Restriction map for pF1KE0245
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
AspEI       AspHI       AspI        AviII       AvrII       
BamHI       BbeI        BbrPI       BbsI        BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BseAI       BsiEI       BsiHKAI     BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstUI       BstXI       BstYI       
BstZ17I     CelII       ClaI        CpoI        DpnII       
DraI        DraIII      DrdI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       HindIII     HpaI        KasI        Ksp632I     
KspI        MaeII       MamI        MboI        MboII       
MfeI        MflI        MluI        MroI        MseI        
MslI        MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
ScaI        SexAI       SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        SspBI       
SspI        StuI        SwaI        TfiI        Tru9I       
Tsp509I     Tth111I     Van91I      XbaI        XhoI        
XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

Acc65I        688  GGTACC               G^GTACC
AcyI          188  GGCGTC               G[GA]^CG[CT]C
AocI          366  CCTGAGG              CC^T[ACGT]AGG
ApaI          392  GGGCCC               GGGCC^C
Asp718I       688  GGTACC               G^GTACC
AvaII         223  GGACC                G^G[AT]CC
BalI          473  TGGCCA               TGG^CCA
BanI          688  GGTACC               G^G[CT][GA]CC
BanII         392  GGGCCC               G[GA]GC[CT]^C
BbvCI         375  CCTCAGC              CCTCAGC(-5/-2)
BfaI          193  CTAG                 C^TAG
BmyI          392  GGGCCC               G[AGT]GC[ACT]^C
BsaHI         188  GGCGTC               G[GA]^CG[CT]C
BsaWI          83  ACCGGA               [AT]^CCGG[AT]
BsiWI          15  CGTACG               C^GTACG
Bsp1286I      392  GGGCCC               G[AGT]GC[ACT]^C
BspMI         225  ACCTGC               ACCTGC(4/8)
BsrFI         316  ACCGGC               [GA]^CCGG[CT]
BsrI          185  ACTGG                ACTGG(1/-1)
Bsu36I        366  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        316  ACCGGC               [GA]^CCGG[CT]
DsaI          489  CCACGG               C^C[GA][CT]GG
Eco81I        366  CCTGAGG              CC^T[ACGT]AGG
EcoNI         362  CCTTCCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI          189  GCGTC                GACGC(5/10)
Hin1I         188  GGCGTC               G[GA]^CG[CT]C
HincII        274  GTCAAC               GT[CT]^[GA]AC
HindII        274  GTCAAC               GT[CT]^[GA]AC
HinfI         345  GACTC                G^A[ACGT]TC
KpnI          688  GGTACC               GGTAC^C
MaeI          193  CTAG                 C^TAG
MluNI         473  TGGCCA               TGG^CCA
MscI          473  TGGCCA               TGG^CCA
NlaIII        714  CATG                 CATG^
PleI          345  GACTC                GAGTC(4/5)
SbfI          226  CCTGCAGG             CCTGCA^GG
SfaNI         288  GCATC                GCATC(5/9)
SfiI          144  GGCCGGGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I      226  CCTGCAGG             CCTGCA^GG
TaqI          675  TCGA                 T^CGA
TthHB8I       675  TCGA                 T^CGA
XcmI          434  CCAAGGTGCAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

BglI          145  GCCGGGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              461  GCCCGCTGGGC         
BsaI          121  GGTCTC               GGTCTC(1/5)
              263  GGTCTC              
BseRI           4  GAGGAG               GAGGAG(10/8)
              523  GAGGAG              
BsmFI          72  GGGAC                GGGAC(10/14)
              593  GGGAC               
BssSI          63  CACGAG               CACGAG(-5/-1)
              268  CACGAG              
BstEII        204  GGTCACC              G^GT[ACGT]ACC
              402  GGTCACC             
BstPI         204  GGTCACC              G^GT[ACGT]ACC
              402  GGTCACC             
CfoI          286  GCGC                 GCG^C
              504  GCGC                
EaeI          152  TGGCCG               [CT]^GGCC[GA]
              473  TGGCCA              
EcoO65I       204  GGTCACC              G^GT[ACGT]ACC
              402  GGTCACC             
EcoT14I       159  CCAAGG               C^C[AT][AT]GG
              434  CCAAGG              
HhaI          286  GCGC                 GCG^C
              504  GCGC                
HinP1I        286  GCGC                 G^CGC
              504  GCGC                
PstI          175  CTGCAG               CTGCA^G
              227  CTGCAG              
SfcI          175  CTGCAG               C^T[GA][CT]AG
              227  CTGCAG              
SmaI          107  CCCGGG               CCC^GGG
              581  CCCGGG              
StyI          159  CCAAGG               C^C[AT][AT]GG
              434  CCAAGG              
XmaCI         107  CCCGGG               C^CCGGG
              581  CCCGGG              
XmaI          107  CCCGGG               C^CCGGG
              581  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AciI        AfaI        AluI        AvaI        BbvI        
BcnI        BpmI        BsaJI       BsgI        BsiYI       
BslI        BsmAI       BsoBI       BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DraII       
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HpaII       HphI        ItaI        
MaeIII      MnlI        MspA1I      MspI        MvaI        
MwoI        NciI        NlaIV       PvuII       RsaI        
Sau96I      ScrFI       TseI        Tsp45I      TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com