI G'AATT_C Cuts at: 1338 1338 Size: 4334 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 928 2353 928 Size: 1425 2909 FspI TGC'GCA Cuts at: 2115 2115 Size: 4334 HaeII r_GCGC'y Cuts at: 1768 2016 3038 4017 1768 Size: 248 1022 979 2085 Fragments arranged by size: 2085 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 464 496 801 833 936 968 2176 2206 2208 Size: 32 305 32 103 32 1208 30 2 Cuts at: 2208 2238 464 Size: 30 2560 Fragments arranged by size: 2560 1208 305 103 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1373 1373 Size: 4334 KasI G'GCGC_C Cuts at: 1764 2012 1764 Size: 248 4086 KpnI G_GTAC'C Cuts at: 1354 1354 Size: 4334 MluI A'CGCG_T Cuts at: 3767 3767 Size: 4334 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 888 3005 3189 888 Size: 2117 184 2033 Fragments arranged by size: 2117 2033 184 MscI TGG'CCA Cuts at: 1294 2095 1294 Size: 801 3533 MslI CAynn'nnrTG Cuts at: 337 2323 2450 337 Size: 1986 127 2221 Fragments arranged by size: 2221 1986 127 MspA1I CmG'CkG Cuts at: 169 1236 1445 1759 2119 3132 3377 169 Size: 1067 209 314 360 1013 245 1126 Fragments arranged by size: 1126 1067 1013 360 314 245 209 NaeI GCC'GGC Cuts at: 2515 2515 Size: 4334 NarI GG'CG_CC Cuts at: 1765 2013 1765 Size: 248 4086 NcoI C'CATG_G Cuts at: 107 356 107 Size: 249 4085 NgoMIV G'CCGG_C Cuts at: 2513 2513 Size: 4334 NspI r_CATG'y Cuts at: 1387 1887 2794 1387 Size: 500 907 2927 Fragments arranged by size: 2927 907 500 PasI CC'CwG_GG Cuts at: 297 297 Size: 4334 PciI A'CATG_T Cuts at: 2790 2790 Size: 4334 PflMI CCAn_nnn'nTGG Cuts at: 444 528 444 Size: 84 4250 PfoI T'CCnGG_A Cuts at: 620 620 Size: 4334 PmeI GTTT'AAAC Cuts at: 1333 1333 Size: 4334 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 406 438 3473 3505 406 Size: 32 3035 32 1235 Fragments arranged by size: 3035 1235 32 32 PstI C_TGCA'G Cuts at: 1381 2066 1381 Size: 685 3649 PvuI CG_AT'CG Cuts at: 104 104 Size: 4334 PvuII CAG'CTG Cuts at: 1759 2119 1759 Size: 360 3974 RsrII CG'GwC_CG Cuts at: 2529 2529 Size: 4334 SacI G_AGCT'C Cuts at: 641 1348 641 Size: 707 3627 SalI G'TCGA_C Cuts at: 1371 1371 Size: 4334 SapI GCTCTTCn'nnn_ Cuts at: 760 2357 2567 760 Size: 1597 210 2527 Fragments arranged by size: 2527 1597 210 SbfI CC_TGCA'GG Cuts at: 1381 1381 Size: 4334 ScaI AGT'ACT Cuts at: 3851 3851 Size: 4334 SfcI C'TryA_G Cuts at: 33 1026 1322 1377 2062 3055 3246 33 Size: 993 296 55 685 993 191 1121 Fragments arranged by size: 1121 993 993 685 296 191 55 SfoI GGC'GCC Cuts at: 1766 2014 1766 Size: 248 4086 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4334 SmaI CCC'GGG Cuts at: 1356 1356 Size: 4334 SmlI C'TyrA_G Cuts at: 1489 2404 2896 3158 1489 Size: 915 492 262 2665 Fragments arranged by size: 2665 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4334 SphI G_CATG'C Cuts at: 1387 1887 1387 Size: 500 3834 SspI AAT'ATT Cuts at: 846 846 Size: 4334 StyI C'CwwG_G Cuts at: 107 356 1468 1822 107 Size: 249 1112 354 2619 Fragments arranged by size: 2619 1112 354 249 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 631 1269 1917 2050 2509 2718 631 Size: 638 648 133 459 209 2247 Fragments arranged by size: 2247 648 638 459 209 133 TatI w'GTAC_w Cuts at: 949 3849 949 Size: 2900 1434 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3851 3883 3851 Size: 32 4302 Tth111I GACn'n_nGTC Cuts at: 2131 2131 Size: 4334 XbaI T'CTAG_A Cuts at: 1365 1365 Size: 4334 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1829 1829 Size: 4334 XmaI C'CCGG_G Cuts at: 1354 1354 Size: 4334 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI Bpu10I BsaXI BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI