(Circular) (Six-base) MAPSORT of: pF1KE0266.seq Check: 7433 from: 1 to: 4463 pF1KE0266 4463 bp With 182 enzymes: SgfI * April 24, 2009 13:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 893 893 Size: 4463 AatII G_ACGT'C Cuts at: 785 785 Size: 4463 AccI GT'mk_AC Cuts at: 1501 1501 Size: 4463 Acc65I G'GTAC_C Cuts at: 1479 1479 Size: 4463 AclI AA'CG_TT Cuts at: 4303 4303 Size: 4463 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1244 2288 2720 3467 3735 1244 Size: 1044 432 747 268 1972 Fragments arranged by size: 1972 1044 747 432 268 AfeI AGC'GCT Cuts at: 1348 1348 Size: 4463 AflIII A'CryG_T Cuts at: 824 1815 2919 3896 824 Size: 991 1104 977 1391 Fragments arranged by size: 1391 1104 991 977 AgeI A'CCGG_T Cuts at: 2851 2851 Size: 4463 AleI CACnn'nnGTG Cuts at: 505 505 Size: 4463 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 853 853 Size: 4463 AlwNI CAG_nnn'CTG Cuts at: 270 333 957 1455 1768 3335 270 Size: 63 624 498 313 1567 1398 Fragments arranged by size: 1567 1398 624 498 313 63 ApaI G_GGCC'C Cuts at: 1122 1252 1122 Size: 130 4333 ApaLI G'TGCA_C Cuts at: 3233 3233 Size: 4463 ApoI r'AATT_y Cuts at: 1467 3816 4072 1467 Size: 2349 256 1858 Fragments arranged by size: 2349 1858 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4463 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4463 AvaI C'yCGr_G Cuts at: 1483 1483 Size: 4463 BamHI G'GATC_C Cuts at: 1488 1488 Size: 4463 BanI G'GyrC_C Cuts at: 526 644 1479 1893 2141 2176 526 Size: 118 835 414 248 35 2813 Fragments arranged by size: 2813 835 414 248 118 35 BanII G_rGCy'C Cuts at: 116 773 1122 1175 1252 1477 2507 116 Size: 657 349 53 77 225 1030 2072 Fragments arranged by size: 2072 1030 657 349 225 77 53 BbeI G_GCGC'C Cuts at: 530 1897 2145 530 Size: 1367 248 2848 Fragments arranged by size: 2848 1367 248 BbsI GAAGACnn'nnnn_ Cuts at: 939 939 Size: 4463 BbvCI CC'TCA_GC Cuts at: 130 182 130 Size: 52 4411 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1256 1290 1256 Size: 34 4429 BciVI GTATCCnnnnn_n' Cuts at: 344 2361 3122 4463 344 Size: 2017 761 1341 344 Fragments arranged by size: 2017 1341 761 344 BclI T'GATC_A Cuts at: 1983 1983 Size: 4463 BglI GCCn_nnn'nGGC Cuts at: 524 685 735 1179 524 Size: 161 50 444 3808 Fragments arranged by size: 3808 444 161 50 BglII A'GATC_T Cuts at: 1978 4015 1978 Size: 2037 2426 BlpI GC'TnA_GC Cuts at: 1575 1575 Size: 4463 Bme1580I G_kGCm'C Cuts at: 240 647 1122 1252 2088 2181 3237 240 Size: 407 475 130 836 93 1056 1466 Fragments arranged by size: 1466 1056 836 475 407 130 93 BmrI ACTGGGnnnn_n' Cuts at: 1082 1093 1209 1675 1756 2091 4235 1082 Size: 11 116 466 81 335 2144 1310 Fragments arranged by size: 2144 1310 466 335 116 81 11 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2830 2830 Size: 4463 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 304 470 304 Size: 166 4297 Bpu10I CC'TnA_GC Cuts at: 130 182 130 Size: 52 4411 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 