Restriction map for pF1KE0289
##################################
# NO CUT ENZYMES (  164 enzymes) #
##################################

AatII       AccI        AccII       AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       Aor51HI     ApaI        ApaLI       
ApoI        AscI        AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BanII       
BbeI        BbrPI       BbsI        BbvCI       BbvI        
BcgI        BciVI       BcnI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstZ17I     CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        Fnu4HI      FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     ItaI        KasI        Ksp632I     KspI        
MamI        MluI        MvnI        MwoI        NaeI        
NarI        NciI        NdeI        NgoMIV      NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
TseI        Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

Acc65I        757  GGTACC               G^GTACC
AccIII        607  TCCGGA               T^CCGGA
AciI          363  CCGC                 CCGC(-3/-1)
AocI          675  CCTAAGG              CC^T[ACGT]AGG
AseI          468  ATTAAT               AT^TAAT
AsnI          468  ATTAAT               AT^TAAT
Asp700I       921  GAAACATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       757  GGTACC               G^GTACC
BamHI         680  GGATCC               G^GATCC
BanI          757  GGTACC               G^G[CT][GA]CC
BclI          641  TGATCA               T^GATCA
BsaJI         620  CCATGG               C^C[ACGT][ACGT]GG
BsaWI         607  TCCGGA               [AT]^CCGG[AT]
BseAI         607  TCCGGA               T^CCGGA
BseRI         489  GAGGAG               GAGGAG(10/8)
BsmAI         492  GAGAC                GTCTC(1/5)
BsmFI         383  GTCCC                GGGAC(10/14)
BspEI         607  TCCGGA               T^CCGGA
BsrI          792  CCAGT                ACTGG(1/-1)
BstF5I        207  GGATG                GGATG(2/0)
BstXI         236  CCAAAATGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         680  GGATCC               [GA]^GATC[CT]
Bsu36I        675  CCTAAGG              CC^T[ACGT]AGG
Cfr13I        908  GGGCC                G^G[ACGT]CC
DsaI          620  CCATGG               C^C[GA][CT]GG
Eco81I        675  CCTAAGG              CC^T[ACGT]AGG
EcoT14I       620  CCATGG               C^C[AT][AT]GG
FbaI          641  TGATCA               T^GATCA
FokI          207  GGATG                GGATG(9/13)
HapII         608  CCGG                 C^CGG
HpaII         608  CCGG                 C^CGG
KpnI          757  GGTACC               GGTAC^C
MaeII         887  ACGT                 A^CGT
MfeI          227  CAATTG               C^AATTG
MflI          680  GGATCC               [GA]^GATC[CT]
MroI          607  TCCGGA               T^CCGGA
MspA1I        189  CAGCTG               C[AC]G^C[GT]G
MspI          608  CCGG                 C^CGG
MunI          227  CAATTG               C^AATTG
NcoI          620  CCATGG               C^CATGG
NheI          670  GCTAGC               G^CTAGC
PshBI         468  ATTAAT               AT^TAAT
PvuII         189  CAGCTG               CAG^CTG
Sau96I        908  GGGCC                G^G[ACGT]CC
SfcI          398  CTGTAG               C^T[GA][CT]AG
SmlI          486  CTTGAG               C^T[CT][GA]AG
StyI          620  CCATGG               C^C[AT][AT]GG
XhoII         680  GGATCC               [GA]^GATC[CT]
XmnI          921  GAAACATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

BalI          371  TGGCCA               TGG^CCA
              623  TGGCCA              
BfaI          671  CTAG                 C^TAG
              838  CTAG                
BsrDI         615  CATTGC               GCAATG(2/0)
              660  CATTGC              
BssKI          93  CCTGG                ^CC[ACGT]GG
              255  CCTGG               
BstNI          93  CCTGG                CC^[AT]GG
              255  CCTGG               
EaeI          371  TGGCCA               [CT]^GGCC[GA]
              623  TGGCCA              
EcoNI         113  CCTTGTTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              500  CCTTTTGTAGG         
EcoRII         93  CCTGG                ^CC[AT]GG
              255  CCTGG               
HincII        460  GTTAAC               GT[CT]^[GA]AC
              597  GTTAAC              
HindII        460  GTTAAC               GT[CT]^[GA]AC
              597  GTTAAC              
HpaI          460  GTTAAC               GTT^AAC
              597  GTTAAC              
MaeI          671  CTAG                 C^TAG
              838  CTAG                
MboII         530  TCTTC                GAAGA(8/7)
              656  TCTTC               
MluNI         371  TGGCCA               TGG^CCA
              623  TGGCCA              
MscI          371  TGGCCA               TGG^CCA
              623  TGGCCA              
MvaI           93  CCTGG                CC^[AT]GG
              255  CCTGG               
NlaIV         680  GGATCC               GG[ACGT]^[ACGT]CC
              757  GGTACC              
PleI          381  GAGTC                GAGTC(4/5)
              611  GAGTC               
ScrFI          93  CCTGG                CC^[ACGT]GG
              255  CCTGG               
SfaNI         208  GATGC                GCATC(5/9)
              691  GCATC               
TfiI          123  GAATC                G^A[AT]TC
              843  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AfaI        AluI        AlwI        BsiYI       BslI        
Cac8I       DdeI        DpnII       HaeIII      HinfI       
HphI        MaeIII      MboI        MnlI        MseI        
MslI        NdeII       NlaIII      RsaI        Sau3AI      
Tru9I       Tsp45I      Tsp509I     TspRI       
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