################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I CelII ClaI CpoI DraI DrdI Eam1105I EarI Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HinfI HpaI KpnI Ksp632I MaeI MamI MfeI MflI MluI MroI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SalI SapI SbfI ScaI SfaNI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 435 GACGTC GACGT^C AocI 271 CCTGAGG CC^T[ACGT]AGG ApaI 29 GGGCCC GGGCC^C AvaI 280 CCCGAG C^[CT]CG[GA]G BbsI 241 GTCTTC GAAGAC(2/6) BglI 652 GCCGCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 241 GTCTTC GAAGAC(2/6) BsaAI 424 TACGTG [CT]AC^GT[GA] BsmAI 775 GAGAC GTCTC(1/5) BsoBI 280 CCCGAG C^[CT]CG[GA]G BsrDI 730 GCAATG GCAATG(2/0) BsrFI 361 GCCGGC [GA]^CCGG[CT] BssSI 331 CTCGTG CACGAG(-5/-1) BstF5I 54 GGATG GGATG(2/0) Bsu36I 271 CCTGAGG CC^T[ACGT]AGG Cfr10I 361 GCCGGC [GA]^CCGG[CT] DpnII 640 GATC ^GATC DsaI 685 CCGCGG C^C[GA][CT]GG Eco81I 271 CCTGAGG CC^T[ACGT]AGG EcoNI 195 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 236 CCAAGG C^C[AT][AT]GG FokI 54 GGATG GGATG(9/13) KspI 685 CCGCGG CCGC^GG MboI 640 GATC ^GATC MslI 612 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 685 CCGCGG C[AC]G^C[GT]G NaeI 361 GCCGGC GCC^GGC NdeII 640 GATC ^GATC NgoMIV 361 GCCGGC G^CCGGC PstI 196 CTGCAG CTGCA^G SacI 669 GAGCTC GAGCT^C SacII 685 CCGCGG CCGC^GG Sau3AI 640 GATC ^GATC SexAI 164 ACCTGGT A^CC[AT]GGT SfcI 196 CTGCAG C^T[GA][CT]AG StuI 922 AGGCCT AGG^CCT StyI 236 CCAAGG C^C[AT][AT]GG TaqI 65 TCGA T^CGA TthHB8I 65 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BcnI 357 CCCGG CC^[GC]GG 702 CCGGG BseRI 13 CTCCTC GAGGAG(10/8) 127 GAGGAG BspMI 405 GCAGGT ACCTGC(4/8) 882 GCAGGT BsrI 717 CCAGT ACTGG(1/-1) 862 CCAGT DraII 28 GGGGCCC [GA]G^G[ACGT]CC[CT] 29 GGGCCCT DraIII 163 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG 615 CACCATGTG EcoO109I 28 GGGGCCC [GA]G^G[ACGT]CC[CT] 29 GGGCCCT HgaI 150 GCGTC GACGC(5/10) 623 GCGTC MaeIII 678 GTCAC ^GT[ACGT]AC 865 GTCAC NciI 357 CCCGG CC^[GC]GG 702 CCGGG Tsp45I 678 GTCAC ^GT[GC]AC 865 GTCAC TspRI 174 CACTG CA[GC]TG[ACGT][ACGT]^ 183 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AccII AciI AcyI AluI AspHI BalI BanI BanII BbeI BbvI BmyI BpmI BsaHI BsaJI BsgI BsiEI BsiHKAI BsiYI BslI BsmFI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI EaeI EagI EclXI Eco52I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI KasI MaeII MboII MluNI MnlI MscI MspI MvaI MvnI MwoI NarI NlaIII NlaIV Sau96I ScrFI SfoI TseI XcmI