184 1639 2518 3010 3308 184 Size: 1455 879 492 298 1339 Fragments arranged by size: 1455 1339 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 237 4167 237 Size: 3930 533 BsaAI yAC'GTr Cuts at: 825 1302 1816 825 Size: 477 514 3472 Fragments arranged by size: 3472 514 477 BsaBI GATnn'nnATC Cuts at: 6 1977 4014 6 Size: 1971 2037 455 Fragments arranged by size: 2037 1971 455 BsaHI Gr'CG_yC Cuts at: 527 599 782 920 1894 2142 2844 4340 527 Size: 72 183 138 974 248 702 1496 650 Fragments arranged by size: 1496 974 702 650 248 183 138 72 BsaWI w'CCGG_w Cuts at: 116 1652 2173 2851 3125 3272 3683 116 Size: 1536 521 678 274 147 411 896 Fragments arranged by size: 1536 896 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 427 536 602 720 915 1128 1242 1343 1888 Size: 109 66 118 195 213 114 101 545 Cuts at: 1888 2646 3223 3719 427 Size: 758 577 496 1171 Fragments arranged by size: 1171 758 577 545 496 213 195 118 114 109 101 66 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1279 1468 1279 Size: 189 4274 BsiEI CG_ry'CG Cuts at: 104 664 983 2005 2051 3259 104 Size: 560 319 1022 46 1208 1308 Fragments arranged by size: 1308 1208 1022 560 319 46 BsiHKAI G_wGCw'C Cuts at: 749 854 1477 2255 2445 3237 749 Size: 105 623 778 190 792 1975 Fragments arranged by size: 1975 792 778 623 190 105 Bsp1286I G_dGCh'C Cuts at: 116 240 647 749 773 854 1122 1175 1252 Size: 124 407 102 24 81 268 53 77 Cuts at: 1252 1477 2088 2181 2255 2445 2507 3237 116 Size: 225 611 93 74 190 62 730 1342 Fragments arranged by size: 1342 730 611 407 268 225 190 124 102 93 81 77 74 62 53 24 BspEI T'CCGG_A Cuts at: 116 3683 116 Size: 3567 896 BspHI T'CATG_A Cuts at: 1064 1427 3639 1064 Size: 363 2212 1888 Fragments arranged by size: 2212 1888 363 BspMI ACCTGCnnnn'nnnn_ Cuts at: 842 893 1079 1513 1727 2029 2410 842 Size: 51 186 434 214 302 381 2895 Fragments arranged by size: 2895 434 381 302 214 186 51 BsrBI CCG'CTC Cuts at: 50 873 996 2755 2809 4293 4458 50 Size: 823 123 1759 54 1484 165 55 Fragments arranged by size: 1759 1484 823 165 123 55 54 BsrDI GCAATG_nn' Cuts at: 1310 2375 1310 Size: 1065 3398 BsrFI r'CCGG_y Cuts at: 188 546 729 978 2461 2642 2851 188 Size: 358 183 249 1483 181 209 1800 Fragments arranged by size: 1800 1483 358 249 209 183 181 BssSI C'ACGA_G Cuts at: 564 1258 2734 3092 564 Size: 694 1476 358 1935 Fragments arranged by size: 1935 1476 694 358 BstAPI GCAn_nnn'nTGC Cuts at: 566 1840 566 Size: 1274 3189 BstBI TT'CG_AA Cuts at: 2824 2824 Size: 4463 BstXI CCAn_nnnn'nTGG Cuts at: 1422 1422 Size: 4463 BstYI r'GATC_y Cuts at: 1 1488 1955 1978 2313 2559 3571 3657 3669 Size: 1487 467 23 335 246 1012 86 12 Cuts at: 3669 4015 1 Size: 346 449 Fragments arranged by size: 1487 1012 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 1028 1415 107 Size: 921 387 3155 Fragments arranged by size: 3155 921 387 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2413 2594 2757 2413 Size: 181 163 4119 