Restriction map for
pF1KE0246
(Linear) (Six-base) MAPSORT of: KIEE0246.seq Check: 5223 from: 1 to: 1254
KIEE0246.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AfeI AGC'GCT
Cuts at: 0 496 1254
Size: 496 758
AflIII A'CryG_T
Cuts at: 0 122 1254
Size: 122 1132
AhdI GACnn_n'nnGTC
Cuts at: 0 1203 1254
Size: 1203 51
AlwNI CAG_nnn'CTG
Cuts at: 0 288 891 1254
Size: 288 603 363
Fragments arranged by size:
603 363 288
ApaLI G'TGCA_C
Cuts at: 0 388 669 828 1254
Size: 388 281 159 426
Fragments arranged by size:
426 388 281 159
AvaI C'yCGr_G
Cuts at: 0 30 96 1185 1254
Size: 30 66 1089 69
Fragments arranged by size:
1089 69 66 30
BanI G'GyrC_C
Cuts at: 0 144 600 965 1254
Size: 144 456 365 289
Fragments arranged by size:
456 365 289 144
BbeI G_GCGC'C
Cuts at: 0 969 1254
Size: 969 285
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 849 883 1254
Size: 849 34 371
Fragments arranged by size:
849 371 34
BglI GCCn_nnn'nGGC
Cuts at: 0 595 1135 1254
Size: 595 540 119
Fragments arranged by size:
595 540 119
Bme1580I G_kGCm'C
Cuts at: 0 37 232 392 673 832 1254
Size: 37 195 160 281 159 422
Fragments arranged by size:
422 281 195 160 159 37
BmgBI CAC'GTC
Cuts at: 0 165 1254
Size: 165 1089
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 1209 1254
Size: 97 1112 45
Fragments arranged by size:
1112 97 45
BsaAI yAC'GTr
Cuts at: 0 916 960 1254
Size: 916 44 294
Fragments arranged by size:
916 294 44
BsaHI Gr'CG_yC
Cuts at: 0 413 446 966 1254
Size: 413 33 520 288
Fragments arranged by size:
520 413 288 33
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 166 196 1051 1081 1207 1237 1254
Size: 166 30 855 30 126 30 17
Fragments arranged by size:
855 166 126 30 30 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 1254
Size: 94 1160
BseYI C'CCAG_C
Cuts at: 0 148 1254
Size: 148 1106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 435 938 1254
Size: 435 503 316
Fragments arranged by size:
503 435 316
BsiEI CG_ry'CG
Cuts at: 0 44 812 836 1254
Size: 44 768 24 418
Fragments arranged by size:
768 418 44 24
BsiHKAI G_wGCw'C
Cuts at: 0 298 392 673 832 848 1254
Size: 298 94 281 159 16 406
Fragments arranged by size:
406 298 281 159 94 16
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1104 1237 1254
Size: 1104 133 17
Fragments arranged by size:
1104 133 17
Bsp1286I G_dGCh'C
Cuts at: 0 37 232 298 392 673 832 848 1254
Size: 37 195 66 94 281 159 16 406
Fragments arranged by size:
406 281 195 159 94 66 37 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 344 1254
Size: 344 910
BsrDI GCAATG_nn'
Cuts at: 0 1236 1254
Size: 1236 18
BsrFI r'CCGG_y
Cuts at: 0 39 415 655 1254
Size: 39 376 240 599
Fragments arranged by size:
599 376 240 39
BsrGI T'GTAC_A
Cuts at: 0 371 1088 1254
Size: 371 717 166
Fragments arranged by size:
717 371 166
BstAPI GCAn_nnn'nTGC
Cuts at: 0 288 903 1254
Size: 288 615 351
Fragments arranged by size:
615 351 288
BstEII G'GTnAC_C
Cuts at: 0 195 1041 1254
Size: 195 846 213
Fragments arranged by size:
846 213 195
BstXI CCAn_nnnn'nTGG
Cuts at: 0 192 363 1254
Size: 192 171 891
Fragments arranged by size:
891 192 171
BtgI C'CryG_G
Cuts at: 0 362 423 461 617 743 1254
Size: 362 61 38 156 126 511
Fragments arranged by size:
511 362 156 126 61 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 759 912 1254
Size: 759 153 342
Fragments arranged by size:
759 342 153
BtsI GCAGTG_nn'
Cuts at: 0 686 926 1003 1254
Size: 686 240 77 251
Fragments arranged by size:
686 251 240 77
DraIII CAC_nnn'GTG
Cuts at: 0 903 1254
Size: 903 351
DrdI GACnn_nn'nnGTC
Cuts at: 0 537 1254
Size: 537 717
EaeI y'GGCC_r
Cuts at: 0 41 353 476 503 645 809 863 1127
Size: 41 312 123 27 142 164 54 264
Cuts at: 1127 1254
Size: 127
Fragments arranged by size:
312 264 164 142 127 123 54 41
27
EagI C'GGCC_G
Cuts at: 0 41 809 1254
Size: 41 768 445
Fragments arranged by size:
768 445 41
EarI CTCTTCn'nnn_
Cuts at: 0 223 1060 1165 1254
Size: 223 837 105 89
Fragments arranged by size:
