Restriction map for pF1KE0279
 (Circular) (Six-base) MAPSORT of: pF1KE0279.seq  Check: 8054  from: 1  to: 3737



pF1KE0279  3737 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

AccI GT'mk_AC

Cuts at:    380     599     775     380 
   Size:        219     176    3342

  Fragments arranged by size: 

                3342     219     176
 
Acc65I G'GTAC_C

Cuts at:    753     753 
   Size:       3737
 
AclI AA'CG_TT

Cuts at:   3577    3577 
   Size:       3737
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1562    1994    2741    3009    1562 
   Size:        432     747     268    2290

  Fragments arranged by size: 

                2290     747     432     268
 
AflIII A'CryG_T

Cuts at:   1089    2193    3170    1089 
   Size:       1104     977    1656

  Fragments arranged by size: 

                1656    1104     977
 
AgeI A'CCGG_T

Cuts at:   2125    2125 
   Size:       3737
 
AhdI GACnn_n'nnGTC

Cuts at:    456     456 
   Size:       3737
 
AlwNI CAG_nnn'CTG

Cuts at:   1042    2609    1042 
   Size:       1567    2170
 
ApaLI G'TGCA_C

Cuts at:   2507    2507 
   Size:       3737
 
ApoI r'AATT_y

Cuts at:    325     741    3090    3346     325 
   Size:        416    2349     256     716

  Fragments arranged by size: 

                2349     716     416     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3737
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3737
 
AvaI C'yCGr_G

Cuts at:    757     757 
   Size:       3737
 
BamHI G'GATC_C

Cuts at:    762     762 
   Size:       3737
 
BanI G'GyrC_C

Cuts at:    703     753    1167    1415    1450     703 
   Size:         50     414     248      35    2990

  Fragments arranged by size: 

                2990     414     248      50      35
 
BanII G_rGCy'C

Cuts at:    751    1781     751 
   Size:       1030    2707
 
BbeI G_GCGC'C

Cuts at:   1171    1419    1171 
   Size:        248    3489
 
BbsI GAAGACnn'nnnn_

Cuts at:    254     254 
   Size:       3737
 
BciVI GTATCCnnnnn_n'

Cuts at:   1635    2396    3737    1635 
   Size:        761    1341    1635

  Fragments arranged by size: 

                1635    1341     761
 
BclI T'GATC_A

Cuts at:   1257    1257 
   Size:       3737
 
BglII A'GATC_T

Cuts at:    215    1252    3289     215 
   Size:       1037    2037     663

  Fragments arranged by size: 

                2037    1037     663
 
BlpI GC'TnA_GC

Cuts at:    849     849 
   Size:       3737
 
Bme1580I G_kGCm'C

Cuts at:    192    1362    1455    2511     192 
   Size:       1170      93    1056    1418

  Fragments arranged by size: 

                1418    1170    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    122     949    1030    1365    3509     122 
   Size:        827      81     335    2144     350

  Fragments arranged by size: 

                2144     827     350     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2104    2104 
   Size:       3737
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    525     913    1792    2284    2582     525 
   Size:        388     879     492     298    1680

  Fragments arranged by size: 

                1680     879     492     388     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    683    3441     683 
   Size:       2758     979
 
BsaAI yAC'GTr

Cuts at:    414    1090     414 
   Size:        676    3061
 
BsaBI GATnn'nnATC

Cuts at:      6    1251    3288       6 
   Size:       1245    2037     455

  Fragments arranged by size: 

                2037    1245     455
 
BsaHI Gr'CG_yC

Cuts at:   1168    1416    2118    3614    1168 
   Size:        248     702    1496    1291

  Fragments arranged by size: 

                1496    1291     702     248
 
BsaWI w'CCGG_w

Cuts at:    645     926    1447    2125    2399    2546    2957     645 
   Size:        281     521     678     274     147     411    1425

  Fragments arranged by size: 

                1425     678     521     411     281     274     147
 
BseYI C'CCAG_C

Cuts at:    313    1162    1920    2497    2993     313 
   Size:        849     758     577     496    1057

  Fragments arranged by size: 