Fragments arranged by size: 4119 181 163 BtsI GCAGTG_nn' Cuts at: 606 1706 606 Size: 1100 3363 DraI TTT'AAA Cuts at: 1462 1462 Size: 4463 DrdI GACnn_nn'nnGTC Cuts at: 2169 3027 2169 Size: 858 3605 EaeI y'GGCC_r Cuts at: 445 548 661 962 980 1016 1400 2048 2222 Size: 103 113 301 18 36 384 648 174 Cuts at: 2222 2613 2640 3985 445 Size: 391 27 1345 923 Fragments arranged by size: 1345 923 648 391 384 301 174 113 103 36 27 18 EagI C'GGCC_G Cuts at: 661 980 2048 661 Size: 319 1068 3076 Fragments arranged by size: 3076 1068 319 EarI CTCTTCn'nnn_ Cuts at: 2486 2696 2486 Size: 210 4253 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 483 703 1084 2671 2981 3127 4273 483 Size: 220 381 1587 310 146 1146 673 Fragments arranged by size: 1587 1146 673 381 310 220 146 EcoICRI GAG'CTC Cuts at: 1475 1475 Size: 4463 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 304 470 1244 2288 2720 3467 3735 304 Size: 166 774 1044 432 747 268 1032 Fragments arranged by size: 1044 1032 774 747 432 268 166 EcoNI CCTnn'n_nnAGG Cuts at: 1113 1226 1113 Size: 113 4350 EcoO109I rG'GnC_Cy Cuts at: 387 1118 1119 1146 1249 1452 1602 387 Size: 731 1 27 103 203 150 3248 Fragments arranged by size: 3248 731 203 150 103 27 1 EcoRI G'AATT_C Cuts at: 1467 1467 Size: 4463 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2482 2482 Size: 4463 FspI TGC'GCA Cuts at: 2244 2244 Size: 4463 HaeII r_GCGC'y Cuts at: 530 674 940 1350 1371 1897 2145 3167 4146 Size: 144 266 410 21 526 248 1022 979 Cuts at: 4146 530 Size: 847 Fragments arranged by size: 1022 979 847 526 410 266 248 144 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 327 359 895 927 2305 2335 2337 2367 327 Size: 32 536 32 1378 30 2 30 2423 Fragments arranged by size: 2423 1378 536 32 32 30 30 2 HincII GTy'rAC Cuts at: 1502 1502 Size: 4463 KasI G'GCGC_C Cuts at: 526 1893 2141 526 Size: 1367 248 2848 Fragments arranged by size: 2848 1367 248 KpnI G_GTAC'C Cuts at: 1483 1483 Size: 4463 MluI A'CGCG_T Cuts at: 3896 3896 Size: 4463 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3134 3318 3134 Size: 184 4279 MscI TGG'CCA Cuts at: 1018 1402 2224 1018 Size: 384 822 3257 Fragments arranged by size: 3257 822 384 MslI CAynn'nnrTG Cuts at: 310 505 1079 2452 2579 310 Size: 195 574 1373 127 2194 Fragments arranged by size: 2194 1373 574 195 127 MspA1I CmG'CkG Cuts at: 1246 1574 1888 2248 3261 3506 1246 Size: 328 314 360 1013 245 2203 Fragments arranged by size: 2203 1013 360 328 314 245 NaeI GCC'GGC Cuts at: 190 548 731 2644 190 Size: 358 183 1913 2009 Fragments arranged by size: 2009 1913 358 183 NarI GG'CG_CC Cuts at: 527 1894 2142 527 Size: 1367 248 2848 Fragments arranged by size: 2848 1367 248 NcoI C'CATG_G Cuts at: 107 1028 1415 107 Size: 921 387 3155 Fragments arranged by size: 3155 921 387 NgoMIV G'CCGG_C Cuts at: 188 546 729 2642 188 Size: 358 183 1913 2009 Fragments arranged by size: 2009 1913 358 183 NotI GC'GGCC_GC Cuts at: 661 661 Size: 4463 NspI r_CATG'y