Restriction map for pF1KE0260
 (Linear) (Six-base) MAPSORT of: KIEE0260.seq  Check: 1943  from: 1  to: 294



KIEE0260.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     201     294 
   Size:        201      93
 
BglII A'GATC_T

Cuts at:      0     263     294 
   Size:        263      31
 
BsaWI w'CCGG_w

Cuts at:      0     123     294 
   Size:        123     171
 
BspEI T'CCGG_A

Cuts at:      0     123     294 
   Size:        123     171
 
BstYI r'GATC_y

Cuts at:      0     263     294 
   Size:        263      31
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     201     294 
   Size:        201      93
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     248     280     294 
   Size:        248      32      14

  Fragments arranged by size: 

                 248      32      14
 
PfoI T'CCnGG_A

Cuts at:      0     164     294 
   Size:        164     130
 
SfcI C'TryA_G

Cuts at:      0      84     294 
   Size:         84     210
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     177     209     294 
   Size:        177      32      85

  Fragments arranged by size: 

                 177      85      32
 

 Enzymes that do cut:

     AcuI    BglII    BsaWI    BspEI    BstYI  Eco57MI    Hin4I     PfoI
     SfcI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI
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