Restriction map for pF1KE0245
 (Linear) (Six-base) MAPSORT of: KIEE0245.seq  Check: 2862  from: 1  to: 720



KIEE0245.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

Acc65I G'GTAC_C

Cuts at:      0     688     720 
   Size:        688      32
 
ApaI G_GGCC'C

Cuts at:      0     396     720 
   Size:        396     324
 
AvaI C'yCGr_G

Cuts at:      0     107     520     581     720 
   Size:        107     413      61     139

  Fragments arranged by size: 

                 413     139     107      61
 
BanI G'GyrC_C

Cuts at:      0     688     720 
   Size:        688      32
 
BanII G_rGCy'C

Cuts at:      0     396     720 
   Size:        396     324
 
BbvCI CC'TCA_GC

Cuts at:      0     376     720 
   Size:        376     344
 
BglI GCCn_nnn'nGGC

Cuts at:      0     151     467     720 
   Size:        151     316     253

  Fragments arranged by size: 

                 316     253     151
 
Bme1580I G_kGCm'C

Cuts at:      0     396     720 
   Size:        396     324
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     291     550     646     720 
   Size:        291     259      96      74

  Fragments arranged by size: 

                 291     259      96      74
 
Bpu10I CC'TnA_GC

Cuts at:      0     376     720 
   Size:        376     344
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     127     269     720 
   Size:        127     142     451

  Fragments arranged by size: 

                 451     142     127
 
BsaHI Gr'CG_yC

Cuts at:      0     189     720 
   Size:        189     531
 
BsaWI w'CCGG_w

Cuts at:      0      83     720 
   Size:         83     637
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     188     218     415     445     720 
   Size:        188      30     197      30     275

  Fragments arranged by size: 

                 275     197     188      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19     538     720 
   Size:         19     519     182

  Fragments arranged by size: 

                 519     182      19
 
BseYI C'CCAG_C

Cuts at:      0      69     279     351     444     465     631     720 
   Size:         69     210      72      93      21     166      89

  Fragments arranged by size: 

                 210     166      93      89      72      69      21
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     117     166     460     720 
   Size:        117      49     294     260

  Fragments arranged by size: 

                 294     260     117      49
 
BsiWI C'GTAC_G

Cuts at:      0      15     720 
   Size:         15     705
 
Bsp1286I G_dGCh'C

Cuts at:      0     396     720 
   Size:        396     324
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     234     720 
   Size:        234     486
 
BsrFI r'CCGG_y

Cuts at:      0     316     720 
   Size:        316     404
 
BssSI C'ACGA_G

Cuts at:      0      63     268     720 
   Size:         63     205     452

  Fragments arranged by size: 

                 452     205      63
 
BstEII G'GTnAC_C

Cuts at:      0     204     402     720 
   Size:        204     198     318

  Fragments arranged by size: 

                 318     204     198
 
Bsu36I CC'TnA_GG

Cuts at:      0     367     720 
   Size:        367     353
 
BtgI C'CryG_G

Cuts at:      0     489     720 
   Size:        489     231
 
BtsI GCAGTG_nn'

Cuts at:      0     105     720 
   Size:        105     615
 
EaeI y'GGCC_r

Cuts at:      0     152     473     720 
   Size:        152     321     247

  Fragments arranged by size: 

                 321     247     152
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     291     550     646     720 
   Size:        291     259      96      74

  Fragments arranged by size: 

                 291     259      96      74
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     366     720 
   Size:        366     354
 
EcoO109I rG'GnC_Cy

Cuts at:      0     372     392     393     720 
   Size:        372      20       1     327

  Fragments arranged by size: 

                 372     327      20       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     188     220     247     279     720 
   Size:        188      32      27      32     441

  Fragments arranged by size: 

                 441     188      32      32      27
 
HincII GTy'rAC

Cuts at:      0     276     720 
   Size:        276     444
 
KpnI G_GTAC'C

Cuts at:      0     692     720 
   Size:        692      28
 
MscI TGG'CCA

Cuts at:      0     475     720 
   Size:        475     245
 
MspA1I CmG'CkG

Cuts at:      0     180     246     323     333     351     444     465     720 
   Size:        180      66      77      10      18      93      21     255

  Fragments arranged by size: 

                 255     180      93      77      66      21      18      10
            
 
PspOMI G'GGCC_C

Cuts at:      0     392     720 
   Size:        392     328
 
PstI C_TGCA'G

Cuts at:      0     179     231     720 
   Size:        179      52     489

  Fragments arranged by size: 

                 489     179      52
 
PvuII CAG'CTG

Cuts at:      0     180     246     351     444     720 
   Size:        180      66     105      93     276

  Fragments arranged by size: 

                 276     180     105      93      66
 
SbfI CC_TGCA'GG

Cuts at:      0     231     720 
   Size:        231     489
 
SfcI C'TryA_G

Cuts at:      0     175     227     720 
   Size:        175      52     493

  Fragments arranged by size: 

                 493     175      52
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     151     720 
   Size:        151     569
 
SmaI CCC'GGG

Cuts at:      0     109     583     720 
   Size:        109     474     137

  Fragments arranged by size: 

                 474     137     109
 
StyI C'CwwG_G

Cuts at:      0     159     434     720 
   Size:        159     275     286

  Fragments arranged by size: 

                 286     275     159
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      64     502     598     720 
   Size:         64     438      96     122

  Fragments arranged by size: 

                 438     122      96      64
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     441     720 
   Size:        441     279
 
XmaI C'CCGG_G

Cuts at:      0     107     581     720 
   Size:        107     474     139

  Fragments arranged by size: 

                 474     139     107
 

 Enzymes that do cut:

   Acc65I     ApaI     AvaI     BanI    BanII    BbvCI     BglI Bme1580I
     BpmI   Bpu10I     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiWI Bsp1286I    BspMI    BsrFI    BssSI   BstEII   Bsu36I
     BtgI     BtsI     EaeI  Eco57MI    EcoNI EcoO109I    Hin4I   HincII
     KpnI     MscI   MspA1I   PspOMI     PstI    PvuII     SbfI     SfcI
     SfiI     SmaI     StyI    TaqII     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsiEI  BsiHKAI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII   BstAPI
    BstBI    BstXI    BstYI  BstZ17I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     MfeI     MluI     MmeI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     ScaI    SexAI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com