                1057     849     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1279    1325    2533     104 
   Size:       1175      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1175      46
 
BsiHKAI G_wGCw'C

Cuts at:    413     751    1529    1719    2511     413 
   Size:        338     778     190     792    1639

  Fragments arranged by size: 

                1639     792     778     338     190
 
Bsp1286I G_dGCh'C

Cuts at:    192     413     751    1362    1455    1529    1719    1781    2511 
   Size:        221     338     611      93      74     190      62     730

Cuts at:   2511     192 
   Size:       1418

  Fragments arranged by size: 

                1418     730     611     338     221     190      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:    645    2957     645 
   Size:       2312    1425
 
BspHI T'CATG_A

Cuts at:   2913    2913 
   Size:       3737
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    787    1001    1303    1684     787 
   Size:        214     302     381    2840

  Fragments arranged by size: 

                2840     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2029    2083    3567    3732      50 
   Size:       1979      54    1484     165      55

  Fragments arranged by size: 

                1979    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    724    1649     724 
   Size:        925    2812
 
BsrFI r'CCGG_y

Cuts at:   1735    1916    2125    1735 
   Size:        181     209    3347

  Fragments arranged by size: 

                3347     209     181
 
BssSI C'ACGA_G

Cuts at:    475    2008    2366     475 
   Size:       1533     358    1846

  Fragments arranged by size: 

                1846    1533     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    351    1114     351 
   Size:        763    2974
 
BstBI TT'CG_AA

Cuts at:   2098    2098 
   Size:       3737
 
BstYI r'GATC_y

Cuts at:      1     215     762    1229    1252    1587    1833    2845    2931 
   Size:        214     547     467      23     335     246    1012      86

Cuts at:   2931    2943    3289       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     547     467     449     346     335     246     214
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3737
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1687    1868    2031    1687 
   Size:        181     163    3393

  Fragments arranged by size: 

                3393     181     163
 
BtsI GCAGTG_nn'

Cuts at:    980     980 
   Size:       3737
 
DraI TTT'AAA

Cuts at:    736     736 
   Size:       3737
 
DrdI GACnn_nn'nnGTC

Cuts at:   1443    2301    1443 
   Size:        858    2879
 
EaeI y'GGCC_r

Cuts at:    479    1322    1496    1887    1914    3259     479 
   Size:        843     174     391      27    1345     957

  Fragments arranged by size: 

                1345     957     843     391     174      27
 
EagI C'GGCC_G

Cuts at:   1322    1322 
   Size:       3737
 
EarI CTCTTCn'nnn_

Cuts at:   1760    1970    1760 
   Size:        210    3527
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1945    2255    2401    3547    1945 
   Size:        310     146    1146    2135

  Fragments arranged by size: 

                2135    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    749     749 
   Size:       3737
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1562    1994    2741    3009    1562 
   Size:        432     747     268    2290

  Fragments arranged by size: 

                2290     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    582     876     582 
   Size:        294    3443
 
EcoRI G'AATT_C

Cuts at:    325     741     325 
   Size:        416    3321
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    420    1756     420 
   Size:       1336    2401
 
FspI TGC'GCA

Cuts at:    355    1518     355 
   Size:       1163    2574
 
FspAI rTGC'GCAy

Cuts at:    355     355 
   Size:       3737
 
HaeII r_GCGC'y

Cuts at:    122     335    1171    1419    2441    3420     122 
   Size:        213     836     248    1022     979     439

  Fragments arranged by size: 

                1022     979     836     439     248     213
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    264     296     442     474    1579    1609    1611    1641     264 
   Size:         32     146      32    1105      30       2      30    2360

  Fragments arranged by size: 

                2360    1105     146      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    600     776     600 
   Size:        176    3561
 
KasI G'GCGC_C

Cuts at:   1167    1415    1167 
   Size:        248    3489
 
KpnI G_GTAC'C

Cuts at:    757     757 
   Size:       3737
 
MluI A'CGCG_T

Cuts at:   3170    3170 
   Size:       3737
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2408    2592    2408 
   Size:        184    3553
 
