Restriction map for pF1KE0291
 (Linear) (Six-base) MAPSORT of: KIEE0291.seq  Check: 1160  from: 1  to: 1221



KIEE0291.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

AccI GT'mk_AC

Cuts at:      0     489    1221 
   Size:        489     732
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     172     685    1221 
   Size:        172     513     536

  Fragments arranged by size: 

                 536     513     172
 
AflIII A'CryG_T

Cuts at:      0     838    1221 
   Size:        838     383
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     413    1221 
   Size:        413     808
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1109    1221 
   Size:       1109     112
 
AseI AT'TA_AT

Cuts at:      0     734    1221 
   Size:        734     487
 
BanI G'GyrC_C

Cuts at:      0     817    1221 
   Size:        817     404
 
BanII G_rGCy'C

Cuts at:      0     160     216     257     344     533     578    1221 
   Size:        160      56      41      87     189      45     643

  Fragments arranged by size: 

                 643     189     160      87      56      45      41
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     979    1221 
   Size:        979     242
 
BclI T'GATC_A

Cuts at:      0     323    1134    1221 
   Size:        323     811      87

  Fragments arranged by size: 

                 811     323      87
 
BlpI GC'TnA_GC

Cuts at:      0     235    1221 
   Size:        235     986
 
BmgBI CAC'GTC

Cuts at:      0     839    1221 
   Size:        839     382
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     338     434    1221 
   Size:        338      96     787

  Fragments arranged by size: 

                 787     338      96
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     358     743    1221 
   Size:         64     294     385     478

  Fragments arranged by size: 

                 478     385     294      64
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     541    1221 
   Size:        541     680
 
BsaBI GATnn'nnATC

Cuts at:      0     325    1221 
   Size:        325     896
 
BsaHI Gr'CG_yC

Cuts at:      0     832    1221 
   Size:        832     389
 
BsaWI w'CCGG_w

Cuts at:      0     570    1221 
   Size:        570     651
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1109    1221 
   Size:       1109     112
 
BseYI C'CCAG_C

Cuts at:      0     577    1221 
   Size:        577     644
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     162     614    1221 
   Size:        162     452     607

  Fragments arranged by size: 

                 607     452     162
 
BsiHKAI G_wGCw'C

Cuts at:      0      22     533    1221 
   Size:         22     511     688

  Fragments arranged by size: 

                 688     511      22
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     189    1221 
   Size:        189    1032
 
Bsp1286I G_dGCh'C

Cuts at:      0      22     160     216     257     344     533     578    1221 
   Size:         22     138      56      41      87     189      45     643

  Fragments arranged by size: 

                 643     189     138      87      56      45      41      22
            
 
BspEI T'CCGG_A

Cuts at:      0     570    1221 
   Size:        570     651
 
BsrDI GCAATG_nn'

Cuts at:      0     633     778    1221 
   Size:        633     145     443

  Fragments arranged by size: 

                 633     443     145
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     896    1221 
   Size:        896     325
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     721    1221 
   Size:        721     500
 
BstYI r'GATC_y

Cuts at:      0      39     394    1221 
   Size:         39     355     827

  Fragments arranged by size: 

                 827     355      39
 
BtgI C'CryG_G

Cuts at:      0     248    1221 
   Size:        248     973
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      87    1221 
   Size:         87    1134
 
BtsI GCAGTG_nn'

Cuts at:      0      95     602    1221 
   Size:         95     507     619

  Fragments arranged by size: 

                 619     507      95
 
DraIII CAC_nnn'GTG

Cuts at:      0     336    1221 
   Size:        336     885
 
EaeI y'GGCC_r

Cuts at:      0    1184    1221 
   Size:       1184      37
 
EarI CTCTTCn'nnn_

Cuts at:      0     652    1221 
   Size:        652     569
 
EcoICRI GAG'CTC

Cuts at:      0     531    1221 
   Size:        531     690
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     172     358     685     743    1221 
   Size:         64     108     186     327      58     478

  Fragments arranged by size: 

                 478     327     186     108      64      58
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     285    1221 
   Size:        285     936
 
EcoO109I rG'GnC_Cy

Cuts at:      0     130    1221 
   Size:        130    1091
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     820    1221 
   Size:        820     401
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     356     388     693     725     828     860    1221 
   Size:        356      32     305      32     103      32     361

  Fragments arranged by size: 

                 361     356     305     103      32      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     780    1221 
   Size:        780     441
 
MscI TGG'CCA

Cuts at:      0    1186    1221 
   Size:       1186      35
 
MslI CAynn'nnrTG

Cuts at:      0     229    1221 
   Size:        229     992
 
MspA1I CmG'CkG

Cuts at:      0      61    1128    1221 
   Size:         61    1067      93

  Fragments arranged by size: 

                1067      93      61
 
NcoI C'CATG_G

Cuts at:      0     248    1221 
   Size:        248     973
 
PasI CC'CwG_GG

Cuts at:      0     189    1221 
   Size:        189    1032
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     336     420    1221 
   Size:        336      84     801

  Fragments arranged by size: 

                 801     336      84
 
PfoI T'CCnGG_A

Cuts at:      0     512    1221 
   Size:        512     709
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     298     330    1221 
   Size:        298      32     891

  Fragments arranged by size: 

                 891     298      32
 
SacI G_AGCT'C

Cuts at:      0     533    1221 
   Size:        533     688
 
SapI GCTCTTCn'nnn_

Cuts at:      0     652    1221 
   Size:        652     569
 
SfcI C'TryA_G

Cuts at:      0     918    1214    1221 
   Size:        918     296       7

  Fragments arranged by size: 

                 918     296       7
 
SspI AAT'ATT

Cuts at:      0     738    1221 
   Size:        738     483
 
StyI C'CwwG_G

Cuts at:      0     248    1221 
   Size:        248     973
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     523    1161    1221 
   Size:        523     638      60

  Fragments arranged by size: 

                 638     523      60
 
TatI w'GTAC_w

Cuts at:      0     841    1221 
   Size:        841     380
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AlfI    AlwNI     AseI     BanI    BanII
     BbsI     BclI     BlpI    BmgBI     BmrI     BpmI     BsaI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I
    BspEI    BsrDI   BstAPI    BstXI    BstYI     BtgI    BtgZI     BtsI
   DraIII     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
    Hin4I     MmeI     MscI     MslI   MspA1I     NcoI     PasI    PflMI
     PfoI     PpiI     SacI     SapI     SfcI     SspI     StyI    TaqII
     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AloI     ApaI    ApaLI     ApoI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI    BfrBI
     BglI    BglII Bme1580I     BmtI     BplI   Bpu10I    BpuEI    BsaAI
    BsaXI    BsiEI    BsiWI     BsmI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I     ClaI
    CspCI     DraI     DrdI     EagI     EciI    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI     PmeI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com