837 223 105 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1102 1254
Size: 1102 152
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 1209 1254
Size: 97 1112 45
Fragments arranged by size:
1112 97 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1185 1254
Size: 1185 69
EcoO109I rG'GnC_Cy
Cuts at: 0 888 1254
Size: 888 366
HaeII r_GCGC'y
Cuts at: 0 88 217 328 498 572 641 969 1254
Size: 88 129 111 170 74 69 328 285
Fragments arranged by size:
328 285 170 129 111 88 74 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 1189 1221 1254
Size: 166 32 991 32 33
Fragments arranged by size:
991 166 33 32 32
HincII GTy'rAC
Cuts at: 0 717 1254
Size: 717 537
KasI G'GCGC_C
Cuts at: 0 965 1254
Size: 965 289
MluI A'CGCG_T
Cuts at: 0 122 1254
Size: 122 1132
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 356 1254
Size: 107 249 898
Fragments arranged by size:
898 249 107
MscI TGG'CCA
Cuts at: 0 355 478 505 865 1129 1254
Size: 355 123 27 360 264 125
Fragments arranged by size:
360 355 264 125 123 27
MslI CAynn'nnrTG
Cuts at: 0 361 385 724 1254
Size: 361 24 339 530
Fragments arranged by size:
530 361 339 24
MspA1I CmG'CkG
Cuts at: 0 60 425 1254
Size: 60 365 829
Fragments arranged by size:
829 365 60
NaeI GCC'GGC
Cuts at: 0 41 417 657 1254
Size: 41 376 240 597
Fragments arranged by size:
597 376 240 41
NarI GG'CG_CC
Cuts at: 0 966 1254
Size: 966 288
NcoI C'CATG_G
Cuts at: 0 617 743 1254
Size: 617 126 511
Fragments arranged by size:
617 511 126
NgoMIV G'CCGG_C
Cuts at: 0 39 415 655 1254
Size: 39 376 240 599
Fragments arranged by size:
599 376 240 39
PasI CC'CwG_GG
Cuts at: 0 398 1254
Size: 398 856
PmlI CAC'GTG
Cuts at: 0 960 1254
Size: 960 294
PstI C_TGCA'G
Cuts at: 0 284 293 1254
Size: 284 9 961
Fragments arranged by size:
961 284 9
SacII CC_GC'GG
Cuts at: 0 426 1254
Size: 426 828
SapI GCTCTTCn'nnn_
Cuts at: 0 223 1254
Size: 223 1031
SfcI C'TryA_G
Cuts at: 0 280 289 1254
Size: 280 9 965
Fragments arranged by size:
965 280 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1135 1254
Size: 1135 119
SfoI GGC'GCC
Cuts at: 0 967 1254
Size: 967 287
SgrAI Cr'CCGG_yG
Cuts at: 0 655 1254
Size: 655 599
SmaI CCC'GGG
Cuts at: 0 32 98 1254
Size: 32 66 1156
Fragments arranged by size:
1156 66 32
SmlI C'TyrA_G
Cuts at: 0 1185 1254
Size: 1185 69
StyI C'CwwG_G
Cuts at: 0 185 617 743 1254
Size: 185 432 126 511
Fragments arranged by size:
511 432 185 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 162 375 747 1254
Size: 162 213 372 507
Fragments arranged by size:
507 372 213 162
TatI w'GTAC_w
Cuts at: 0 371 1088 1254
Size: 371 717 166
Fragments arranged by size:
717 371 166
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 982 1014 1207 1239 1254
Size: 982 32 193 32 15
Fragments arranged by size:
982 193 32 32 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 156 486 1254
Size: 156 330 768
Fragments arranged by size:
768 330 156
XhoI C'TCGA_G
Cuts at: 0 1185 1254
Size: 1185 69
XmaI C'CCGG_G
Cuts at: 0 30 96 1254
Size: 30 66 1158
Fragments arranged by size:
1158 66 30
Enzymes that do cut:
AfeI AflIII AhdI AlwNI ApaLI AvaI BanI BbeI
BcgI BglI Bme1580I BmgBI BpmI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrDI BsrFI BsrGI BstAPI BstEII BstXI BtgI BtgZI
BtsI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII KasI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV PasI
PmlI PstI SacII SapI SfcI SfiI SfoI SgrAI
SmaI SmlI StyI TaqII TatI TstI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AleI AlfI AloI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanII BbsI BbvCI BciVI BclI
BfrBI BglII BlpI BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrBI
BssHII BssSI BstBI BstYI BstZ17I Bsu36I ClaI CspCI
DraI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI NdeI NheI NotI NruI
NsiI NspI PacI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SalI SanDI SbfI ScaI SexAI SgfI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XmnI ZraI