MscI TGG'CCA

Cuts at:    481    1498     481 
   Size:       1017    2720
 
MslI CAynn'nnrTG

Cuts at:   1726    1853    1726 
   Size:        127    3610
 
MspA1I CmG'CkG

Cuts at:    313     848    1162    1522    2535    2780     313 
   Size:        535     314     360    1013     245    1270

  Fragments arranged by size: 

                1270    1013     535     360     314     245
 
NaeI GCC'GGC

Cuts at:   1918    1918 
   Size:       3737
 
NarI GG'CG_CC

Cuts at:   1168    1416    1168 
   Size:        248    3489
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3737
 
NgoMIV G'CCGG_C

Cuts at:   1916    1916 
   Size:       3737
 
NspI r_CATG'y

Cuts at:    354     790    1290    2197     354 
   Size:        436     500     907    1894

  Fragments arranged by size: 

                1894     907     500     436
 
PciI A'CATG_T

Cuts at:   2193    2193 
   Size:       3737
 
PmeI GTTT'AAAC

Cuts at:    736     736 
   Size:       3737
 
PmlI CAC'GTG

Cuts at:    414     414 
   Size:       3737
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2876    2908    2876 
   Size:         32    3705
 
PpuMI rG'GwC_Cy

Cuts at:    582     582 
   Size:       3737
 
PstI C_TGCA'G

Cuts at:    784    1469     784 
   Size:        685    3052
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3737
 
PvuII CAG'CTG

Cuts at:    313    1162    1522     313 
   Size:        849     360    2528

  Fragments arranged by size: 

                2528     849     360
 
RsrII CG'GwC_CG

Cuts at:   1932    1932 
   Size:       3737
 
SacI G_AGCT'C

Cuts at:    751     751 
   Size:       3737
 
SalI G'TCGA_C

Cuts at:    598     774     598 
   Size:        176    3561
 
SapI GCTCTTCn'nnn_

Cuts at:   1760    1970    1760 
   Size:        210    3527
 
SbfI CC_TGCA'GG

Cuts at:    784     784 
   Size:       3737
 
ScaI AGT'ACT

Cuts at:   3254    3254 
   Size:       3737
 
SfcI C'TryA_G

Cuts at:     33     780    1465    2458    2649      33 
   Size:        747     685     993     191    1121

  Fragments arranged by size: 

                1121     993     747     685     191
 
SfoI GGC'GCC

Cuts at:   1169    1417    1169 
   Size:        248    3489
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3737
 
SmaI CCC'GGG

Cuts at:    759     759 
   Size:       3737
 
SmlI C'TyrA_G

Cuts at:    504     892    1807    2299    2561     504 
   Size:        388     915     492     262    1680

  Fragments arranged by size: 

                1680     915     492     388     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3737
 
SphI G_CATG'C

Cuts at:    790    1290     790 
   Size:        500    3237
 
SspI AAT'ATT

Cuts at:    555     555 
   Size:       3737
 
StyI C'CwwG_G

Cuts at:    107     578     871    1225     107 
   Size:        471     293     354    2619

  Fragments arranged by size: 

                2619     471     354     293
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    721    1320    1453    1912    2121     721 
   Size:        599     133     459     209    2337

  Fragments arranged by size: 

                2337     599     459     209     133
 
TatI w'GTAC_w

Cuts at:   3252    3252 
   Size:       3737
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3254    3286    3254 
   Size:         32    3705
 
Tth111I GACn'n_nGTC

Cuts at:   1534    1534 
   Size:       3737
 
XbaI T'CTAG_A

Cuts at:    768     768 
   Size:       3737
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    150    1232     150 
   Size:       1082    2655
 
XmaI C'CCGG_G

Cuts at:    757     757 
   Size:       3737
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI     FspI    FspAI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PciI     PmeI     PmlI     PpiI
    PpuMI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     SspI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI    BmgBI
     BmtI     BpmI   Bpu10I    BsaXI    BseRI     BsgI    BsiWI     BsmI
    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI
    CspCI   DraIII    EcoNI    EcoRV     FseI  HindIII     HpaI     MfeI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI
    SexAI     SfiI    SgrAI    SnaBI     SrfI     StuI     SwaI     XhoI
     XmnI     ZraI
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