################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## Acc65I AccI AclI AcsI AflII AgeI AhdI Alw44I AlwI AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BamHI BbrPI BclI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaBI BseRI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspLU11I BsrGI BssHII Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I KspI MamI MfeI MflI MluI MseI MunI NciI NdeI NheI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AatII 673 GACGTC GACGT^C AccIII 8 TCCGGA T^CCGGA AflIII 716 ACGTGT A^C[GA][CT]GT Aor51HI 1238 AGCGCT AGC^GCT AspI 183 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 418 GGCGCC GGCGC^C BbsI 838 GTCTTC GAAGAC(2/6) BcgI 1159 CGATGTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 242 GGATAC GTATCC(6/5) BpuAI 838 GTCTTC GAAGAC(2/6) BsaI 135 GAGACC GGTCTC(1/5) BsaWI 8 TCCGGA [AT]^CCGG[AT] BseAI 8 TCCGGA T^CCGGA BsmAI 135 GAGAC GTCTC(1/5) BspEI 8 TCCGGA T^CCGGA BsrDI 1203 CATTGC GCAATG(2/0) BstAPI 452 GCAGCTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1307 CCATGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DpnII 195 GATC ^GATC Eco47III 1238 AGCGCT AGC^GCT KasI 418 GGCGCC G^GCGCC MboI 195 GATC ^GATC MroI 8 TCCGGA T^CCGGA MspA1I 1136 CAGCGG C[AC]G^C[GT]G NarI 418 GGCGCC GG^CGCC NdeII 195 GATC ^GATC NotI 552 GCGGCCGC GC^GGCCGC PflFI 183 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 278 GGGACCC [GA]G^G[AT]CC[CT] PshAI 918 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Sau3AI 195 GATC ^GATC ScaI 899 AGTACT AGT^ACT SexAI 218 ACCAGGT A^CC[AT]GGT SfoI 418 GGCGCC GGC^GCC SmlI 91 CTCAAG C^T[CT][GA]AG Tth111I 183 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AfaI 349 GTAC GT^AC 900 GTAC ApaI 1010 GGGCCC GGGCC^C 1140 GGGCCC AspHI 637 GTGCTC G[AT]GC[AT]^C 742 GTGCTC BalI 908 TGGCCA TGG^CCA 1292 TGGCCA BanI 418 GGCGCC G^G[CT][GA]CC 536 GGCACC BbvCI 21 CCTCAGC CCTCAGC(-5/-2) 73 GCTGAGG BfaI 1007 CTAG C^TAG 1100 CTAG BpmI 377 CTCCAG CTGGAG(16/14) 175 CTGGAG BsaAI 715 TACGTG [CT]AC^GT[GA] 1192 CACGTA BsgI 1186 CTGCAC GTGCAG(16/14) 1339 GTGCAG BsiEI 553 CGGCCG CG[GA][CT]^CG 872 CGGCCG BsiHKAI 637 GTGCTC G[AT]GC[AT]^C 742 GTGCTC BspHI 956 TCATGA T^CATGA 1319 TCATGA BsrBI 763 CCGCTC CCGCTC(-3/-3) 886 CCGCTC BssSI 1150 CACGAG CACGAG(-5/-1) 456 CTCGTG DsaI 920 CCATGG C^C[GA][CT]GG 1307 CCATGG EagI 553 CGGCCG C^GGCCG 872 CGGCCG EclXI 553 CGGCCG C^GGCCG 872 CGGCCG Eco52I 553 CGGCCG C^GGCCG 872 CGGCCG EcoNI 1001 CCTGGGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1114 CCTGAAGGAGG EcoT14I 920 CCATGG C^C[AT][AT]GG 1307 CCATGG MaeI 1007 CTAG C^TAG 1100 CTAG MluNI 908 TGGCCA TGG^CCA 1292 TGGCCA MscI 908 TGGCCA TGG^CCA 1292 TGGCCA NcoI 920 CCATGG C^CATGG 1307 CCATGG NspI 617 GCATGC [GA]CATG^[CT] 863 GCATGC PstI 56 CTGCAG CTGCA^G 566 CTGCAG RcaI 956 TCATGA T^CATGA 1319 TCATGA RsaI 349 GTAC GT^AC 900 GTAC SfcI 56 CTGCAG C^T[GA][CT]AG 566 CTGCAG SphI 617 GCATGC GCATG^C 863 GCATGC StyI 920 CCATGG C^C[AT][AT]GG 1307 CCATGG TaqI 200 TCGA T^CGA 488 TCGA TthHB8I 200 TCGA T^CGA 488 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AccII AciI AcyI AluI AlwNI AvaII BanII BbvI BglI BmyI BsaHI BsaJI BsiYI BslI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboII MnlI MslI MspI MvaI MvnI MwoI NaeI NgoMIV NlaIII NlaIV PleI Sau96I ScrFI SfaNI TseI Tsp45I TspRI XcmI