Cuts at: 729 975 1516 2016 2923 729 Size: 246 541 500 907 2269 Fragments arranged by size: 2269 907 541 500 246 PciI A'CATG_T Cuts at: 2919 2919 Size: 4463 PfoI T'CCnGG_A Cuts at: 628 628 Size: 4463 PmeI GTTT'AAAC Cuts at: 1462 1462 Size: 4463 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3602 3634 3602 Size: 32 4431 PpuMI rG'GwC_Cy Cuts at: 387 387 Size: 4463 PshAI GACnn'nnGTC Cuts at: 1030 1030 Size: 4463 PspOMI G'GGCC_C Cuts at: 1118 1248 1118 Size: 130 4333 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 1060 1092 1060 Size: 32 4431 PstI C_TGCA'G Cuts at: 168 678 1510 2195 168 Size: 510 832 685 2436 Fragments arranged by size: 2436 832 685 510 PvuI CG_AT'CG Cuts at: 104 104 Size: 4463 PvuII CAG'CTG Cuts at: 1888 2248 1888 Size: 360 4103 RsrII CG'GwC_CG Cuts at: 2658 2658 Size: 4463 SacI G_AGCT'C Cuts at: 1477 1477 Size: 4463 SalI G'TCGA_C Cuts at: 1500 1500 Size: 4463 SanDI GG'GwC_CC Cuts at: 387 387 Size: 4463 SapI GCTCTTCn'nnn_ Cuts at: 2486 2696 2486 Size: 210 4253 SbfI CC_TGCA'GG Cuts at: 1510 1510 Size: 4463 ScaI AGT'ACT Cuts at: 1009 3980 1009 Size: 2971 1492 SexAI A'CCwGG_T Cuts at: 326 326 Size: 4463 SfcI C'TryA_G Cuts at: 33 164 674 1506 2191 3184 3375 33 Size: 131 510 832 685 993 191 1121 Fragments arranged by size: 1121 993 832 685 510 191 131 SfoI GGC'GCC Cuts at: 528 1895 2143 528 Size: 1367 248 2848 Fragments arranged by size: 2848 1367 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4463 SmaI CCC'GGG Cuts at: 1485 1485 Size: 4463 SmlI C'TyrA_G Cuts at: 199 1618 2533 3025 3287 199 Size: 1419 915 492 262 1375 Fragments arranged by size: 1419 1375 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4463 SphI G_CATG'C Cuts at: 729 975 1516 2016 729 Size: 246 541 500 3176 Fragments arranged by size: 3176 541 500 246 StyI C'CwwG_G Cuts at: 107 1028 1415 1597 1951 107 Size: 921 387 182 354 2619 Fragments arranged by size: 2619 921 387 354 182 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2046 2179 2638 2847 2046 Size: 133 459 209 3662 Fragments arranged by size: 3662 459 209 133 TatI w'GTAC_w Cuts at: 1007 3978 1007 Size: 2971 1492 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1040 1072 3980 4012 1040 Size: 32 2908 32 1491 Fragments arranged by size: 2908 1491 32 32 Tth111I GACn'n_nGTC Cuts at: 294 2260 294 Size: 1966 2497 XbaI T'CTAG_A Cuts at: 1494 1494 Size: 4463 XcmI CCAnnnn_n'nnnnTGG Cuts at: 504 1026 1106 1958 504 Size: 522 80 852 3009 Fragments arranged by size: 3009 852 522 80 XmaI C'CCGG_G Cuts at: 1483 1483 Size: 4463 ZraI GAC'GTC Cuts at: 783 783 Size: 4463 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PciI PfoI PmeI PpiI PpuMI PshAI PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI Enzymes that do not cut: AflII AhdI AloI AscI AvrII BaeI BfrBI BmgBI BmtI BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NruI NsiI PacI PasI PflMI PmlI PsiI PspXI SacII SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI